ID pEF1-hRIP1 standard; circular DNA; ; 8192 BP. DE LMBP 4717. CC Accession number: LMBP 4717 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 20-DEC-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT /note="human elongation factor 1alpha promoter" FT intron 704..1642 FT /vntifkey="15" FT /label=intron FT /note="human elongation factor 1alpha intron" FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT CDS 1785..3800 FT /vntifkey="4" FT /label=hRIP1 FT /note="human receptor interacting protein 1 coding sequence" FT misc_marker 3823..3864 FT /vntifkey="22" FT /label=V5\epitope FT /note="V5 epitope" FT misc_marker 3874..3891 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 3920..4144 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT /note="polyA signal of the bovine growth hormone" FT rep_origin 4190..4618 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 4623..4904 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 4861..4936 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT CDS 5028..5822 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT terminator 5998..6072 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(5998..6072) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(6386..6777) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(7318..8178) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 8192 BP; 1959 A; 2056 C; 2195 G; 1982 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 1740 tgtggtggaa ttctgcagat atccagcaca gtggcggccg caccatgcaa ccagacatgt 1800 ccttgaatgt cattaagatg aaatccagtg acttcctgga gagtgcagaa ctggacagcg 1860 gaggctttgg gaaggtgtct ctgtgtttcc acagaaccca gggactcatg atcatgaaaa 1920 cagtgtacaa ggggcccaac tgcattgagc acaacgaggc cctcttggag gaggcgaaga 1980 tgatgaacag actgagacac agccgggtgg tgaagctcct gggcgtcatc atagaggaag 2040 ggaagtactc cctggtgatg gagtacatgg agaagggcaa cctgatgcac gtgctgaaag 2100 ccgagatgag tactccgctt tctgtaaaag gaaggataat tttggaaatc attgaaggaa 2160 tgtgctactt acatggaaaa ggcgtgatac acaaggacct gaagcctgaa aatatccttg 2220 ttgataatga cttccacatt aagatcgcag acctcggcct tgcctccttt aagatgtgga 2280 gcaaactgaa taatgaagag cacaatgagc tgagggaagt ggacggcacc gctaagaaga 2340 atggcggcac cctctactac atggcgcccg agcacctgaa tgacgtcaac gcaaagccca 2400 cagagaagtc ggatgtgtac agctttgctg tagtactctg ggcgatattt gcaaataagg 2460 agccatatga aaatgctatc tgtgagcagc agttgataat gtgcataaaa tctgggaaca 2520 ggccagatgt ggatgacatc actgagtact gcccaagaga aattatcagt ctcatgaagc 2580 tctgctggga agcgaatccg gaagctcggc cgacatttcc tggcattgaa gaaaaattta 2640 ggccttttta tttaagtcaa ttagaagaaa gtgtagaaga ggacgtgaag agtttaaaga 2700 aagagtattc aaacgaaaat gcagttgtga agagaatgca gtctcttcaa cttgattgtg 2760 tggcagtacc ttcaagccgg tcaaattcag ccacagaaca gcctggttca ctgcacagtt 2820 cccagggact tgggatgggt cctgtggagg agtcctggtt tgctccttcc ctggagcacc 2880 cacaagaaga gaatgagccc agcctgcaga gtaaactcca agacgaagcc aactaccatc 2940 tttatggcag ccgcatggac aggcagacga aacagcagcc cagacagaat gtggcttaca 3000 acagagagga ggaaaggaga cgcagggtct cccatgaccc ttttgcacag caaagacctt 3060 acgagaattt tcagaataca gagggaaaag gcactgttta ttccagtgca gccagtcatg 3120 gtaatgcagt gcaccagccc tcagggctca ccagccaacc tcaagtactg tatcagaaca 3180 atggattata tagctcacat ggctttggaa caagaccact ggatccagga acagcaggtc 3240 ccagagtttg gtacaggcca attccaagtc atatgcctag tctgcataat atcccagtgc 3300 ctgagaccaa ctatctagga aatacaccca ccatgccatt cagctccttg ccaccaacag 3360 atgaatctat aaaatatacc atatacaata gtactggcat tcagattgga gcctacaatt 3420 atatggagat tggtgggacg agttcatcac tactagacag cacaaatacg aacttcaaag 3480 aagagccagc tgctaagtac caagctatct ttgataatac cactagtctg acggataaac 3540 acctggaccc aatcagggaa aatctgggaa agcactggaa aaactgtgcc cgtaaactgg 3600 gcttcacaca gtctcagatt gatgaaattg accatgacta tgagcgagat ggactgaaag 3660 aaaaggttta ccagatgctc caaaagtggg tgatgaggga aggcataaag ggagccacgg 3720 tggggaagct ggcccaggcg ctccaccagt gttccaggat cgaccttctg agcagcttga 3780 tttacgtcag ccagaactaa tctagagggc ccgcggttcg aaggtaagcc tatccctaac 3840 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa 3900 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 3960 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 4020 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 4080 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 4140 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 4200 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 4260 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 4320 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 4380 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 4440 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4500 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4560 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 4620 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 4680 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 4740 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 4800 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 4860 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 4920 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 4980 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 5040 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 5100 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 5160 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 5220 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 5280 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 5340 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 5400 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 5460 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 5520 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 5580 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 5640 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 5700 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 5760 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 5820 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga 5880 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 5940 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 6000 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 6060 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 6120 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 6180 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 6240 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 6300 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 6360 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 6420 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6480 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagga accgtaaaaa 6540 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6600 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6660 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6720 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 6780 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 6840 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 6900 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 6960 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 7020 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7080 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7140 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7200 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7260 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 7320 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 7380 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 7440 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 7500 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 7560 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 7620 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 7680 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 7740 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 7800 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 7860 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 7920 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 7980 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8040 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8100 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8160 gaaatgttga atactcatac tcttcctttt tc 8192 //