LOCUS       pGal4-p65f1-285         5393 bp    DNA     circular     23-APR-2008
DEFINITION  LMBP 4713.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4713
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-APR-2008
            
COMMENT     VNTNAME|pGal4-p65f1-285|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGal4-p65f1-285" 0 5393 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AvaI" "BamHI" "BglII" "EcoRI" "HindIII" "KpnI" "PstI" 
COMMENT         "SmaI" "XhoI" "HaeII") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 4339 4338 1 5393 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 1079 0 1810 0 2042 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 3 1151 0 1199 0 2263 0 0 
COMMENT            "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 4 930 0 1815 0 2487 0 5392 
COMMENT            0 0 "") 
COMMENT        #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 918 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2395 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 209 0 1003 0 4644 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1812 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2042 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 3 1668 0 3384 0 3754 0 0 
COMMENT             "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "part of SV40 VP1" 4 0 0 1 825 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..825") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 0 826 900 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "826..900") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA early" 43 0 1 826 900 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(826..900)") 
COMMENT        #16=(CFSignal (CObList) "hNF-kappaB p65 subunit (1-286)" 4 0 1 929 1786 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "complement(929..1786)") 
COMMENT        #17=(CFSignal (CObList) "linker" 4 0 1 1787 1822 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1787..1822)") 
COMMENT        #18=(CFSignal (CObList) "GALbd" 4 0 1 1823 2260 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1823..2260)") 
COMMENT        #19=(CFSignal (CObList) "RSV-LTR" 29 0 1 2404 2585 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2404..2585)") 
COMMENT        #20=(CFSignal (CObList) "SV40 early promoter" 29 0 0 2982 3263 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2982..3263") 
COMMENT        #21=(CFSignal (CObList) "SV40 ori" 33 0 0 3220 3295 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3220..3295") 
COMMENT        #22=(CFSignal (CObList) "pMB1 ori" 33 0 1 3382 3784 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3382..3784)") 
COMMENT        #23=(CFSignal (CObList) "ampicillin" 4 0 1 4325 5185 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4325..5185)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #24=(CFSignal (CObList) "LMBP 4713" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #25=(CFSignal (CObList) "Last modified: 23-APR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    2) 5 "" 0 4) 
COMMENT              #28=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pGal4-p65f1-285'" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4713" 
COMMENT                          1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/23/08 03:21" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5393 bp" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #37=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #39=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #24# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4713") 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 23-APR-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 825 " 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "part of the SV40 VP1 coding sequence" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..825" 1))) "part of SV40 VP1") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 929 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 929   End: 1786 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of the human nuclear factor kappaB p65 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(929..1786)" 1))) 
COMMENT                                 "hNF-kappaB p65 subunit (1-286)") 
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COMMENT                                  (CParagraph 1787 (3 #17# 0) 1 2 2 0 327) 
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COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1787  End: 1822 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "linker" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1787..1822)" 1))) 
COMMENT                                 "linker") 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1823 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1823  End: 2260 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae GAL4 DNA-binding domain" 
COMMENT                                        1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1823..2260)" 1))) "GALbd") 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4325 (3 #23# 0) 1 2 2 0 327) 
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COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4325  End: 5185 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4325..5185)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (5 total)") 
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COMMENT                                  (CObjectList 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2404  End: 2585 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Rous sarcoma virus long terminal repeat" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2404..2585)" 1))) 
COMMENT                                 "RSV-LTR") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2982 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
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COMMENT                                        "Start: 2982  End: 3263" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "SV40 early promoter" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2982..3263" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #83=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3220  End: 3295" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bidirectional origin of SV40" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3220..3295" 1))) "SV40 ori") 
COMMENT                            #89=(CFolderPar 
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COMMENT                                  (CParagraph 3382 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Start: 3382  End: 3784 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #92=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3382..3784)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #94=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 826   End: 900 " 1) 
COMMENT                                   #97=(CLinePar 
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COMMENT                                        "polyA signal of the SV40 late region" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   826..900" 1))) "SV40 polyA late") 
COMMENT                            #100=(CFolderPar 
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COMMENT                                          "Start: 826   End: 900  (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "polyA signal of the SV40 early region" 
COMMENT                                          1) 
COMMENT                                    #103=(CLinePar 
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COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(826..900)" 1))) 
COMMENT                                  "SV40 polyA early"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #105=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #106=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" 
COMMENT                            "CCTAGG") 
COMMENT                      #107=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #109=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 918 " 1))))) "HindIII: 1 site") 
COMMENT                            1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #110=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #112=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2395 " 1))))) "KpnI: 1 site") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #113=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #115=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1812 " 1))))) "SmaI: 1 site") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #116=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #118=(CLinePar 
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FEATURES             Location/Qualifiers
     CDS             1..825
                     /vntifkey="4"
                     /label=part\of\SV40\VP1
                     /note="part of the SV40 VP1 coding sequence"
     terminator      826..900
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      complement(826..900)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     CDS             complement(929..1786)
                     /vntifkey="4"
                     /label=hNF-kappaB\p65\subunit\(1-286)
                     /note="fragment of the human nuclear factor kappaB p65 coding sequence"
     CDS             complement(1787..1822)
                     /vntifkey="4"
                     /label=linker
                     /note="linker"
     CDS             complement(1823..2260)
                     /vntifkey="4"
                     /label=GALbd
                     /note="S. cerevisiae GAL4 DNA-binding domain"
     promoter        complement(2404..2585)
                     /vntifkey="29"
                     /label=RSV-LTR
                     /note="Rous sarcoma virus long terminal repeat"
     promoter        2982..3263
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /note="SV40 early promoter"
     rep_origin      3220..3295
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     rep_origin      complement(3382..3784)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4325..5185)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1342 a      1348 c      1328 g      1375 t 
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata 
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 
      901 aattccgcca cggatcaagc ttgatatcga attccatggg ctcactgagc tcccggtcgg 
      961 aaggccgccg cagctgcatg gagacacgca caggagcctg caggctgggg tctgcgtagg 
     1021 gaggggtccg gaacacaatg gccacttgtc ggtgcacatc agcttgcgaa aaggagcctc 
     1081 gggcctccca gcctggtccc gtgaaataca cctcaatgtc ctctttctgc accttgtcac 
     1141 acagtaggaa gatctcatcc ccaccgaggc agctgccaga gtttcggttc actcggcaga 
     1201 tcttgagctc ggcagtgttg ggggcacgat tgtcaaagat gggatgagga aggacaggcg 
     1261 gcaggcggag gggcctgcct gatgggtccc gcactgtcac ctggaagcag agccgcacag 
     1321 cattcaggtc gtagtcccca cgctgctctt ctataggaac ttggaagggg ttgttgttgg 
     1381 tctggatgcg ctgactgata gcctgctcca ggtcccgctt cttcacacac tggattccca 
     1441 ggttctggaa actgtggatg cagcggtccg ggcagagctc agcctcatag aagccatccc 
     1501 ggcagtcctt tcctacaagc tcgtgggggt gaggccggtg aggagggtcc ttggtgacca 
     1561 gggagatgcg cactgtccct ggtcctgtgt agccattgat cttgatggtg gggtgggtct 
     1621 tggtggtatc tgtgctcctc tcgcctggga tgctgcccgc ggagcgcccc tcgcacttgt 
     1681 agcggaagcg catgccccgc tgcttgggct gctcaatgat ctccacatag gggccagagg 
     1741 cctgggctgg ctctgccggg aagatgaggg ggaacagttc gtccatggcc ggggtcccct 
     1801 ggtcgacacc cgggaattcc ggcgatacag tcaactgtct ttgacctttg ttactactct 
     1861 cttccgatga tgatgtcgca cttattctat gctgtctcaa tgttagaggc atatcagtct 
     1921 ccactgaagc caatctatct gtgacggcat ctttattcac attatcttgt acaaataatc 
     1981 ctgttaacaa tgcttttata tcctgtaaag aatccatttt caaaatcatg tcaaggtctt 
     2041 ctcgaggaaa aatcagtaga aatagctgtt ccagtctttc tagccttgat tccacttctg 
     2101 tcagatgtgc cctagtcagc ggagaccttt tggttttggg agagtagcga cactcccagt 
     2161 tgttcttcag acacttggcg cacttcggtt tttctttgga gcacttgagc tttttaagtc 
     2221 ggcaaatatc gcatgcttgt tcgatagaag acagtagctt cagatctggc tgctgggagt 
     2281 ccatcagtga atatcaactc tggcagagga gccgctcgcc cgccaccgtc cgcagttccc 
     2341 gccgcagccg agataaacaa cttagcttgt gaacgcagaa ggagcaggag gtacccagct 
     2401 tggaggtgca caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg agctaggcac 
     2461 ttaaatacaa tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg 
     2521 tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac 
     2581 ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact 
     2641 acaagtgtat tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat 
     2701 cctgagggga ctcctaaccg cgtacaaccg aagccccgct tttcgcctaa acacacccta 
     2761 gtcccctcag atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac 
     2821 cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt 
     2881 gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta 
     2941 tgcggcatca gagcagattg tactgagagt gcaccatatg gctgtggaat gtgtgtcagt 
     3001 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     3061 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
     3121 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     3181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     3241 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     3301 gaggcctagg cttttgcaaa aagctccata tgcggtgtga aataccgcac agatgcgtaa 
     3361 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     3421 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     3481 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     3541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     3601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     3661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     3721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     3781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     3841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     3901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     3961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     4021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     4081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     4141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     4201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     4261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     4321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     4381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     4441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     4501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     4561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     4621 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     4681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     4741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     4801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     4861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     4921 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 
     4981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     5041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     5101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     5161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     5221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     5281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
     5341 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa 
//
