LOCUS       pUHD-2FKBP-link-mCASP-8 6003 bp dsDNACircular
DEFINITION  LMBP 4618.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        4..369
                     /label=hCMVT\promoter
     misc_feature    1163..1180
                     /label=GSGGGS\linker
     CDS             1181..2620
                     /label=mCASP-8
     misc_difference 2627..2653
                     /label=HA\epitope
     CDS             509..829
                     /label=hFKBP12
     CDS             836..1156
                     /label=hFKBP12
     misc_difference 461..502
                     /label=myristoylation-targeting\sequence
     terminator      3400..3496
                     /label=SV40\polyA\late
     terminator      complement(3400..3496)
                     /label=SV40\polyA\early
     rep_origin      complement(4000..4402)
                     /label=pMB1\ori
     CDS             complement(4943..5803)
                     /label=AMP
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc 
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa 
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc 
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag 
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag 
      301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt 
      361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca 
      421 ccgggaccga tccagcctcc gcggccccga attcgcgcgt atggggagta gcaagagcaa 
      481 gcctaaggac cccagccagc gctctagagg agtgcaggtg gaaaccatct ccccgggaga 
      541 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga 
      601 agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg 
      661 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag 
      721 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat 
      781 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaag tcgagggcgt 
      841 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg 
      901 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag 
      961 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg 
     1021 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta 
     1081 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga 
     1141 gcttctaaaa ctggaaacta gtgggtctgg aggcggatcc atggatttcc agagttgtct 
     1201 ttatgctatt gctgaagaac tgggcagtga agacctggct gccctcaagt tcctgtgctt 
     1261 ggactacatc ccacacaaga agcaggagac catcgaggat gcccagaagc tatttctgag 
     1321 gcttcgggaa aaggggatgt tggaggaagg caatctgtct ttcctgaaag agctgctttt 
     1381 ccacatcagt cggtgggacc tgctggtcaa cttcctagac tgcaaccgag aggagatggt 
     1441 gagagagctg cgggacccag acaatgtcca gatttctccc tacagggtca tgctctttaa 
     1501 gctctcagaa gaagtgagcg agttggaatt gagatctttt aagttccttt tgaacaatga 
     1561 gatccccaaa tgtaagctgg aagatgactt gagcctgctt gaaatttttg tagaaatgga 
     1621 gaagaggacc atgctggcag aaaataactt ggaaacccta aaatcaatct gtgaccaggt 
     1681 caacaagagc ctgctgggga agatcgagga ttatgaaaga tcaagcacag agagaagaat 
     1741 gagtcttgaa ggaagggaag agttgccacc ttcagttttg gatgagatga gcctcaaaat 
     1801 ggcggaactg tgtgactcgc caagagaaca agacagtgag tcacggactt cagacaaagt 
     1861 ttaccaaatg aagaacaaac ctcggggata ctgtctgatc atcaacaatc atgatttcag 
     1921 caaggcccgg gaagacataa cccaactccg aaaaatgaag gacagaaaag gaacagactg 
     1981 tgataaagag gctctgagta agacctttaa ggagcttcat tttgagatag tatcttacga 
     2041 cgactgcact gcaaatgaaa tccacgagat tctagaaggc taccaaagcg cagaccacaa 
     2101 gaacaaagac tgcttcatct gctgtatctt atcccacggt gacaagggtg tcgtctatgg 
     2161 aacggatggg aaggaggcct ccatctatga cctgacatct tacttcactg gttcaaagtg 
     2221 cccttccctg tctgggaaac ccaagatctt tttcattcag gcttgccaag gaagtaactt 
     2281 ccagaaagga gtgcctgatg aggcaggctt cgagcaacag aaccacactt tagaagtgga 
     2341 ttcatcatct cacaagaact atattccgga tgaggcagac tttctgctgg gaatggctac 
     2401 ggtgaagaac tgcgtttcct accgagatcc tgtgaatgga acctggtata ttcagtcact 
     2461 ttgccagagc ctgagggaaa gatgtcctca aggagatgac attcttagca tcctgactgg 
     2521 cgtgaactat gacgtgagca ataaagacga caggaggaac aagggaaagc agatgccaca 
     2581 gcccaccttc acactacgga agaagctctt cttccctccc actagttatc cgtacgacgt 
     2641 accagactac gcataagaaa agtgaggatc ctgagaactt cagggtgagt ttggggaccc 
     2701 ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt 
     2761 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat 
     2821 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat 
     2881 tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt 
     2941 ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg 
     3001 gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag 
     3061 gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca 
     3121 actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg 
     3181 agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct 
     3241 tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg 
     3301 gcaaagaatt cgagctcggt acccggggat cctctagagg atccagacat gataagatac 
     3361 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 
     3421 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 
     3481 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc 
     3541 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctgcaagcct cgtcgtctgg 
     3601 ccggaccacg ctatctgtgc aaggtccccg gacgcgcgct ccatgagcag agcgcccgcc 
     3661 gccgaggcaa gactcgggcg gcgccctgcc cgtcccacca ggtcaacagg cggtaaccgg 
     3721 cctcttcatc gggaatgcgc gcgaccttca gcatcgccgg catgtcccct ggcggacggg 
     3781 aagtatcagc tcgaccaagc ttggcgagat tttcaggagc taaggaagct aaaatggaga 
     3841 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 
     3901 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg cattaatgaa 
     3961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     4021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     4081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     4141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     4201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     4261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     4321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     4381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     4441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     4501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     4561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     4621 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     4681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     4741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     4801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     4861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     4921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     4981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     5041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     5101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     5161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     5221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     5281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     5341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     5401 agttggccgc agggttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     5461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     5521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     5581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     5641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     5701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     5761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     5821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     5881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 
     5941 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 
     6001 gtc 
//
