ID pEF1-2FKBP-link-hFADD standard; circular DNA; ; 8217 BP. DE LMBP 4610. CC Accession number: LMBP 4610 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 21-MAY-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT intron 704..1642 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT CDS 2462..2479 FT /vntifkey="4" FT /label=GSGGGS\linker FT CDS 2486..3109 FT /vntifkey="4" FT /label=hFADD FT misc_marker 3116..3142 FT /vntifkey="22" FT /label=HA\epitope FT CDS 1760..1801 FT /vntifkey="4" FT /label=v-src\myristoylation-targeting\sequence FT CDS 1808..2128 FT /vntifkey="4" FT /label=hFKBP1A FT CDS 2135..2455 FT /vntifkey="4" FT /label=hFKBP1A FT misc_marker 3848..3889 FT /vntifkey="22" FT /label=V5\epitope FT misc_marker 3899..3916 FT /vntifkey="22" FT /label=His-tag FT terminator 3945..4169 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 4215..4643 FT /vntifkey="33" FT /label=f1\ori FT rep_origin 4886..4961 FT /vntifkey="33" FT /label=SV40\ori FT terminator 6023..6097 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(6023..6097) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS 5053..5847 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT rep_origin complement(6411..6802) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7343..8203) FT /vntifkey="4" FT /label=ampicillin FT promoter complement(6236..6319) FT /vntifkey="30" FT /label=lac\promoter FT promoter 4648..4929 FT /vntifkey="29" FT /label=SV40\early\promoter SQ Sequence 8217 BP; 1816 A; 2112 C; 2225 G; 2064 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 1740 tgtggtggaa ttcgcgcgta tggggagtag caagagcaag cctaaggacc ccagccagcg 1800 ctctagagga gtgcaggtgg aaaccatctc cccgggagac gggcgcacct tccccaagcg 1860 cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc 1920 ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg 1980 ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc 2040 agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt 2100 cttcgatgtg gagcttctaa aactggaagt cgagggcgtg caggtggaaa ccatctcccc 2160 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat 2220 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat 2280 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg 2340 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg 2400 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactag 2460 agggtctgga ggcggatcct ctagtatgga cccgttcctg gtgctgctgc actcggtgtc 2520 gtccagcctg tcgagcagcg agctgaccga gctcaagttc ctatgcctcg ggcgcgtggg 2580 caagcgcaag ctggagcgcg tgcagagcgg cctagacctc ttctccatgc tgctggagca 2640 gaacgacctg gagcccgggc acaccgagct cctgcgcgag ctgctcgcct ccctgcggcg 2700 ccacgacctg ctgcggcgcg tcgacgactt cgaggcgggg gcggcggccg gggccgcgcc 2760 tggggaagaa gacctgtgtg cagcatttaa cgtcatatgt gataatgtgg ggaaagattg 2820 gagaaggctg gctcgtcagc tcaaagtctc agacaccaag atcgacagca tcgaggacag 2880 atacccccgc aacctgacag agcgtgtgcg ggagtcactg agaatctgga agaacacaga 2940 gaaggagaac gcaacagtgg cccacctggt gggggctctc aggtcctgcc agatgaacct 3000 ggtggctgac ctggtacaag aggttcagca ggcccgtgac ctccagaaca ggagtggggc 3060 catgtccccg atgtcatgga actcagacgc atctacctcc gaagcgtcct ctagttatcc 3120 gtacgacgta ccagactacg cataagaaaa gtgaggatcc tgagaacttc agggtgagtt 3180 tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg 3240 gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct 3300 catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt 3360 tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta 3420 aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag 3480 gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta 3540 aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg 3600 gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata 3660 cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg 3720 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc 3780 atcattttgg caaagaattc tgcagatatc cagcacagtg gcggccgctc gaggtcaccc 3840 attcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca 3900 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 3960 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 4020 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 4080 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 4140 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 4200 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 4260 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 4320 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 4380 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 4440 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 4500 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 4560 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 4620 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 4680 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 4740 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4800 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 4860 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 4920 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 4980 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 5040 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 5100 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 5160 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 5220 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 5280 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 5340 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 5400 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 5460 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 5520 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 5580 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 5640 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 5700 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 5760 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 5820 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca 5880 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 5940 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 6000 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 6060 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6120 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 6180 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6240 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6300 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6360 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6420 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6480 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6540 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6600 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 6660 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 6720 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 6780 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 6840 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6900 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6960 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7020 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7080 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7140 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7200 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7260 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 7320 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 7380 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 7440 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 7500 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 7560 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 7620 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 7680 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7740 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 7800 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 7860 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 7920 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 7980 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 8040 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 8100 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8160 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttc 8217 //