LOCUS       pEF1-hRIP1              8192 bp    DNA     circular     20-DEC-2007
DEFINITION  LMBP 4717.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4717
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 20-DEC-2007
            
            
            
COMMENT     VNTNAME|pEF1-hRIP1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1-hRIP1" 0 8192 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "BamHI" "NdeI" "NotI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2606 2605 1 8192 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1726 0 3222 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 2466 0 3272 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1776 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3802 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "hEF1 promoter" 29 0 0 508 1651 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "508..1651") 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1668..1684") 
COMMENT        #6=(CFSignal (CObList) "hRIP1" 4 0 0 1785 3800 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1785..3800") 
COMMENT        #7=(CFSignal (CObList) "V5 epitope" 22 0 0 3823 3864 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3823..3864") 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 0 3874 3891 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3874..3891") 
COMMENT        #9=(CFSignal (CObList) "bovine growth hormone polyA" 43 0 0 3920 4144 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3920..4144") 
COMMENT        #10=(CFSignal (CObList) "f1 ori" 33 0 0 4190 4618 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4190..4618") 
COMMENT        #11=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4623 4904 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4623..4904") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 4861 4936 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4861..4936") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5998 6072 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5998..6072") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5998 6072 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5998..6072)") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 6386 6777 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6386..6777)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin" 4 0 1 7318 8178 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7318..8178)") 
COMMENT        #17=(CFSignal (CObList) "neomycin" 4 0 0 5028 5822 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5028..5822") 
COMMENT        #18=(CFSignal (CObList) "intron" 15 0 0 704 1642 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "704..1642")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "LMBP 4717" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "Last modified: 20-DEC-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #21=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #22=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pEF1-hRIP1" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #23=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pEF1-hRIP1'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4717." 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 12/20/07 12:10" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8192 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-NOV-2006" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #35=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #37=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #38=(CLinePar (CParagraph 0 (15 #19# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4717") 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CLinePar (CParagraph 0 (15 #20# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 20-DEC-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #41=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1785 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1785  End: 3800" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human receptor interacting protein 1 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1785..3800" 1))) "hRIP1") 
COMMENT                            #48=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5028 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5028  End: 5822" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Tn5 antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5028..5822" 1))) "neomycin") 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7318 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7318  End: 8178 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7318..8178)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (3 total)") 
COMMENT                     #58=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 704 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 704   End: 1642" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human elongation factor 1alpha intron" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   704..1642" 1))) "intron"))) 
COMMENT                          "Intron (1 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3823 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3823  End: 3864" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "V5 epitope" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3823..3864" 1))) "V5 epitope") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3874 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3874  End: 3891" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyhistidine (6 x His) tag" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3874..3891" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #75=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 508 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 508   End: 1651" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human elongation factor 1alpha promoter" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   508..1651" 1))) "hEF1 promoter") 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4623 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4623  End: 4904" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "SV40 early promoter" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4623..4904" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1668 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1668  End: 1684" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage T7 gene 10 promoter" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1668..1684" 1))) "T7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #92=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4190 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4190  End: 4618" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage f1 origin" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4190..4618" 1))) "f1 ori") 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4861 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4861  End: 4936" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "bidirectional origin of SV40" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   4861..4936" 1))) "SV40 ori") 
COMMENT                            #103=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 6386 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 6386  End: 6777 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "origin plasmid" 1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(6386..6777)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #108=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #109=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3920 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3920  End: 4144" 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the bovine growth hormone" 
COMMENT                                           1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3920..4144" 1))) 
COMMENT                                   "bovine growth hormone polyA") 
COMMENT                             #114=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5998 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5998  End: 6072" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 early region" 
COMMENT                                           1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5998..6072" 1))) 
COMMENT                                   "SV40 polyA early") 
COMMENT                             #119=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5998 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5998  End: 6072 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 late region" 
COMMENT                                           1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(5998..6072)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #124=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #125=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 36809080 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #126=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #127=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1726 " 1) 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3222 " 1))))) "BamHI: 2 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #129=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 36807592 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #130=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #131=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2466 " 1) 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3272 " 1))))) "NdeI: 2 sites") 
COMMENT                            2 4 "CATATG" "GTATAC") 
COMMENT                      #133=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 36458608 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #134=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #135=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1776 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #136=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 36705432 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #137=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3802 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
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FEATURES             Location/Qualifiers
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
                     /note="human elongation factor 1alpha promoter"
     intron          704..1642
                     /vntifkey="15"
                     /label=intron
                     /note="human elongation factor 1alpha intron"
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     CDS             1785..3800
                     /vntifkey="4"
                     /label=hRIP1
                     /note="human receptor interacting protein 1 coding sequence"
     misc_marker     3823..3864
                     /vntifkey="22"
                     /label=V5\epitope
                     /note="V5 epitope"
     misc_marker     3874..3891
                     /vntifkey="22"
                     /label=His-tag
                     /note="polyhistidine (6 x His) tag"
     terminator      3920..4144
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
                     /note="polyA signal of the bovine growth hormone"
     rep_origin      4190..4618
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     promoter        4623..4904
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /note="SV40 early promoter"
     rep_origin      4861..4936
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     CDS             5028..5822
                     /vntifkey="4"
                     /label=neomycin
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     terminator      5998..6072
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(5998..6072)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(6386..6777)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(7318..8178)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1959 a      2056 c      2195 g      1982 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 
     1741 tgtggtggaa ttctgcagat atccagcaca gtggcggccg caccatgcaa ccagacatgt 
     1801 ccttgaatgt cattaagatg aaatccagtg acttcctgga gagtgcagaa ctggacagcg 
     1861 gaggctttgg gaaggtgtct ctgtgtttcc acagaaccca gggactcatg atcatgaaaa 
     1921 cagtgtacaa ggggcccaac tgcattgagc acaacgaggc cctcttggag gaggcgaaga 
     1981 tgatgaacag actgagacac agccgggtgg tgaagctcct gggcgtcatc atagaggaag 
     2041 ggaagtactc cctggtgatg gagtacatgg agaagggcaa cctgatgcac gtgctgaaag 
     2101 ccgagatgag tactccgctt tctgtaaaag gaaggataat tttggaaatc attgaaggaa 
     2161 tgtgctactt acatggaaaa ggcgtgatac acaaggacct gaagcctgaa aatatccttg 
     2221 ttgataatga cttccacatt aagatcgcag acctcggcct tgcctccttt aagatgtgga 
     2281 gcaaactgaa taatgaagag cacaatgagc tgagggaagt ggacggcacc gctaagaaga 
     2341 atggcggcac cctctactac atggcgcccg agcacctgaa tgacgtcaac gcaaagccca 
     2401 cagagaagtc ggatgtgtac agctttgctg tagtactctg ggcgatattt gcaaataagg 
     2461 agccatatga aaatgctatc tgtgagcagc agttgataat gtgcataaaa tctgggaaca 
     2521 ggccagatgt ggatgacatc actgagtact gcccaagaga aattatcagt ctcatgaagc 
     2581 tctgctggga agcgaatccg gaagctcggc cgacatttcc tggcattgaa gaaaaattta 
     2641 ggccttttta tttaagtcaa ttagaagaaa gtgtagaaga ggacgtgaag agtttaaaga 
     2701 aagagtattc aaacgaaaat gcagttgtga agagaatgca gtctcttcaa cttgattgtg 
     2761 tggcagtacc ttcaagccgg tcaaattcag ccacagaaca gcctggttca ctgcacagtt 
     2821 cccagggact tgggatgggt cctgtggagg agtcctggtt tgctccttcc ctggagcacc 
     2881 cacaagaaga gaatgagccc agcctgcaga gtaaactcca agacgaagcc aactaccatc 
     2941 tttatggcag ccgcatggac aggcagacga aacagcagcc cagacagaat gtggcttaca 
     3001 acagagagga ggaaaggaga cgcagggtct cccatgaccc ttttgcacag caaagacctt 
     3061 acgagaattt tcagaataca gagggaaaag gcactgttta ttccagtgca gccagtcatg 
     3121 gtaatgcagt gcaccagccc tcagggctca ccagccaacc tcaagtactg tatcagaaca 
     3181 atggattata tagctcacat ggctttggaa caagaccact ggatccagga acagcaggtc 
     3241 ccagagtttg gtacaggcca attccaagtc atatgcctag tctgcataat atcccagtgc 
     3301 ctgagaccaa ctatctagga aatacaccca ccatgccatt cagctccttg ccaccaacag 
     3361 atgaatctat aaaatatacc atatacaata gtactggcat tcagattgga gcctacaatt 
     3421 atatggagat tggtgggacg agttcatcac tactagacag cacaaatacg aacttcaaag 
     3481 aagagccagc tgctaagtac caagctatct ttgataatac cactagtctg acggataaac 
     3541 acctggaccc aatcagggaa aatctgggaa agcactggaa aaactgtgcc cgtaaactgg 
     3601 gcttcacaca gtctcagatt gatgaaattg accatgacta tgagcgagat ggactgaaag 
     3661 aaaaggttta ccagatgctc caaaagtggg tgatgaggga aggcataaag ggagccacgg 
     3721 tggggaagct ggcccaggcg ctccaccagt gttccaggat cgaccttctg agcagcttga 
     3781 tttacgtcag ccagaactaa tctagagggc ccgcggttcg aaggtaagcc tatccctaac 
     3841 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa 
     3901 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 
     3961 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 
     4021 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 
     4081 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 
     4141 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 
     4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 
     4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 
     4621 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     4681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
     4741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     4801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     4861 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 
     4921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 
     4981 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 
     5041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 
     5101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 
     5161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 
     5221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 
     5281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 
     5341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 
     5401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 
     5461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 
     5521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 
     5581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 
     5641 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 
     5701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 
     5761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 
     5821 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga 
     5881 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 
     5941 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 
     6001 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     6061 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     6121 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 
     6181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     6241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     6301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     6361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     6421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     6481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagga accgtaaaaa 
     6541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     6601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     6661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     6721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     6781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     6841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     6901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     6961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 
     7021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     7081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     7141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     7201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     7261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     7321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     7381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     7441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     7501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     7561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     7621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     7681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     7741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     7801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     7861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     7921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     7981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     8041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     8101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     8161 gaaatgttga atactcatac tcttcctttt tc 
//
