LOCUS       pUHD-2FKBP-link-hFADD 5193 bp dsDNACircular
DEFINITION  LMBP 4598.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        4..369
                     /label=hCMVT\promoter
     misc_feature    1163..1180
                     /label=GSGGGS\linker
     CDS             1187..1810
                     /label=hFADD
     misc_difference 1817..1843
                     /label=HA\epitope
     misc_difference 461..502
                     /label=myristoylation-targeting\sequence
     CDS             509..829
                     /label=hFKBP12
     CDS             836..1156
                     /label=hFKBP12
     terminator      2590..2686
                     /label=SV40\polyA\late
     terminator      complement(2590..2686)
                     /label=SV40\polyA\early
     rep_origin      complement(3190..3592)
                     /label=pMB1\ori
     CDS             complement(4133..4993)
                     /label=AMP
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc 
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa 
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc 
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag 
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag 
      301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt 
      361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca 
      421 ccgggaccga tccagcctcc gcggccccga attcgcgcgt atggggagta gcaagagcaa 
      481 gcctaaggac cccagccagc gctctagagg agtgcaggtg gaaaccatct ccccgggaga 
      541 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga 
      601 agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg 
      661 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag 
      721 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat 
      781 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaag tcgagggcgt 
      841 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg 
      901 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag 
      961 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg 
     1021 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta 
     1081 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga 
     1141 gcttctaaaa ctggaaacta gagggtctgg aggcggatcc tctagtatgg acccgttcct 
     1201 ggtgctgctg cactcggtgt cgtccagcct gtcgagcagc gagctgaccg agctcaagtt 
     1261 cctatgcctc gggcgcgtgg gcaagcgcaa gctggagcgc gtgcagagcg gcctagacct 
     1321 cttctccatg ctgctggagc agaacgacct ggagcccggg cacaccgagc tcctgcgcga 
     1381 gctgctcgcc tccctgcggc gccacgacct gctgcggcgc gtcgacgact tcgaggcggg 
     1441 ggcggcggcc ggggccgcgc ctggggaaga agacctgtgt gcagcattta acgtcatatg 
     1501 tgataatgtg gggaaagatt ggagaaggct ggctcgtcag ctcaaagtct cagacaccaa 
     1561 gatcgacagc atcgaggaca gatacccccg caacctgaca gagcgtgtgc gggagtcact 
     1621 gagaatctgg aagaacacag agaaggagaa cgcaacagtg gcccacctgg tgggggctct 
     1681 caggtcctgc cagatgaacc tggtggctga cctggtacaa gaggttcagc aggcccgtga 
     1741 cctccagaac aggagtgggg ccatgtcccc gatgtcatgg aactcagacg catctacctc 
     1801 cgaagcgtcc tctagttatc cgtacgacgt accagactac gcataagaaa agtgaggatc 
     1861 ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa 
     1921 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc 
     1981 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac 
     2041 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct 
     2101 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc 
     2161 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt 
     2221 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct 
     2281 ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct 
     2341 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac 
     2401 cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg 
     2461 tgctggttgt tgtgctgtct catcattttg gcaaagaatt cgagctcggt acccggggat 
     2521 cctctagagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga 
     2581 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 
     2641 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 
     2701 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct 
     2761 gattatgatc ctgcaagcct cgtcgtctgg ccggaccacg ctatctgtgc aaggtccccg 
     2821 gacgcgcgct ccatgagcag agcgcccgcc gccgaggcaa gactcgggcg gcgccctgcc 
     2881 cgtcccacca ggtcaacagg cggtaaccgg cctcttcatc gggaatgcgc gcgaccttca 
     2941 gcatcgccgg catgtcccct ggcggacggg aagtatcagc tcgaccaagc ttggcgagat 
     3001 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata 
     3061 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct 
     3121 ataaccagac cgttcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
     3181 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     3241 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     3301 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     3361 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     3421 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     3481 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     3541 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     3601 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     3661 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     3721 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     3781 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 
     3841 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     3901 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     3961 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     4021 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     4081 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     4141 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     4201 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     4261 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     4321 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     4381 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     4441 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     4501 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     4561 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agggttatca ctcatggtta 
     4621 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     4681 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     4741 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     4801 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     4861 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     4921 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     4981 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     5041 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     5101 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 
     5161 ataaaaatag gcgtatcacg aggccctttc gtc 
//
