ID pEF1-mRIP standard; circular DNA; ; 8089 BP. DE LMBP 4720. CC Accession number: LMBP 4720 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-MAR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(6108..6191) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(6283..6674) FT /vntifkey="33" FT /label=pMB1\ori FT CDS 4925..5719 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT CDS complement(7215..8075) FT /vntifkey="4" FT /label=ampicillin FT terminator complement(5895..5969) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 5895..5969 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin 4758..4833 FT /vntifkey="33" FT /label=SV40\ori FT promoter 4520..4801 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4087..4515 FT /vntifkey="33" FT /label=f1\ori FT terminator 3817..4041 FT /vntifkey="43" FT /label=BGH\polyA FT intron 704..1642 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT misc_marker 3771..3788 FT /vntifkey="22" FT /label=His-tag FT misc_marker 3720..3761 FT /vntifkey="22" FT /label=V5\epitope FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT CDS 1731..3701 FT /vntifkey="4" FT /label=mRIP1 FT /note="mouse receptor interacting protein 1 coding sequence" SQ Sequence 8089 BP; 1941 A; 2022 C; 2162 G; 1964 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc atgcaaccag 1740 acatgtcctt ggacaatatt aagatggcat ccagtgacct gctggagaag acagacctag 1800 acagcggagg cttcgggaag gtgtccttgt gttaccacag aagccatgga tttgtcatcc 1860 tgaaaaaagt atacacaggg cccaaccgcg ctgagtacaa tgaggttctc ttggaagagg 1920 ggaagatgat gcacagactg agacacagtc gagtggtgaa gctactgggc atcatcatag 1980 aagaagggaa ctattcgctg gtgatggagt acatggagaa gggcaacctg atgcacgtgc 2040 taaagaccca gatagatgtc ccactttcat tgaaaggaag gataatcgtg gaggccatag 2100 aaggcatgtg ctacttacat gacaaaggtg tgatacacaa ggacctgaag cctgagaata 2160 tcctcgttga tcgtgacttt cacattaaga tagccgatct tggtgtggct tcctttaaga 2220 catggagcaa actgactaag gagaaagaca acaagcagaa agaagtgagc agcaccacta 2280 agaagaacaa tggtggtacc ctttactaca tggcacccga acacctgaat gacatcaatg 2340 caaagcccac ggagaagtcg gacgtgtaca gctttggcat tgtcctttgg gcaatatttg 2400 caaaaaagga gccctatgag aatgtcatct gtactgagca gttcgtgatc tgcataaaat 2460 ctgggaacag gccaaatgta gaggaaatcc ttgagtactg tccaagggag atcatcagcc 2520 tcatggagcg gtgctggcag gcgatcccag aagacaggcc aacatttctt ggcattgaag 2580 aagaatttag gcctttttac ttaagtcatt ttgaagaata tgtagaagag gatgtggcaa 2640 gtttaaagaa agagtatcca gatcaaagcc cagtgctgca gagaatgttt tcactgcagc 2700 atgactgtgt acccttacct ccgagcaggt caaattcaga acaacctgga tcgctgcaca 2760 gttcccaggg gctccagatg ggtcctgtgg aggagtcctg gttttcttcc tccccagagt 2820 acccacagga cgagaatgat cgcagtgtgc aggctaagct gcaagaggaa gccagctatc 2880 atgcttttgg aatatttgca gagaaacaga caaaaccgca gccaaggcag aatgaggctt 2940 acaacagaga ggaggaaagg aaacgaaggg tctctcatga cccctttgca cagcagagag 3000 ctcgtgagaa tattaagagt gcaggagcaa gaggtcattc tgatcccagc acaacgagtc 3060 gtggaattgc agtgcaacag ctgtcatggc cagccaccca aacagtttgg aacaatggat 3120 tgtataatca gcatggattt ggaactacag gtacaggagt ttggtatccg ccaaatctaa 3180 gccaaatgta tagtacttat aaaactccag tgccagagac caacataccg ggaagcacac 3240 ccaccatgcc atacttctct gggccagtag cagatgacct cataaaatat actatattca 3300 atagttctgg tattcagatt ggaaaccaca attatatgga tgttggactg aattcacaac 3360 caccaaacaa tacttgcaaa gaagagtcga cttccagaca ccaagccatc tttgataaca 3420 ccactagtct gactgatgaa cacctgaacc ctatcaggga aaacctggga aggcagtgga 3480 aaaactgtgc ccgcaagctg ggcttcactg agtctcagat cgatgaaatc gaccatgact 3540 atgaaagaga tggactgaaa gagaaagttt accaaatgct tcagaagtgg ctgatgcggg 3600 aaggcaccaa aggggccaca gtgggaaagt tggcccaggc acttcaccaa tgttgcagga 3660 tagacctgct gaaccacttg attcgtgcca gccagagcta atctagaggg cccttcgaag 3720 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc 3780 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 3840 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 3900 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 3960 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 4020 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 4080 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 4140 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 4200 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 4260 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 4320 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 4380 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 4440 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 4500 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 4560 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 4620 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 4680 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 4740 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 4800 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 4860 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 4920 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 4980 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 5040 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 5100 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 5160 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 5220 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5280 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5340 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5400 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 5460 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5520 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5580 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5640 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 5700 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc 5760 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 5820 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 5880 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5940 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6000 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 6060 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6120 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 6180 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6240 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 6300 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 6360 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 6420 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6480 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6540 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6600 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6660 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6720 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6780 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6840 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 6900 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6960 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 7020 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 7080 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 7140 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 7200 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 7260 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 7320 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 7380 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 7440 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 7500 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 7560 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7620 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7680 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7740 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7800 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7860 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7920 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7980 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 8040 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttc 8089 //