ID pSV23SmTNFd1-9K11E standard; circular DNA; ; 5061 BP. DE LMBP 4638. CC Accession number: LMBP 4638 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 20-DEC-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 1..809 FT /vntifkey="4" FT /label=part\of\SV40\VP1 FT /note="part of the SV40 VP1 coding sequence" FT terminator complement(810..906) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator 810..906 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT misc_feature complement(907..1458) FT /vntifkey="21" FT /label=3'\UTR\of\SV40\small\t-antigen FT /note="3' UTR of the SV40 small t-antigen" FT intron complement(1459..1525) FT /vntifkey="15" FT /label=intron\of\SV40\small\t-antigen FT /note="intron of the SV40 small t-antigen" FT CDS complement(1526..1600) FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT /note="part of the SV40 small t-antigen" FT misc_feature complement(1624..1781) FT /vntifkey="21" FT /label=3'\UTR\of\mTNF FT /note="3' UTR of the mouse tumor necrosis factor gene" FT CDS complement(1782..2225) FT /vntifkey="4" FT /label=mature\mTNFd1-9K11E FT /note="mouse TNF mature sequence with deletion + mutation" FT CDS complement(2226..2462) FT /vntifkey="4" FT /label=presequence\of\mTNF FT /note="mouse tumor necrosis factor presequence" FT misc_feature complement(2463..2490) FT /vntifkey="21" FT /label=5'\UTR\of\mTNF FT /note="5' UTR of the mouse tumor necrosis factor gene" FT promoter complement(2547..2828) FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin complement(2515..2590) FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT rep_origin complement(3050..3452) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3993..4853) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 5061 BP; 1321 A; 1154 C; 1268 G; 1318 t; ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 60 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 120 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 180 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 240 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 300 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 360 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 420 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 480 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 540 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 600 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 660 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 720 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt 780 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 840 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 900 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc 960 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc 1020 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt 1080 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact 1140 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc 1200 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc 1260 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt 1320 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat 1380 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc 1440 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta 1500 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa 1560 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgaccgg gggggggggg 1620 ggggttgaaa gctctgagca cagagttgga ctctgagcca taatcccctt tctaagttag 1680 aaggacacag actgggggct ctgaggagta gacaataaag gggtcagagt aaaggggtca 1740 gagtgggggc tgggtagaga atggatgaac acccattccc ttcacagagc aatgactcca 1800 aagtagacct gcccggactc cgcaaagtct aagtacttgg gcagattgac ctcagcgctg 1860 agttggtccc ccttctccag ctggaagact cctcccaggt atatgggctc ataccagggt 1920 ttgagctcag ccccctcagg ggtgtccttg gggcaggggc tcttgacggc agagaggagg 1980 ttgactttct cctggtatga gatagcaaat cggctgacgg tgtgggtgag gagcacgtag 2040 tcggggcagc cttgtccctt gaagagaacc tgggagtaga caaggtacaa cccatcggct 2100 ggcaccacta gttggttgtc tttgagatcc atgccgttgg ccaggagggc gttggcgcgc 2160 tggctcagcc actccagctg ctcctccact tggtggtttg ctacgacgtg cgcaacaggc 2220 tcgtctgtga gggtctgggc catagaactg atgagaggga ggccatttgg gaacttctca 2280 tccctttggg gaccgatcac cccgaagttc agtagacaga agagcgtggt ggcccctgcc 2340 acaagcagga atgagaagag gctgagacat aggcaccgcc tggagttctg gaagcccccc 2400 atcttttggg ggagtgcctc ttctgccagt tccacgtcgc ggatcatgct ttctgtgctc 2460 atggtgtctt ttctggaggg agatgtggcg ggtcgacgga tcccccctcc aaaaaagcct 2520 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa 2580 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg 2640 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt 2700 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg 2760 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca 2820 ttccacagtc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt 2880 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac 2940 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa 3000 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct 3060 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3120 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3180 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3240 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3300 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3360 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 3420 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 3480 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 3540 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3600 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3660 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3720 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3780 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3840 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 3900 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 3960 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 4020 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 4080 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 4140 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 4200 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 4260 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 4320 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 4380 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 4440 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 4500 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 4560 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 4620 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 4680 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4740 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4800 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4860 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 4920 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 4980 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 5040 aggccctttc gtcttcaaga a 5061 //