LOCUS       pEF1-AVPS-hOMI-S306A        7087 bp    DNA     circular     11-JAN-2008
DEFINITION  LMBP 4583.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4583
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-JAN-2008
            
COMMENT     VNTNAME|pEF1-AVPS-hOMI-S306A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1-AVPS-hOMI-S306A" 0 7087 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "EcoRI" "HindII" "KpnI" "NaeI" "PstI" "SspI" "XbaI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "2mu ori" 
COMMENT             "AGAAAGTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGTATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACTCTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAAATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCACATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCGCTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGACACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACTGCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAGCGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAATAGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGTAAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGTT" 
COMMENT             "Fragment of pAS3; Length: 981" 0 (CStringList) 0) 
COMMENT         #1=(COligo "5' UTR of AOX1" 
COMMENT             "ACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAA" 
COMMENT             "Fragment of pPP1MFmds1; Length: 165" 0 (CStringList) 0) 
COMMENT         #2=(COligo "ADH1 promoter" 
COMMENT             "TGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAGTTTGCCGCTTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAG" 
COMMENT             "Fragment of pAS3; Length: 404" 0 (CStringList) 0) 
COMMENT         #3=(COligo "ADH1 terminator" 
COMMENT             "GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTC" 
COMMENT             "Fragment of pRH007; Length: 102" 0 (CStringList) 0) 
COMMENT         #4=(COligo "AMP promoter onvolledig" 
COMMENT             "CGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCT" 
COMMENT             "Fragment of pDEST-ECFP; Length: 99 onvolledig" 0 (CStringList) 0) 
COMMENT         #5=(COligo "AMP RBS" "GATAAATGCTTCAATAATATTGAAAAAGGAAGAGT" 
COMMENT             "Fragment of pdECFP; Length: 35" 0 (CStringList) 0) 
COMMENT         #6=(COligo "ampicillin" 
COMMENT             "ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA" 
COMMENT             "Fragment of pEF6-E-mTRAF2; Length: 861" 0 (CStringList) 0) 
COMMENT         #7=(COligo "AOX1 promoter" 
COMMENT             "AACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGCCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTG" 
COMMENT             "Fragment of pPP1MFmds1; Length: 766" 0 (CStringList) 0) 
COMMENT         #8=(COligo "AOX1 terminator" 
COMMENT             "TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGAAGTTCGTTTGTGC" 
COMMENT             "Fragment of pPP1MFmds1; Length: 260" 0 (CStringList) 0) 
COMMENT         #9=(COligo "attR1" 
COMMENT             "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTTTTGTACAAACTTGT" 
COMMENT             "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #10=(COligo "attR2" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #11=(COligo "BK ori" "GAGGCCGAGGCCGCCTCTGCCTC" 
COMMENT              "Fragment of pBK-1; Length: 23 " 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #12=(COligo "blasticidin" 
COMMENT              "ATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 399" 0 (CStringList) 0) 
COMMENT         #13=(COligo "bovine growth hormone polyA" 
COMMENT              "CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG" 
COMMENT              "Fragment of pEF1hCASP-9S-His; Length: 225" 0 (CStringList) 0) 
COMMENT         #14=(COligo "CaGAL1 promoter (1)" 
COMMENT              "AGATCTGATATTGACGAAGAGGAACCAATGTAACGTTACACTGAAGAAAACACACAATAAACGGGAAGAAACGGTGTAAAAGTGTGAAAATAATTTTTGAATATCATTTCCCTTGGTTTAATTCCAAACGAAACGTGTTTTTTTTAGAGAATGGGAATTCTTATTGGATGTCTAGATTGTTTGTTTACTCCAGACTGTGCACAAAAACGTTTGGATGGATGATCAGAAGATATTTTTAGGCTTAGCTCTAAATATAAGAAATGATGCTTGAAAAACCAGACAGAAATTGAGTTTCAAAAATTGGTAATGTGAGGTATTAGTCAACTAACCAAATAACAATGCAAACCGGTTGATACATTTCATTTTGAAAATAATGAAACTGGAATTGGATGACCAGCACACAAACACATAAAGTAATTATGGGAATTAGAAGCGAACATAGAGGAGTACTTGGCCACGAACAGAATACAAGTGGGAACACTATTTTCTCCATTGTTTTAGTTCTGTTTTTTTGTCAGCCTAGTTTTGTGCTATGTGTAAAAAATATTGCCAAGAAAAAAAGCTTGTTTTGTGGCCAGTGTCCGAAAAAAATTTTGGGGAATCTTCGGATTAATTTATGTTTTCATTCCATCGGGGAAAGTGGGGGGGAAAAAATTTTAAGCAGTTCACAAAACCTTCCAAAAAATATATGGACAAAGATGATTGTATTTTCCCGACACCAAAATCATAATTAATTATGAGAAAGTTAAATGTAACGTTACAATTTATGTTTATTTGAAGGTGAAAAGCGATTTATGATTTTTCCGAAATGAAAATTTTTTTTAGGTTTATTTTTTTTGTCGGGCAAAGAAAAACTGAACAAGGATTATTAAAATTTTTGGTGTTTGTTTGTGTCTGGAGAATTCATTCCTCTCTCATCTTCACACAATGTTTAGACATCTGACACGATTCATGATAGTTCGGTTTCCGGGGTTGGTGTTTAGTTTTCGTTTTTCTTTTT" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 1000" 0 (CStringList) 0) 
COMMENT         #15=(COligo "CaGAL1 promoter (2)" 
COMMENT              "TTTTGGAAAGAATGTTTTAGCTCATTGGTTTTCTTTCTTCATTCAATAGTTTTGAAAGAATTTGCCCACTTGTTATTACAATCATATAAAATTAAACTTTGATATAAAATAGAGTTTGAAAGTTTCCCAGATCCTTTTTGATTTCTTTGTAAATTTTTTTTTCTCCCACATATACACACATACAAACCGATTTTTATAAGAAAGAGTTATACC" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 213" 0 (CStringList) 0) 
COMMENT         #16=(COligo "CaMV promoter" 
COMMENT              "GATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCT" 
COMMENT              "Fragment of pEX1SOD; Length: 947" 0 (CStringList) 0) 
COMMENT         #17=(COligo "CaURA3" 
COMMENT              "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #18=(COligo "ccdB" 
COMMENT              "ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA" 
COMMENT              "Fragment of pRH001; Length: 306" 0 (CStringList) 0) 
COMMENT         #19=(COligo "CEN6/ARSH4 ori" 
COMMENT              "CTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 525" 0 (CStringList) 0) 
COMMENT         #20=(COligo "chloramphenicol" 
COMMENT              "ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA" 
COMMENT              "Fragment of pRH001; Length: 660" 0 (CStringList) 0) 
COMMENT         #21=(COligo "CYC1 terminator" 
COMMENT              "ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAG" 
COMMENT              "Fragment of pAS2; Length: 276  (laatste N: G (276) ipv A (275) -> midden HindIII)" 
COMMENT              0 (CStringList) 0) 
COMMENT         #22=(COligo "CYH2" 
COMMENT              "ATGCCTTCCAGATTCACTAAGACTAGAAAGCACAGAGGTCACGTCTCAGCCGGTAAAGGTCGTATCGGTAAGCACAGAAAGCACCCCGGTGGTAGAGGTATGGCCGGTGGTCAACATCACCACAGAATTAACATGGATAAATACCATCCAGGTTATTTCGGTAAGGTTGGTATGAGATACTTCCACAAGCAACAAGCTCATTTCTGGAAGCCAGTCTTGAACTTGGACAAATTGTGGACATTGATCCCAGAAGACAAGAGAGACCAATACTTGAAATCTGCTTCTAAGGAAACTGCTCCAGTTATTGACACTTTGGCAGCCGGTTACGGTAAGATCTTGGGTAAGGGTAGAATTCCAAATGTTCCAGTTATCGTCAAAGCTAGATTCGTCTCCAAGTTGGCTGAAGAAAAAATCAGAGCTGCTGGTGGTGTTGTTGAATTGATCGCTTAA" 
COMMENT              "Fragment of pAS2; Length: 450" 0 (CStringList) 0) 
COMMENT         #23=(COligo "E-tag" "GGNGCNCCNGTNCCNTAYCCNGAYCCNYTNGARCCNCGN" 
COMMENT              " Fragment of pYD1TSE ; Length: 39" 0 (CStringList) 0) 
COMMENT         #24=(COligo "ECFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pECFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #25=(COligo "EGFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEGFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #26=(COligo "EK recognition site" "GAYGAYGAYGAYAAR" "Length: 15 bp" 0 
COMMENT              (CStringList) 0) 
COMMENT         #27=(COligo "EM7 promoter" 
COMMENT              "GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACC" 
COMMENT              "Fragment of pPICZTS; Length: 66, as in the pEF6 plasmids" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #28=(COligo "EYFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEYFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #29=(COligo "f1 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pAS2; Length: 456" 0 (CStringList) 0) 
COMMENT         #30=(COligo "fd terminator" 
COMMENT              "TAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCC" 
COMMENT              "Fragment of bacteriophage_fd; Length: 82, zoals in pMa58" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #31=(COligo "FLAG-tag" "GAYTAYAARGAYGAYGAYGAYAAR" 
COMMENT              " Fragment of pCAGGS-FLAGmA20; Length: 24" 0 (CStringList) 0) 
COMMENT         #32=(COligo "FLD1 promoter start" 
COMMENT              "GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGGATATGCCGCCTATAGGATAAAAACAGGAGA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #33=(COligo "FLD1 promoter stop" 
COMMENT              "TTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCTCCAACTTTGCTTGTTCATACAATTCTTGATATTCACA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #34=(COligo "FLP terminator" 
COMMENT              "GTACGCATTTAAGCATAAACACGCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTGTATGTGC" 
COMMENT              "Fragment of pSCGALyEGFP1 compl; Length: 105" 0 (CStringList) 0) 
COMMENT         #35=(COligo "GAL1 promoter" 
COMMENT              "AAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 384" 0 (CStringList) 0) 
COMMENT         #36=(COligo "GAL10/CYC1 promoter" 
COMMENT              "ATAACCATAAAAGCTAGTATTGTAGAATCTTTATTGTTCGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGGAACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGGCTTCTAATCCGTACTTCAATATAGCAATGAGCAGTTAAGCGTATTACTGAAAGTTCCAAAGAGAAGGTTTTTTTAGGCTAATCGACCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACAT" 
COMMENT              "Fragment of pEMBLyex4; Length: 408" 0 (CStringList) 0) 
COMMENT         #37=(COligo "GALad" 
COMMENT              "ATGGATAAAGCGGAATTAATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTACCGCCGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAG" 
COMMENT              "Fragment of pGAD-mCASP-11; Length: 408" 0 (CStringList) 0) 
COMMENT         #38=(COligo "GALbd" 
COMMENT              "ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCG" 
COMMENT              "Fragment of pAS3; Length: 441" 0 (CStringList) 0) 
COMMENT         #39=(COligo "GALL promoter" 
COMMENT              "TGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of p415GALLmBAX; Length: 369" 0 (CStringList) 0) 
COMMENT         #40=(COligo "GAP promoter" 
COMMENT              "AGATCTTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTAT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 483" 0 (CStringList) 0) 
COMMENT         #41=(COligo "GST" 
COMMENT              "ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA" 
COMMENT              "Fragment of pGEXGSTp65(12-317); Length: 654" 0 (CStringList) 0) 
COMMENT         #42=(COligo "GUS start" 
COMMENT              "ATGGTCCGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTT" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #43=(COligo "GUS stop" 
COMMENT              "CACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATAA" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #44=(COligo "HA epitope" "TAYCCNTAYGAYGTNCCNGAYTAYGCN" 
COMMENT              "Fragment of pAS2; Length: 27" 0 (CStringList) 0) 
COMMENT         #45=(COligo "hCMV-IE promoter and enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT              "Fragment of pCDM8; Length: 584" 0 (CStringList) 0) 
COMMENT         #46=(COligo "hEF1 promoter (1)" 
COMMENT              "GCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACT" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #47=(COligo "hEF1 promoter (2)" 
COMMENT              "GAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 144" 0 (CStringList) 0) 
COMMENT         #48=(COligo "His-tag" "CAYCAYCAYCAYCAYCAY" "" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #49=(COligo "HIS3 start" 
COMMENT              "ATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGA" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #50=(COligo "HIS3 stop" 
COMMENT              "TAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAG" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #51=(COligo "hOMI (1)" 
COMMENT              "ATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGACCCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTCACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACTCTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCTCGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCC" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #52=(COligo "hOMI (2)" 
COMMENT              "CTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 377" 0 (CStringList) 0) 
COMMENT         #53=(COligo "HSV-TK polyA" 
COMMENT              "AACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCAC" 
COMMENT              "Fragment of pEYFP-N1; Length: 70" 0 (CStringList) 0) 
COMMENT         #54=(COligo "HSV-TK promoter" 
COMMENT              "CCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGTGTGGCCTCGA" 
COMMENT              "Fragment of p220.2; Length: 105" 0 (CStringList) 0) 
COMMENT         #55=(COligo "intron AG promoter start" 
COMMENT              "GTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGC" 
COMMENT              "Fragment of pCAGGS; Length: 100" 0 (CStringList) 0) 
COMMENT         #56=(COligo "intron AG promoter stop" 
COMMENT              "TCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG" 
COMMENT              "Fragment of pCAGGS; Length: 100" 0 (CStringList) 0) 
COMMENT         #57=(COligo "intron hEF1 promoter" 
COMMENT              "GTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAG" 
COMMENT              "Fragment of pEF1-hOMI; Length: 939" 0 (CStringList) 0) 
COMMENT         #58=(COligo "IRES start" 
COMMENT              "CCCCCCCCCCCCCCCCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCC" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #59=(COligo "IRES stop" 
COMMENT              "CTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAA" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #60=(COligo "kanamycin" 
COMMENT              "ATGAGCCATATTCAACGGGAAACGTCTTGCTCAAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" 
COMMENT              "Fragment of pRH001; Length: 816" 0 (CStringList) 0) 
COMMENT         #61=(COligo "lac promoter" 
COMMENT              "GCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG" 
COMMENT              "from pUC18; 84 bp." 0 (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #62=(COligo "lacA" 
COMMENT              "TTGAACATGCCAATGACCGAAAGAATAAGAGCAGGCAAGCTATTTACCGATATGTGCGAAGGCTTACCGGAAAAAAGACTTCGTGGGAAAACGTTAATGTATGAGTTTAATCACTCGCATCCATCAGAAGTTGAAAAAAGAGAAAGCCTGATTAAAGAAATGTTTGCCACGGTAGGGGAAAACGCCTGGGTAGAACCGCCTGTCTATTTCTCTTACGGTTCCAACATCCATATAGGCCGCAATTTTTATGCAAATTTCAATTTAACCATTGTCGATGACTACACGGTAACAATCGGTGATAACGTACTGATTGCACCCAACGTTACTCTTTCCGTTACGGGACACCCTGTACACCATGAATTGAGAAAAAACGGCGAGATGTACTCTTTTCCGATAACGATTGGCAATAACGTCTGGATCGGAAGTCATGTGGTTATTAATCCAGGCGTCACCATCGGGGATAATTCTGTTATTGGCGCGGGTAGTATCGTCACAAAAGACATTCCACCAAACGTCGTGGCGGCTGGCGTTCCTTGTCGGGTTATTCGCGAAATAAACGACCGGGATAAGCACTATTATTTCAAAGATTATAAAGTTGAATCGTCAGTTTAA" 
COMMENT              "Fragment of pLacDN10HIFNG; Length: 612" 0 (CStringList) 0) 
COMMENT         #63=(COligo "lacI promoter" 
COMMENT              "GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of pREP4; Length: 78" 0 (CStringList) 0) 
COMMENT         #64=(COligo "lacI start" 
COMMENT              "GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #65=(COligo "lacI stop" 
COMMENT              "CACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #66=(COligo "lacIq promoter" 
COMMENT              "GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of p714; Length: 78" 0 (CStringList) 0) 
COMMENT         #67=(COligo "lacUV5 promoter" 
COMMENT              "CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGG" 
COMMENT              "Fragment of p714; Length: 57" 0 (CStringList) 0) 
COMMENT         #68=(COligo "lacZ RBS" "AATTGTGAGCGGATAACAATTTCACACAGGAAACAGCT" 
COMMENT              "Fragment of pGBF-NLScre; Length: 38" 0 (CStringList) 0) 
COMMENT         #69=(COligo "lacZ start" 
COMMENT              "ATGGGGCCCAAGAAGAAACGCAAAGTGGGGAGCATGGGGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #70=(COligo "lacZ stop" 
COMMENT              "GGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTACAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #71=(COligo "LEU2 start" 
COMMENT              "ATGTTTGTTCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTC" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #72=(COligo "LEU2 stop" 
COMMENT              "TTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAA" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #73=(COligo "loxP" "ATAACTTCGTATAGCATACATTATACGAAGTT" 
COMMENT              "Fragment of pCALNL5; Length: 32" 0 (CStringList) 0) 
COMMENT         #74=(COligo "LUC start" 
COMMENT              "ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACG" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #75=(COligo "LUC stop" 
COMMENT              "TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #76=(COligo "M13 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pCS4; Length: 456" 0 (CStringList) 0) 
COMMENT         #77=(COligo "mBAX" 
COMMENT              "ATGGACGGGTCCGGGGAGCAGCTTGGGAGCGGCGGGCCCACCAGCTCTGAACAGATCATGAAGACAGGGGCCTTTTTGCTACAGGGTTTCATCCAGGATCGAGCAGGGAGGATGGCTGGGGAGACACCTGAGCTGACCTTGGAGCAGCCGCCCCAGGATGCGTCCACCAAGAAGCTGAGCGAGTGTCTCCGGCGAATTGGAGATGAACTGGATAGCAATATGGAGCTGCAGAGGATGATTGCTGACGTGGACACGGACTCCCCCCGAGAGGTCTTCTTCCGGGTGGCAGCTGACATGTTTGCTGATGGCAACTTCAACTGGGGCCGCGTGGTTGCCCTCTTCTACTTTGCTAGCAAACTGGTGCTCAAGGCCCTGTGCACTAAAGTGCCCGAGCTGATCAGAACCATCATGGGCTGGACACTGGACTTCCTCCGTGAGCGGCTGCTTGTCTGGATCCAAGACCAGGGTGGCTGGGAAGGCCTCCTCTCCTACTTCGGGACCCCCACATGGCAGACAGTGACCATCTTTGTGGCTGGAGTCCTCACCGCCTCGCTCACCATCTGGAAGAAGATGGGCTGA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 579" 0 (CStringList) 0) 
COMMENT         #78=(COligo "mob'" 
COMMENT              "ATGGCGGCATACGCGATCATGCGATGCAAGAAGCTGGCGAAAATGGGCAACGTGGCGGCCAGTCTCAAGCACGCCTACCGCGAGCGCGAGACGCCCAACGCTGACGCCAGCAGGACGCCAGAGAACGAGCACTGGGCGGCCAGCAGCACCGATGAAGCGATGGGCCGACTGCGCGAGTTGCTGCCAGAGAAGCGGCGCAAGGACGCTGTGTTGGCGGTCGAGTACGTCATGACGGCCAGCCCGGAATGGTGGAAGTCGGCCAGCCAAGAACAGCAGGCGGCGTTCTTCGAGAAGGCGCACAAGTGGCTGGCGGACAAGTACGGGGCGGATCGCATCGTGACGGCCAGCATCCACCGTGACGAAACCAGCCCGCACATGACCGCGTTCGTGGTGCCGCTGACGCAGGACGGCAGGCTGTCGGCCAAGGAGTTCATCGGCAACAAAGCGCAGATGACCCGCGACCAGACCACGTTTGCGGCCGCTGTGGCCGATCTAGGGCTGCAACGGGGCATCGAGGGCAGCAAGGCACGTCACACGCGCATTCAGGCGTTCTACGAGGCCCTGGAGCGGCCACCAGTGGGCCACGTCACCATCAGCCCGCAAGCGGTCGAGCCACGCGCCTATGCACCGCAGGGATTGGCCGAAAAGCTGGGAATCTCAAAGCGCGTTGAGACGCCGGAAGCCGTGGCCGACCGGCTGACAAAAGCGGTTCGGCAGGGGTATGAGCCTGCCCTACAGGCCGCCGCAGGAGCGCGTGAGATGCGCAAGAAGGCCGATCAAGCCCAAGAGACGGCCCGAGACCTTCGGGAGCGCCTGAAGCCCGTTCTGGACGCCCTGGGGCCGTTGAATCGGGATATGCAGGCCAAGGCCGCCGCGATCATCAAGGCCGTGGGCGAAAAGCTGCTGACGGAACAGCGGGAAGTCCAGCGCCAGAAACAGGCCCAGCGCCAGCAGGAACGCGGGCGCG" 
COMMENT              "incomplete but functional mobilization gene; 6 C-terminal codons missing" 
COMMENT              0 (CStringList) 0) 
COMMENT         #79=(COligo "Myc epitope" "GARCARAARYTNATHWSNGARGARGAYYTN" 
COMMENT              "Fragment of MYC-TAG; Length: 30" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #80=(COligo "neomycin" 
COMMENT              "ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA" 
COMMENT              "Fragment of pCDNA-mCASP-8; Length: 795" 0 (CStringList) 0) 
COMMENT         #81=(COligo "nos promoter start" 
COMMENT              "GATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGATTGAA" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #82=(COligo "nos promoter stop" 
COMMENT              "AGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAA" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #83=(COligo "ocs terminator start" 
COMMENT              "CTGCTTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATC" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #84=(COligo "ocs terminator stop" 
COMMENT              "GTGGGCCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGACAAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAG" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #85=(COligo "p15A ori start" 
COMMENT              "GGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGT" 
COMMENT              "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #86=(COligo "p15A ori stop" 
COMMENT              "GTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTT" 
COMMENT              "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #87=(COligo "parA" 
COMMENT              "TTGAAGGTCGCCCGCATCTACCTGCGCGCCAGTACGGACGAGCAGAATCTTGAACGCCAGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTTGCGGAGAAGATCGACCGCATCAGCCGCTTGCCGTTGGCCGAGGCCGAGCGCCTGGTTGCGTCGATCCGGGCCAAAGGGGCCAAGCTGGCCGTGCCTGGCGTGGTGGACCTGTCGGAGCTGGCCGCCGAGGCGAACGGAGTGGCGAAAATCGTTCTGGAATCCGTCCAGGACATGCTTTTGAAGCTCGCCTTGCAGATGGCCCGCGACGACTACGAGGATCGGCGCGAGCGTCAACGTCAGGGTGTCCAGTTGGCGAAGGCCGCCGGCCGCTACACCGGCCGCAAACGTGACGCCGGCATGCACGACCGCATCATCACGCTTCGCTCCGGCGGATCGAGCATTGCCAAGACGGCCAAGCTGGTCGGATGCAGCCCGAGCCAGGTCAAACGAGTGTGGGCGGCCTGGAACGCGCAGCAGCAAAAATAA" 
COMMENT              "Fragment of pRH001; Length: 762" 0 (CStringList) 0) 
COMMENT         #88=(COligo "parB" 
COMMENT              "ATGAAGCGCCGAAGCTACGCCATGCTGCGCGCCGCTGCCGCGCTGGCCGTCCTGGTCGTTGCCTCGCCGGCATGGGCCGAGCTGCGCGGCGAGGTCGTGCGCATCATCGACGGCGACACCATCGACGTGCTGGTAGACAAGCAGCCGGTGCGCGTGCGCCTGGTGGACATTGACGCGCCGGAAAAGCGGCAAGCCTTCGGCGAACGTGCGCGCCAGGCGCTGGCCGGCATGGTGTTCCGCCGGCACGTCCTGGTCGACGAGAAGGACACCGACCGTTACGGCCGCACGCTGGGCACCGTGTGGGTCAACATGGAGCTGGCCAGCCGGCCGCCGCAGCCGCGCAACGTCAACGCCGCGATGGTTCACCAGGGCATGGCGTGGGCCTATCGCTTCCACGGCCGCGCGGCCGACCCTGAAATGCTGCGGCTCGAACAGGAGGCGCGAGGCAAGCGCGTCGGCCTCTGGTCCGATCCGCACGCCGTCGAGCCGTGGAAATGGCGACGCGAGAGCAACAACCGGAGGGACGAAGGTTGA" 
COMMENT              "Fragment of pRH001; Length: 534" 0 (CStringList) 0) 
COMMENT         #89=(COligo "parC" 
COMMENT              "ATGGGTATACCCAATTTGACCAAGGGGGACGCGATGAGGGCGGCCAAGCACTACCGACAACTTCTATCCATCGACTTCAACATCGAGGCGCTGGCCTTCGTGCCTGGACCCGACGGCACACGCGGCCGGCGCATCCACGTCCTGGGGCGCGAGGTCCGCGACCGGCCCGGCCTGGTCGAGTACCTTTCGCCGGCGTTCGGCTCGCGGGTGGCGCTGGACGGCTACTGCAAGGCCAATTTCGATGCAGTGCTGCACCTGGCGTACCCCGATCATCAGCAATGGGGCCACGCATGA" 
COMMENT              "Fragment of pRH001; Length: 294" 0 (CStringList) 0) 
COMMENT         #90=(COligo "parD" 
COMMENT              "ATGAGCCGCCTGACAATCGACATGACGGACCAGCAGCACCAGAGCCTGAAAGCCCTGGCCGCCTTGCAGGGCAAGACCATTAAGCAATACGCCCTCGAACGTCTGTTCCCCGGTGACGCTGATGCCGATCAGGCATGGCAGGAACTGAAAACCATGCTGGGGAACCGCATCAACGATGGGCTTGCCGGCAAGGTGTCCACCAAGAGCGTCGGCGAAATTCTTGATGAAGAACTCAGCGGGGATCGCGCTTGA" 
COMMENT              "Fragment of pMR10cat; Length: 252" 0 (CStringList) 0) 
COMMENT         #91=(COligo "parE" 
COMMENT              "TTGACGGCCTACATCCTCACGGCTGAGGCCGAAGCCGATCTACGCGGCATCATCCGCTACACGCGCCGGGAGTGGGGCGCGGCGCAGGTGCGCCGCTATATCGCTAAGCTGGAACAGGGCATAGCCAGGCTTGCCGCCGGCGAAGGCCCGTTTAAGGACATGAGCGAACTCTTTCCCGCGCTGCGGATGGCCCGCTGCGAACACCACTACGTTTTTTGCCTGCCGCGTGCGGGCGAACCCGCGTTGGTCGTGGCGATCCTGCATGAGCGCATGGACCTCATGACGCGACTTGCCGACAGGCTCAAGGGCTGA" 
COMMENT              "Fragment of pMR10cat; Length: 312" 0 (CStringList) 0) 
COMMENT         #92=(COligo "pBBR1 ori" 
COMMENT              "ATGGCCACGCAGTCCAGAGAAATCGGCATTCAAGCCAAGAACAAGCCCGGTCACTGGGTGCAAACGGAACGCAAAGCGCATGAGGCGTGGGCCGGGCTTATTGCGAGGAAACCCACGGCGGCAATGCTGCTGCATCACCTCGTGGCGCAGATGGGCCACCAGAACGCCGTGGTGGTCAGCCAGAAGACACTTTCCAAGCTCATCGGACGTTCTTTGCGGACGGTCCAATACGCAGTCAAGGACTTGGTGGCCGAGCGCTGGATCTCCGTCGTGAAGCTCAACGGCCCCGGCACCGTGTCGGCCTACGTGGTCAATGACCGCGTGGCGTGGGGCCAGCCCCGCGACCAGTTGCGCCTGTCGGTGTTCAGTGCCGCCGTGGTGGTTGATCACGACGACCAGGACGAATCGCTGTTGGGGCATGGCGACCTGCGCCGCATCCCGACCCTGTATCCGGGCGAGCAGCAACTACCGACCGGCCCCGGCGAGGAGCCGCCCAGCCAGCCCGGCATTCCGGGCATGGAACCAGACCTGCCAGCCTTGACCGAAACGGAGGAATGGGAACGGCGCGGGCAGCAGCGCCTGCCGATGCCCGATGAGCCGTGTTTTCTGGACGATGGCGAGCCGTTGGAGCCGCCGACACGGGTCACGCTGCCGCGCCGGTAG" 
COMMENT              "" 0 (CStringList) 0) 
COMMENT         #93=(COligo "pColE1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCTGCTACGTGGCCTTCTTCCTGTTCCTGGTCTTTTGCTCACATGTTCTTTCCGGCCTTATCCCCTGATTCTGTGGATAACTGTGTTACCGTTTTTGTGTGAGTCAGTACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAAAGC" 
COMMENT              "Fragment of pPLa2311trpA1; Length: 392" 0 (CStringList) 0) 
COMMENT         #94=(COligo "PL promoter" 
COMMENT              "TCTCTCACCTACCAAACAATGCCCCCCTGCAAAAAATAAATTCATATAAAAAACATACAGATAACCATCTGCGGTGATAAATTATCTCTGGCGGTGTTGACATAAATACCACTGGCGGTGATACTGAGCAC" 
COMMENT              "Fragment of pLT-mCASP-1p30-1; Length: 131" 0 (CStringList) 0) 
COMMENT         #95=(COligo "pMB1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" 
COMMENT              "Fragment of pRH007; Length: 403" 0 (CStringList) 0) 
COMMENT         #96=(COligo "polyoma ori" 
COMMENT              "CTGTTTTTTTTAGTATTAAGCAGAGGCCGGGGACCCCTGGGCCCGCTTACTCTGGAGAAAAAGAAGAGAGGCATTGTAGAGGCTTCCAGAGGCAACTTGTCAAAACAGGACTG" 
COMMENT              "Fragment of pCDM92; Length: 113" 0 (CStringList) 0) 
COMMENT         #97=(COligo "ppMF" 
COMMENT              "ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 243" 0 (CStringList) 0) 
COMMENT         #98=(COligo "R6K ori" 
COMMENT              "TCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTCAGTGACACAGGAACACTTAACGGCTGACAT" 
COMMENT              "Fragment of pKNG101; Length: 388" 0 (CStringList) 0) 
COMMENT         #99=(COligo "RK2 ori" 
COMMENT              "GGCCGCCGGCGTTGTGGATACCTCGCGGAAAACTTGGCCCTCACTGACAGATGAGGGGCGGACGTTGACACTTGAGGGGCCGACTCACCCGGCGCGGCGTTGACAGATGAGGGGCAGGCTCGATTTCGGCCGGCGACGTGGAGCTGGCCAGCCTCGCAAATCGGCGAAAACGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGACAAGCCTGGGGATAAGTGCCCTGCGGTATTGACACTTGAGGGGCGCGACTACTGACAGATGAGGGGCGCGATCCTTGACACTTGAGGGGCAGAGTGCTGACAGATGAGGGGCGCACCTATTGACATTTGAGGGGCTGTCCACAGGCAGAAAATCCAGCATTTGCAAGGGTTTCCGCCCGTTTTTCGGCCACCGCTAACCTGTCTTTTAACCTGCTTTTAAACCAATATTTATAAACCTTGTTTTTAACCAGGGCTGCGCCCTGTGCGCGTGACCGCGCACGCCGAAGGGGGGTGCCCCCCCTTCTCGAACCCTCCCGG" 
COMMENT              "Fragment of pRH001; Length: 526" 0 (CStringList) 0) 
COMMENT         #100=(COligo "RK2/RP4 oriT" 
COMMENT               "CGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG" 
COMMENT               "Fragment of pRH001; Length: 111" 0 (CStringList) 0) 
COMMENT         #101=(COligo "rop" 
COMMENT               "GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA" 
COMMENT               "Fragment of pBR322; Length: 192" 0 (CStringList) 0) 
COMMENT         #102=(COligo "SP6 promoter" "ATTTAGGTGACACTATA" 
COMMENT               "Fragment of pdCS2-MT ; Length: 17" 0 (CStringList) 0) 
COMMENT         #103=(COligo "Strep-tag" "GCTTGGCGTCACCCGCAGTTCGGTGGT" 
COMMENT               "pUChGNTI2: 27 nucleotides" 0 (CStringList) 0) 
COMMENT         #104=(COligo "supF" 
COMMENT               "TCTTTCTCAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATAAGGGAGCAGGCCAGTAAAAGCATTACCCGTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTCTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCGAAAGAAT" 
COMMENT               "Fragment of pCDM92; Length: 203" 0 (CStringList) 0) 
COMMENT         #105=(COligo "SV40 early promoter" 
COMMENT               "CTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCC" 
COMMENT               "Fragment of pEF6/Myc-HisA; Length: 282" 0 (CStringList) 0) 
COMMENT         #106=(COligo "SV40 ori" 
COMMENT               "GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCKGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT" 
COMMENT               "Fragment of SV40; Length: 76" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #107=(COligo "SV40 polyA early" 
COMMENT               "AATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTT" 
COMMENT               "Fragment of pdEYFP; Length: 97" 0 (CStringList) 0) 
COMMENT         #108=(COligo "T1 terminator" 
COMMENT               "ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT" 
COMMENT               "Fragment of pDONR201; Length: 44" 0 (CStringList) 0) 
COMMENT         #109=(COligo "T2 terminator" "AGAAGGCCATCCTGACGGATGGCCTTTT" 
COMMENT               "Fragment of pDONR201; Length: 28" 0 (CStringList) 0) 
COMMENT         #110=(COligo "T3 promoter" "AATTAACCCTCACTAAA" 
COMMENT               "Fragment of pLT-mCASP-14" 0 (CStringList) 0) 
COMMENT         #111=(COligo "T7 promoter" "TAATACGACTCACTATA" 
COMMENT               "Fragment of pGEM-TAX1BP1-S; Length: 17" 0 (CStringList) 0) 
COMMENT         #112=(COligo "T7 RBS" 
COMMENT               "GGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAT" 
COMMENT               "Fragment of pLT10Bgal; Length: 63" 0 (CStringList) 0) 
COMMENT         #113=(COligo "T7 terminator" 
COMMENT               "CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG" 
COMMENT               "Fragment of pGBKT7; Length: 48" 0 (CStringList) 0) 
COMMENT         #114=(COligo "tac promoter" "GTTGACAATTAATCATCGGCTCGTATAATGTGTGG" 
COMMENT               "Fragment of pGEX5X1; Length: 35" 0 (CStringList) 0) 
COMMENT         #115=(COligo "TEF1 E. gossypii" 
COMMENT               "GGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCTCCATGTC" 
COMMENT               "E. gossypii TEF1alpha promoter" 0 (CStringList) 0) 
COMMENT         #116=(COligo "TEF1 terminator start" 
COMMENT               "AGTAACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATT" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #117=(COligo "TEF1 terminator stop" 
COMMENT               "ATATTTTTTTTGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #118=(COligo "TEF1a promoter start" 
COMMENT               "CACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATA" 
COMMENT               "Fragment of pFLD; Length: 100" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #119=(COligo "TEF1a promoter stop" 
COMMENT               "GGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGYTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG" 
COMMENT               "Fragment of pFLD; Length: 100" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB" "nagekeken 06/2005 AO") 0) 
COMMENT         #120=(COligo "TET start" 
COMMENT               "ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGG" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #121=(COligo "TET stop" 
COMMENT               "CACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGA" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #122=(COligo "thrombin recognition site" "CTGGTTCCGCGTGGATCC" 
COMMENT               "Fragment of pGEX-4T2; Length: 18" 0 (CStringList) 0) 
COMMENT         #123=(COligo "TRP1" 
COMMENT               "ATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAG" 
COMMENT               "Fragment of pAS2; Length: 675" 0 (CStringList) 0) 
COMMENT         #124=(COligo "URA3" 
COMMENT               "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT               "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0) 
COMMENT         #125=(COligo "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT               "length: 42 bp" 0 (CStringList) 0) 
COMMENT         #126=(COligo "VSV epitope" "TACACCGACATCGAGATGAACCGGTTGGGCAAG" 
COMMENT               "Fragment of pCR3VSV-CED-4; Length: 33" 0 (CStringList) 0) 
COMMENT         #127=(COligo "XGPRT" 
COMMENT               "ATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAAGTGCTGAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAA" 
COMMENT               "Fragment of pDelta1; Length: 459" 0 (CStringList) 0) 
COMMENT         #128=(COligo "Xpress epitope" "GAYYTNTAYGAYGAYGAYGAYAAR" 
COMMENT               "Fragment of pYD1TSE ; Length: 24" 0 (CStringList) 0) 
COMMENT         #129=(COligo "zeocin" 
COMMENT               "ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 375" 0 (CStringList) 0) 
COMMENT         #130=(COligo "\337-globin polyA" 
COMMENT               "GATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGSTAATAAAGGAAATTTATTTTCATTGC" 
COMMENT               "Fragment of pBI-G; Length: 92" 0 (CStringList) 0)) 1 "{(0,1),2}" 
COMMENT        0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1 7087 1 7087 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #131=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2367 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "HindII" "gtyrac" 4 0 4 123 0 357 0 2256 0 
COMMENT              5033 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1718 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "NaeI" "gccggc" 4 0 5 1176 0 2241 0 3213 0 
COMMENT              4554 0 4839 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 790 0 1295 0 2123 0 
COMMENT              4105 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SspI" "aatatt" 4 0 1 4 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2701 0 0 "") 
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FEATURES             Location/Qualifiers
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     intron          704..1642
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     misc_marker     2718..2759
                     /vntifkey="22"
                     /label=V5\epitope
     misc_marker     2769..2786
                     /vntifkey="22"
                     /label=His-tag
     terminator      2815..3039
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
     rep_origin      3085..3513
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3518..3799
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3756..3831
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      4893..4967
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4893..4967)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(6213..7073)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             3923..4717
                     /vntifkey="4"
                     /label=neomycin
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     rep_origin      complement(5281..5672)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1719..2699
                     /vntifkey="4"
                     /label=mature\hOMI\mutant
     promoter        complement(5106..5189)
                     /vntifkey="30"
                     /label=lac\promoter
     mutation        2238..2243
                     /vntifkey="62"
                     /label=S306A
                     /note="WT: TCT.GGA"
BASE COUNT     1524 a      1860 c      1937 g      1766 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccat ggccgtccct agcccgccgc 
     1741 ccgcttctcc ccggagtcag tacaacttca tcgcagatgt ggtggagaag acagcacctg 
     1801 ccgtggtcta tatcgagatc ctggaccggc accctttctt gggccgcgag gtccctatct 
     1861 cgaacggctc aggattcgtg gtggctgccg atgggctcat tgtcaccaac gcccatgtgg 
     1921 tggctgatcg gcgcagagtc cgtgtgagac tgctaagcgg cgacacgtat gaggccgtgg 
     1981 tcacagctgt ggatcccgtg gcagacatcg caacgctgag gattcagact aaggagcctc 
     2041 tccccacgct gcctctggga cgctcagctg atgtccggca aggggagttt gttgttgcca 
     2101 tgggaagtcc ctttgcactg cagaacacga tcacatccgg cattgttagc tctgctcagc 
     2161 gtccagccag agacctggga ctcccccaaa ccaatgtgga atacattcaa actgatgcag 
     2221 ctattgattt tggaaacgcc ggcggtcccc tggttaacct ggatggggag gtgattggag 
     2281 tgaacaccat gaaggtcaca gctggaatct cctttgccat cccttctgat cgtcttcgag 
     2341 agtttctgca tcgtggggaa aagaagaatt cctcctccgg aatcagtggg tcccagcggc 
     2401 gctacattgg ggtgatgatg ctgaccctga gtcccagcat ccttgctgaa ctacagcttc 
     2461 gagaaccaag ctttcccgat gttcagcatg gtgtactcat ccataaagtc atcctgggct 
     2521 cccctgcaca ccgggctggt ctgcggcctg gtgatgtgat tttggccatt ggggagcaga 
     2581 tggtacaaaa tgctgaagat gtttatgaag ctgttcgaac ccaatcccag ttggcagtgc 
     2641 agatccggcg gggacgagaa acactgacct tatatgtgac ccctgaggtc acagaatgat 
     2701 ctagagggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc 
     2761 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc 
     2821 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
     2881 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
     2941 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
     3001 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
     3061 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     3121 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     3181 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     3241 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     3301 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     3361 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     3421 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     3481 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
     3541 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     3601 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     3661 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     3721 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     3781 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     3841 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     3901 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     3961 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     4021 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     4081 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     4141 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     4201 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     4261 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     4321 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     4381 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     4441 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     4501 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     4561 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     4621 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     4681 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa 
     4741 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 
     4801 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     4861 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 
     4921 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4981 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 
     5041 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
     5101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 
     5161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
     5221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     5281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     5341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     5401 agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     5461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     5521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     5581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     5641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     5701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     5761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     5821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     5881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     5941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     6001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     6061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     6121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     6181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     6241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     6301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     6361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     6421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     6481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     6541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     6601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     6661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     6721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     6781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     6841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     6901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     6961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     7021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     7081 ctttttc 
//
