LOCUS       pEF1-mRIP               8089 bp    DNA     circular     11-MAR-2008
DEFINITION  LMBP 4720.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4720
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2008
            
COMMENT     VNTDATE|476019726|
COMMENT     VNTNAME|pEF1-mRIP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1-mRIP" 0 8089 
COMMENT       (CDBMol 0 0 1 4388 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "HindII" "NcoI" "SacI" "SalI" "SphI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 7233 7232 1 8089 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1726 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglII" "agatct" 2 0 2 135 0 1040 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindII" "gtyrac" 4 0 4 123 0 357 0 3389 0 6035 
COMMENT            0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1845 0 4751 0 5486 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SacI" "gagctc" 6 0 3 1302 0 1724 0 3003 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 121 0 3387 0 6033 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SphI" "gcatgc" 6 0 4 4018 0 4592 0 4664 0 5459 
COMMENT            0 0 "") #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3703 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 6108 6191 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 6283 6674 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4368..4759)") 
COMMENT        #13=(CFSignal (CObList) "ampicillin" 4 0 1 7215 8075 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5300..6160)") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5895 5969 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3980..4054)") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5895 5969 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3980..4054") 
COMMENT        #16=(CFSignal (CObList) "SV40 ori" 33 0 0 4758 4833 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2843..2918") 
COMMENT        #17=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4520 4801 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2605..2886") 
COMMENT        #18=(CFSignal (CObList) "f1 ori" 33 0 0 4087 4515 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2172..2600") 
COMMENT        #19=(CFSignal (CObList) "BGH polyA" 43 0 0 3817 4041 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1902..2126") 
COMMENT        #20=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 704 1642 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "704..1642") 
COMMENT        #21=(CFSignal (CObList) "His-tag" 22 0 0 3771 3788 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1856..1873") 
COMMENT        #22=(CFSignal (CObList) "V5 epitope" 22 0 0 3720 3761 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1805..1846") 
COMMENT        #23=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1668..1684") 
COMMENT        #24=(CFSignal (CObList) "hEF1 promoter" 29 0 0 508 1651 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "508..1651") 
COMMENT        #25=(CFSignal (CObList) "mRIP1" 4 0 0 1731 3701 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1731..3701") 
COMMENT        #26=(CFSignal (CObList) "neomycin" 4 0 0 4925 5719 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4925..5719")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #27=(CFSignal (CObList) "LMBP 4720" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "Last modified: 11-MAR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   "" 0 4) 
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COMMENT                          "DNA Plasmid 'pEF1-mRIP'" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4720." 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 03/05/08 11:42" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/11/08 10:36" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8089 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 5-MAR-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 4720") 
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COMMENT                   "Annotations") 
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COMMENT                     #50=(CFolderPar 
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COMMENT                                        "Tn5 antibiotic resistance gene; coding sequence" 
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COMMENT                                        "   complement(5300..6160)" 1))) 
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COMMENT                                        "Start: 3771  End: 3788" 1) 
COMMENT                                   #76=(CLinePar 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "   1856..1873" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                                   #82=(CLinePar 
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COMMENT                                        "   508..1651" 1))) "hEF1 promoter") 
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COMMENT                                        "Start: 4520  End: 4801" 1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                        "   2605..2886" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #90=(CLinePar 
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COMMENT                                        "   1668..1684" 1))) "T7 promoter") 
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COMMENT                                        "Start: 6108  End: 6191 (Complementary)" 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                        "   2172..2600" 1))) "f1 ori") 
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COMMENT                                          "   complement(4368..4759)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                           "   1902..2126" 1))) "BGH polyA") 
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COMMENT                                     #115=(CLinePar 
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COMMENT                                           "   complement(3980..4054)" 1))) 
COMMENT                                   "SV40 polyA late") 
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COMMENT                                     #119=(CLinePar 
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COMMENT                                           "   3980..4054" 1))) 
COMMENT                                   "SV40 polyA early"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     promoter        complement(6108..6191)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(6283..6674)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             4925..5719
                     /vntifkey="4"
                     /label=neomycin
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     CDS             complement(7215..8075)
                     /vntifkey="4"
                     /label=ampicillin
     terminator      complement(5895..5969)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      5895..5969
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      4758..4833
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        4520..4801
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4087..4515
                     /vntifkey="33"
                     /label=f1\ori
     terminator      3817..4041
                     /vntifkey="43"
                     /label=BGH\polyA
     intron          704..1642
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     misc_marker     3771..3788
                     /vntifkey="22"
                     /label=His-tag
     misc_marker     3720..3761
                     /vntifkey="22"
                     /label=V5\epitope
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
     CDS             1731..3701
                     /vntifkey="4"
                     /label=mRIP1
                     /note="mouse receptor interacting protein 1 coding sequence"
BASE COUNT     1941 a      2022 c      2162 g      1964 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc atgcaaccag 
     1741 acatgtcctt ggacaatatt aagatggcat ccagtgacct gctggagaag acagacctag 
     1801 acagcggagg cttcgggaag gtgtccttgt gttaccacag aagccatgga tttgtcatcc 
     1861 tgaaaaaagt atacacaggg cccaaccgcg ctgagtacaa tgaggttctc ttggaagagg 
     1921 ggaagatgat gcacagactg agacacagtc gagtggtgaa gctactgggc atcatcatag 
     1981 aagaagggaa ctattcgctg gtgatggagt acatggagaa gggcaacctg atgcacgtgc 
     2041 taaagaccca gatagatgtc ccactttcat tgaaaggaag gataatcgtg gaggccatag 
     2101 aaggcatgtg ctacttacat gacaaaggtg tgatacacaa ggacctgaag cctgagaata 
     2161 tcctcgttga tcgtgacttt cacattaaga tagccgatct tggtgtggct tcctttaaga 
     2221 catggagcaa actgactaag gagaaagaca acaagcagaa agaagtgagc agcaccacta 
     2281 agaagaacaa tggtggtacc ctttactaca tggcacccga acacctgaat gacatcaatg 
     2341 caaagcccac ggagaagtcg gacgtgtaca gctttggcat tgtcctttgg gcaatatttg 
     2401 caaaaaagga gccctatgag aatgtcatct gtactgagca gttcgtgatc tgcataaaat 
     2461 ctgggaacag gccaaatgta gaggaaatcc ttgagtactg tccaagggag atcatcagcc 
     2521 tcatggagcg gtgctggcag gcgatcccag aagacaggcc aacatttctt ggcattgaag 
     2581 aagaatttag gcctttttac ttaagtcatt ttgaagaata tgtagaagag gatgtggcaa 
     2641 gtttaaagaa agagtatcca gatcaaagcc cagtgctgca gagaatgttt tcactgcagc 
     2701 atgactgtgt acccttacct ccgagcaggt caaattcaga acaacctgga tcgctgcaca 
     2761 gttcccaggg gctccagatg ggtcctgtgg aggagtcctg gttttcttcc tccccagagt 
     2821 acccacagga cgagaatgat cgcagtgtgc aggctaagct gcaagaggaa gccagctatc 
     2881 atgcttttgg aatatttgca gagaaacaga caaaaccgca gccaaggcag aatgaggctt 
     2941 acaacagaga ggaggaaagg aaacgaaggg tctctcatga cccctttgca cagcagagag 
     3001 ctcgtgagaa tattaagagt gcaggagcaa gaggtcattc tgatcccagc acaacgagtc 
     3061 gtggaattgc agtgcaacag ctgtcatggc cagccaccca aacagtttgg aacaatggat 
     3121 tgtataatca gcatggattt ggaactacag gtacaggagt ttggtatccg ccaaatctaa 
     3181 gccaaatgta tagtacttat aaaactccag tgccagagac caacataccg ggaagcacac 
     3241 ccaccatgcc atacttctct gggccagtag cagatgacct cataaaatat actatattca 
     3301 atagttctgg tattcagatt ggaaaccaca attatatgga tgttggactg aattcacaac 
     3361 caccaaacaa tacttgcaaa gaagagtcga cttccagaca ccaagccatc tttgataaca 
     3421 ccactagtct gactgatgaa cacctgaacc ctatcaggga aaacctggga aggcagtgga 
     3481 aaaactgtgc ccgcaagctg ggcttcactg agtctcagat cgatgaaatc gaccatgact 
     3541 atgaaagaga tggactgaaa gagaaagttt accaaatgct tcagaagtgg ctgatgcggg 
     3601 aaggcaccaa aggggccaca gtgggaaagt tggcccaggc acttcaccaa tgttgcagga 
     3661 tagacctgct gaaccacttg attcgtgcca gccagagcta atctagaggg cccttcgaag 
     3721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc 
     3781 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 
     3841 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 
     3901 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 
     3961 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 
     4021 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 
     4081 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
     4141 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
     4201 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
     4261 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
     4321 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
     4381 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
     4441 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
     4501 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
     4561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     4621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     4681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     4741 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 
     4801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     4861 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 
     4921 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 
     4981 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 
     5041 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 
     5101 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 
     5161 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 
     5221 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 
     5281 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 
     5341 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 
     5401 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 
     5461 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 
     5521 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 
     5581 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 
     5641 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 
     5701 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc 
     5761 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
     5821 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 
     5881 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     5941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     6001 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 
     6061 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
     6121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     6181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     6241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     6301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     6361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     6421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 
     6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     7501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     7801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttc 
//
