LOCUS       pEF1-2FKBP-link-hFADD-DN        7980 bp    DNA     circular     23-JAN-2009
DEFINITION  LMBP 4611.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4611
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-JAN-2009
            
COMMENT     VNTDATE|475857298|
COMMENT     VNTNAME|pEF1-2FKBP-link-hFADD-DN|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1-2FKBP-link-hFADD-DN" 0 7980 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AdeI" "Alw44I" "BamHI" "Eco81I" "EcoRI" "NdeI" "PdmI" 
COMMENT         "PvuII" "SalI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7980 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "AdeI" "cacnnngtg" 7 0 3 1510 0 2732 0 4212 0 0 
COMMENT            "") 
COMMENT        #4=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 5 155 0 1878 0 2205 0 
COMMENT            6601 0 7847 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 1726 0 2475 0 2919 0 0 
COMMENT            "") #6=(CRSite (CStringList) "Eco81I" "cctnagg" 3 0 1 1783 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1749 0 3559 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 2559 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 3 773 0 4407 0 7779 0 0 
COMMENT            "") 
COMMENT        #10=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 3955 0 5051 0 6122 0 0 
COMMENT             "") 
COMMENT        #11=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 121 0 5924 0 0 "")) 
COMMENT       (CObList 
COMMENT        #12=(CFSignal (CObList) "hEF1 promoter" 29 0 0 508 1651 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "508..1651") 
COMMENT        #13=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1668..1684") 
COMMENT        #14=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 704 1642 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "704..1642") 
COMMENT        #15=(CFSignal (CObList) "hFADD-DN" 4 0 0 2486 2872 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2486..2872") 
COMMENT        #16=(CFSignal (CObList) "HA epitope" 22 0 0 2879 2905 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2879..2905") 
COMMENT        #17=(CFSignal (CObList) "hFKBP1A" 4 0 0 1808 2128 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1808..2128") 
COMMENT        #18=(CFSignal (CObList) "hFKBP1A" 4 0 0 2135 2455 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2135..2455") 
COMMENT        #19=(CFSignal (CObList) "V5 epitope" 22 0 0 3611 3652 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3611..3652") 
COMMENT        #20=(CFSignal (CObList) "His-tag" 22 0 0 3662 3679 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3662..3679") 
COMMENT        #21=(CFSignal (CObList) "BGH polyA" 43 0 0 3708 3932 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3708..3932") 
COMMENT        #22=(CFSignal (CObList) "f1 ori" 33 0 0 3978 4406 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3978..4406") 
COMMENT        #23=(CFSignal (CObList) "SV40 ori" 33 0 0 4649 4724 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4649..4724") 
COMMENT        #24=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5786 5860 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5786..5860") 
COMMENT        #25=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5786 5860 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5786..5860)") 
COMMENT        #26=(CFSignal (CObList) "pMB1 ori" 33 0 1 6174 6565 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6174..6565)") 
COMMENT        #27=(CFSignal (CObList) "lac promoter" 30 0 1 5999 6082 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #28=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4411 4692 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2601..2882") 
COMMENT        #29=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7106 7966 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(7106..7966)") 
COMMENT        #30=(CFSignal (CObList) "v-src myristoylation-targeting sequence" 4 0 0 
COMMENT             1760 1801 0 (CStringList) (CStringList) 1 1 1 1 "1760..1801") 
COMMENT        #31=(CFSignal (CObList) "GSGGGS linker" 4 0 0 2462 2479 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2462..2479") 
COMMENT        #32=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4816 5610 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4816..5610")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #33=(CFSignal (CObList) "LMBP 4611" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #34=(CFSignal (CObList) "Last modified: 23-JAN-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pEF1-2FKBP-link-hFADD-DN" 2) 5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA Plasmid 'pEF1-2FKBP-link-hFADD-DN'" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4611" 
COMMENT                          1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/03/08 02:34" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/23/09 11:57" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7980 bp" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #46=(CFolderPar 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 11-APR-2008" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #49=(CFolderPar 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Author") 
COMMENT              #52=(CFolderPar 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 4611") 
COMMENT                     #59=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #60=(CLinePar (CParagraph 0 (15 #34# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 23-JAN-2009"))) 
COMMENT                   "Annotations") 
COMMENT              #61=(CFolderPar 
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COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1760  End: 1801" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1760..1801" 1))) 
COMMENT                                 "v-src myristoylation-targeting sequence") 
COMMENT                            #67=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1808  End: 2128" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1808..2128" 1))) "hFKBP1A") 
COMMENT                            #71=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 2135  End: 2455" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2135..2455" 1))) "hFKBP1A") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2462 (3 #31# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2462  End: 2479" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2462..2479" 1))) "GSGGGS linker") 
COMMENT                            #79=(CFolderPar 
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COMMENT                                   #80=(CLinePar 
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COMMENT                                        "Start: 2486  End: 2872" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2486..2872" 1))) "hFADD-DN") 
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COMMENT                                        "Start: 4816  End: 5610" 1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                        "   4816..5610" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
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COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7106  End: 7966 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7106..7966)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (7 total)") 
COMMENT                     #91=(CFolderPar 
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COMMENT                                   #94=(CLinePar 
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COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   704..1642" 1))) 
COMMENT                                 "intron hEF1 promoter"))) "Intron (1 total)") 
COMMENT                     #96=(CFolderPar 
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COMMENT                                   #99=(CLinePar 
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COMMENT                                   #100=(CLinePar 
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COMMENT                                         "   2879..2905" 1))) "HA epitope") 
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COMMENT                                    #103=(CLinePar 
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COMMENT                                    #104=(CLinePar 
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COMMENT                                          "   3611..3652" 1))) "V5 epitope") 
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COMMENT                                    #108=(CLinePar 
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COMMENT                                          "   3662..3679" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (3 total)") 
COMMENT                     #109=(CFolderPar 
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COMMENT                                           "Start: 508   End: 1651" 1) 
COMMENT                                     #112=(CLinePar 
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COMMENT                                     #113=(CLinePar 
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COMMENT                                           "   508..1651" 1))) "hEF1 promoter") 
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COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2601..2882" 1))) 
COMMENT                                   "SV40 early promoter"))) 
COMMENT                           "Promoter Eukaryotic (2 total)") 
COMMENT                     #118=(CFolderPar 
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FEATURES             Location/Qualifiers
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     intron          704..1642
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     CDS             2462..2479
                     /vntifkey="4"
                     /label=GSGGGS\linker
     CDS             2486..2872
                     /vntifkey="4"
                     /label=hFADD-DN
     misc_marker     2879..2905
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             1760..1801
                     /vntifkey="4"
                     /label=v-src\myristoylation-targeting\sequence
     CDS             1808..2128
                     /vntifkey="4"
                     /label=hFKBP1A
     CDS             2135..2455
                     /vntifkey="4"
                     /label=hFKBP1A
     misc_marker     3611..3652
                     /vntifkey="22"
                     /label=V5\epitope
     misc_marker     3662..3679
                     /vntifkey="22"
                     /label=His-tag
     terminator      3708..3932
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3978..4406
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      4649..4724
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      5786..5860
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5786..5860)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             4816..5610
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      complement(6174..6565)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7106..7966)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(5999..6082)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        4411..4692
                     /vntifkey="29"
                     /label=SV40\early\promoter
BASE COUNT     1782 a      2028 c      2147 g      2023 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 
     1741 tgtggtggaa ttcgcgcgta tggggagtag caagagcaag cctaaggacc ccagccagcg 
     1801 ctctagagga gtgcaggtgg aaaccatctc cccgggagac gggcgcacct tccccaagcg 
     1861 cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc 
     1921 ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg 
     1981 ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc 
     2041 agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt 
     2101 cttcgatgtg gagcttctaa aactggaagt cgagggcgtg caggtggaaa ccatctcccc 
     2161 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat 
     2221 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat 
     2281 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg 
     2341 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg 
     2401 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactag 
     2461 agggtctgga ggcggatcct ctagtgacga cttcgaggcg ggggcggcgg ccggggccgc 
     2521 gcctggggaa gaagacctgt gtgcagcatt taacgtcata tgtgataatg tggggaaaga 
     2581 ttggagaagg ctggctcgtc agctcaaagt ctcagacacc aagatcgaca gcatcgagga 
     2641 cagatacccc cgcaacctga cagagcgtgt gcgggagtca ctgagaatct ggaagaacac 
     2701 agagaaggag aacgcaacag tggcccacct ggtgggggct ctcaggtcct gccagatgaa 
     2761 cctggtggct gacctggtac aagaggttca gcaggcccgt gacctccaga acaggagtgg 
     2821 ggccatgtcc ccgatgtcat ggaactcaga cgcatctacc tccgaagcgt cctctagtta 
     2881 tccgtacgac gtaccagact acgcataaga aaagtgagga tcctgagaac ttcagggtga 
     2941 gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag 
     3001 ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac 
     3061 cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta 
     3121 ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg taacgaattt 
     3181 ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc 
     3241 aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa 
     3301 ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta 
     3361 ttggtagaaa caactacatc ctggtcatca tcctgccttt ctctttatgg ttacaatgat 
     3421 atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc 
     3481 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt 
     3541 ctcatcattt tggcaaagaa ttctgcagat atccagcaca gtggcggccg ctcgaggtca 
     3601 cccattcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg 
     3661 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag 
     3721 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 
     3781 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 
     3841 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 
     3901 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 
     3961 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     4021 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     4081 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     4141 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     4201 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     4261 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     4321 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     4381 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 
     4441 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     4501 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 
     4561 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 
     4621 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     4681 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 
     4741 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga 
     4801 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 
     4861 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 
     4921 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 
     4981 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 
     5041 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 
     5101 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 
     5161 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 
     5221 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 
     5281 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 
     5341 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 
     5401 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 
     5461 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 
     5521 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 
     5581 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga 
     5641 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 
     5701 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 
     5761 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 
     5821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     5881 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 
     5941 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     6001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
     6061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     6121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     6181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     6241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     6301 gtgagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     6361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     6421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     6481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     6541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     6601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     6661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     6721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     6781 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     6841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     6901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     6961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     7021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     7081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     7141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     7201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     7261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     7321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     7381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     7441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     7501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     7561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     7621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     7681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     7741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     7801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     7861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     7921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
//
