ID pdcDNA-FLAG standard; circular DNA; ; 7034 BP. DE LMBP 4704. CC Accession number: LMBP 4704 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 30-SEP-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(5830..6690) FT /vntifkey="4" FT /label=ampicillin\resistance FT misc_marker 696..719 FT /vntifkey="22" FT /label=FLAG\epitope FT attenuator complement(729..853) FT /vntifkey="0" FT /label=attR1 FT attenuator 2309..2433 FT /vntifkey="0" FT /label=attR2 FT CDS 962..1621 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 1963..2268 FT /vntifkey="4" FT /label=ccdB FT promoter 27..610 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT promoter 655..671 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(2453..2469) FT /vntifkey="30" FT /label=SP6\promoter FT terminator 2495..2719 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 2765..3193 FT /vntifkey="33" FT /label=f1\ori FT rep_origin 3364..3439 FT /vntifkey="33" FT /label=SV40\ori FT CDS 3531..4325 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT terminator 4499..4573 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4499..4573) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter complement(4712..4795) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(4887..5289) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 3198..3407 FT /vntifkey="29" FT /label=SV40\early\promoter FT promoter 861..913 FT /vntifkey="30" FT /label=lacUV5\promoter SQ Sequence 7034 BP; 1710 A; 1732 C; 1758 G; 1834 t; cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 60 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 120 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 180 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg 240 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 300 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 360 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 420 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 480 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 540 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 600 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 660 gactcactat agggagaccc aagcttacca ccatggacac taaggatgac gatgacaaag 720 cggccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca 780 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 840 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc 900 gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa 960 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 1020 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 1080 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 1140 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 1200 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 1260 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 1320 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 1380 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 1440 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 1500 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca 1560 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 1620 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt 1680 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct 1740 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat 1800 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc 1860 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg 1920 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac 1980 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac 2040 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc 2100 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc 2160 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc 2220 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc 2280 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta 2340 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta 2400 cgtttctcgt tcagctttct tgtacaaagt ggtgatctag agggccctat tctatagtgt 2460 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 2520 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 2580 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 2640 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 2700 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 2760 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 2820 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 2880 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga 2940 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 3000 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 3060 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 3120 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 3180 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 3240 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 3300 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 3360 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 3420 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 3480 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 3540 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 3600 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 3660 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 3720 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 3780 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 3840 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 3900 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 3960 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 4020 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 4080 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 4140 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 4200 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 4260 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 4320 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 4380 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 4440 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 4500 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 4560 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 4620 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 4680 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 4740 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 4800 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 4860 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 4920 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 4980 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 5040 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5100 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 5160 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5220 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag 5280 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5340 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5400 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5460 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 5520 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5580 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 5640 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 5700 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5760 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5820 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 5880 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 5940 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6000 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6060 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6120 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 6180 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6240 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6300 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6360 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6420 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 6480 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6540 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 6600 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 6660 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6720 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6780 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga 6840 tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc 6900 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa 6960 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt 7020 tttgcgctgc ttcg 7034 //