ID pGal4-p65S276A standard; circular DNA; ; 6986 BP. DE LMBP 4647. CC Accession number: LMBP 4647 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 21-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 1..825 FT /vntifkey="4" FT /label=part\of\SV40\VP1 FT /note="part of the SV40 VP1 coding sequence" FT terminator 826..900 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator complement(826..900) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT misc_feature complement(934..1723) FT /vntifkey="21" FT /label=3'\UTR\of\hNF-kappaB\p65\subunit FT /note="3' UTR of the human nuclear factor kappaB p65 gene" FT CDS complement(1724..3379) FT /vntifkey="4" FT /label=hNF-kappaB\p65\mutant FT /note="mutated human nuclear factor kappaB p65 coding sequence" FT CDS complement(3380..3415) FT /vntifkey="4" FT /label=linker FT /note="linker" FT CDS complement(3416..3853) FT /vntifkey="4" FT /label=GALbd FT /note="S. cerevisiae GAL4 DNA-binding domain" FT promoter complement(3997..4178) FT /vntifkey="29" FT /label=RSV-LTR FT /note="Rous sarcoma virus long terminal repeat" FT promoter 4575..4856 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 4813..4888 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT rep_origin complement(4975..5377) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5918..6778) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT mutation complement(2552..2557) FT /vntifkey="62" FT /label=S276A FT /note="WT: CCT.TCC" SQ Sequence 6986 BP; 1703 A; 1739 C; 1830 G; 1714 t; ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 60 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 120 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 180 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 240 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 300 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 360 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 420 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 480 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 540 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 600 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 660 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 720 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata 780 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 840 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 900 aattccgcca cggatcaagc ttgatatcga attccttttt tttttttttt cagcttggca 960 acagctttat tagttcagag tagaaagagc aagagtccaa gtgctttgat tgttcactaa 1020 aaactatgcc tcctgactgg agagctgcca gcctgctctc ccccactctt aacaacttac 1080 cctactatta aggcacttga gaagagggga gagcaaggaa gtccagacca aaccccttct 1140 ggatcctgga gagagccagt gctgttgcac tgtttccttc agccatggtt gagcaaggaa 1200 agagccggca gagacctctg tagggcagga aggcagcccc tcaaacgctg gtgttaggca 1260 cagggacaat gccagtgcca tacaggggct ggtatctggg gcgttatttt gattaagctg 1320 taatgaatcc atgatggaag acacttgata aggctttgtg ggctcaaagg ccttacctcc 1380 agcctgcttc tgtctctagg agagtaccag aagctggagg atggggatgg gggaccccac 1440 agttccctac agagaaggga gctgaccatc aggacagggg aaaagtttga gtttccccag 1500 ctcccccctt tccagagaag ttaatgcttc tgcttaagca cctccaaaaa gagagagaga 1560 tacagatact gacaataaaa gaataaaata tggctccccc ctccaaggaa gacatccaca 1620 aagttggggg cagttggaac acaccccacc agaatccgta agtgcttttg gagggcttca 1680 atcccctgca accagtgctc tggggagggc aggcgtcacc cccttaggag ctgatctgac 1740 tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa gtcttcatct cctgaaagga 1800 ggccattggg gagccccggg gcccccagtg gagcaggagc tgggtcgggg ggcctctggg 1860 cccctgtcac taggcgagtt atagcctcag ggtactccat cagcatgggc tcagttgtgt 1920 ggggggccac aggtatgccc tggttcagca gctgctgaaa ctcggagttg tcgacggatg 1980 ccaggtctgt gaacacagct gggtctgtgc tgttgccaag caaggccccc aggtcttcat 2040 catcaaactg cagctgcagc agggcctctg acagcgttcc ttccccagcc tgggtgggct 2100 tgggggcagg tggggccaca gcctgaggag ggcctggggc taggactggg acaggggctg 2160 gggcctgggc cagagctgat accatggctg gagcaggggc aggggctgga gcctggggca 2220 ggacttgggg aggggccggg gccaaggccg aggcctggct gatctgccca gaaggaaaca 2280 ccatggtggg aaactcatca tagttgatgg tgctcaggga tgacgtaaag ggatagggct 2340 ggggtgctgg cttggggaca gaagctgagc tgcgggaagg cacagcaatg cgtcgaggtg 2400 gaggccgggg gtcggtgggt ccgctgaaag gactcttctt catgatgctc ttgaaggtct 2460 catatgtcct tttacgtttc tcctcaatcc ggtgacgatc gtctgtatct ggcaggtact 2520 ggaattccat gggctcactg agctcccggt cggccggccg ccgcagctgc atggagacac 2580 gcacaggagc ctgcaggctg gggtctgcgt agggaggggt ccggaacaca atggccactt 2640 gtcggtgcac atcagcttgc gaaaaggagc ctcgggcctc ccagcctggt cccgtgaaat 2700 acacctcaat gtcctctttc tgcaccttgt cacacagtag gaagatctca tccccaccga 2760 ggcagctgcc agagtttcgg ttcactcggc agatcttgag ctcggcagtg ttgggggcac 2820 gattgtcaaa gatgggatga ggaaggacag gcggcaggcg gaggggcctg cctgatgggt 2880 cccgcactgt cacctggaag cagagccgca cagcattcag gtcgtagtcc ccacgctgct 2940 cttctatagg aacttggaag gggttgttgt tggtctggat gcgctgactg atagcctgct 3000 ccaggtcccg cttcttcaca cactggattc ccaggttctg gaaactgtgg atgcagcggt 3060 ccgggcagag ctcagcctca tagaagccat cccggcagtc ctttcctaca agctcgtggg 3120 ggtgaggccg gtgaggaggg tccttggtga ccagggagat gcgcactgtc cctggtcctg 3180 tgtagccatt gatcttgatg gtggggtggg tcttggtggt atctgtgctc ctctcgcctg 3240 ggatgctgcc cgcggagcgc ccctcgcact tgtagcggaa gcgcatgccc cgctgcttgg 3300 gctgctcaat gatctccaca taggggccag aggcctgggc tggctctgcc gggaagatga 3360 gggggaacag ttcgtccatg gccggggtcc cctggtcgac acccgggaat tccggcgata 3420 cagtcaactg tctttgacct ttgttactac tctcttccga tgatgatgtc gcacttattc 3480 tatgctgtct caatgttaga ggcatatcag tctccactga agccaatcta tctgtgacgg 3540 catctttatt cacattatct tgtacaaata atcctgttaa caatgctttt atatcctgta 3600 aagaatccat tttcaaaatc atgtcaaggt cttctcgagg aaaaatcagt agaaatagct 3660 gttccagtct ttctagcctt gattccactt ctgtcagatg tgccctagtc agcggagacc 3720 ttttggtttt gggagagtag cgacactccc agttgttctt cagacacttg gcgcacttcg 3780 gtttttcttt ggagcacttg agctttttaa gtcggcaaat atcgcatgct tgttcgatag 3840 aagacagtag cttcagatct ggctgctggg agtccatcag tgaatatcaa ctctggcaga 3900 ggagccgctc gcccgccacc gtccgcagtt cccgccgcag ccgagataaa caacttagct 3960 tgtgaacgca gaaggagcag gaggtaccca gcttggaggt gcacaccaat gtggtgaatg 4020 gtcaaatggc gtttattgta tcgagctagg cacttaaata caatatctct gcaatgcgga 4080 attcagtggt tcgtccaatc catgtcagac ctgtctgttg ccttcctaat aaggcacgat 4140 cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat 4200 gttgctaact catcgttacc atgttgcaag actacaagtg tattgcataa gactacattt 4260 ccccctccct atgcaaaagc gaaactacta tatcctgagg ggactcctaa ccgcgtacaa 4320 ccgaagcccc gcttttcgcc taaacacacc ctagtcccct cagatacgcg tatatctggc 4380 ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc 4440 ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa 4500 cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag 4560 agtgcaccat atggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 4620 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 4680 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 4740 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4800 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 4860 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 4920 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 4980 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5040 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5100 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5160 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 5220 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 5280 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 5340 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 5400 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 5460 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 5520 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 5580 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 5640 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 5700 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 5760 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 5820 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 5880 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 5940 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6000 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6060 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6120 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6180 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 6240 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6300 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 6360 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6420 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6480 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg 6540 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 6600 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 6660 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 6720 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 6780 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 6840 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 6900 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 6960 tcacgaggcc ctttcgtctt caagaa 6986 //