LOCUS       pEF6/E-Myc-HisA         6106 bp    DNA     circular      5-JAN-2010
DEFINITION  LMBP 4743.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4743
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 5-JAN-2010
            
            
COMMENT     VNTNAME|pEF6/E-Myc-HisA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6/E-Myc-HisA" 0 6106 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "BstZI" "DraI" "HincII" "KpnI" "PvuII" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6106 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 33 0 4605 0 5851 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BstZI" "cggccg" 2 0 3 1680 0 1962 0 3351 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 292 0 853 0 2058 0 5050 0 
COMMENT            5069 0 5761 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 1 0 235 0 3171 0 3930 0 
COMMENT            0 "") #4=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1596 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 2326 0 3442 0 4126 0 0 
COMMENT            "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1627 0 1935 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "hEF1a promoter" 29 0 0 386 1529 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #8=(CFSignal (CObList) "intron hEF1a promoter" 15 0 0 582 1520 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #9=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #10=(CFSignal (CObList) "E-tag" 22 0 0 1639 1677 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1639..1677") 
COMMENT        #11=(CFSignal (CObList) "mCASP-1 fragment" 4 0 0 1678 1936 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1678..1936") 
COMMENT        #12=(CFSignal (CObList) "Myc epitope" 22 0 0 1988 2017 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1988..2017") 
COMMENT        #13=(CFSignal (CObList) "His-tag" 22 0 0 2033 2050 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2033..2050") 
COMMENT        #14=(CFSignal (CObList) "BGH polyA" 43 0 0 2079 2303 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2079..2303") 
COMMENT        #15=(CFSignal (CObList) "f1 ori" 33 0 0 2349 2777 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2349..2804") 
COMMENT        #16=(CFSignal (CObList) "SV40 early promoter" 29 0 0 2782 3063 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2782..3063") 
COMMENT        #17=(CFSignal (CObList) "SV40 ori" 33 0 0 3020 3095 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3020..3095") 
COMMENT        #18=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 3168 3233 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3168..3233") 
COMMENT        #19=(CFSignal (CObList) "blasticidin resistance" 4 0 0 3234 3632 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3234..3632") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 4178 4569 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4178..4569)") 
COMMENT        #21=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5110 5970 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5110..5970)") 
COMMENT        #22=(CFSignal (CObList) "lac promoter" 30 0 0 4003 4086 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4003..4086") 
COMMENT        #23=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3790 3864 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3768..3864)") 
COMMENT        #24=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3790 3864 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3768..3864")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #25=(CFSignal (CObList) "LMBP 4743" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "Last modified: 5-JAN-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #27=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #28=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pEF6/E-Myc-HisA" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #29=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pEF6/E-Myc-HisA'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4743." 
COMMENT                          1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/05/10 09:39" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6106 bp" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 4-JAN-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #41=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4743") 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CLinePar (CParagraph 0 (15 #26# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 5-JAN-2010"))) 
COMMENT                   "Annotations") 
COMMENT              #47=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #48=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1678 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1678  End: 1936" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of the mouse caspase-1 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1678..1936" 1))) "mCASP-1 fragment") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3234 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3234  End: 3632" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3234..3632" 1))) 
COMMENT                                 "blasticidin resistance") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5110 (3 #21# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5110  End: 5970 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5110..5970)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 582 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 582   End: 1520" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   582..1520" 1))) 
COMMENT                                 "intron hEF1a promoter"))) "Intron (1 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1639 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1639  End: 1677" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "E-tag" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1639..1677" 1))) "E-tag") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1988 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1988  End: 2017" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Myc epitope" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1988..2017" 1))) "Myc epitope") 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2033 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2033  End: 2050" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyhistidine (6 x His) tag" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2033..2050" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (3 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 386 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 386   End: 1529" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   386..1529" 1))) "hEF1a promoter") 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2782 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2782  End: 3063" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "SV40 early promoter" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2782..3063" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1546 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1546  End: 1562" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage T7 gene 10 promoter" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1546..1562" 1))) "T7 promoter") 
COMMENT                            #101=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 3168 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3168  End: 3233" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "synthetic prokaryotic EM7 promoter" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   3168..3233" 1))) 
COMMENT                                  "synthetic EM7 promoter") 
COMMENT                            #106=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 4003 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4003  End: 4086" 1) 
COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   4003..4086" 1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
COMMENT                     #110=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2349 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2349  End: 2777" 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "phage f1 origin" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2349..2804" 1))) "f1 ori") 
COMMENT                             #116=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3020 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3020  End: 3095" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "bidirectional origin of SV40" 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3020..3095" 1))) "SV40 ori") 
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COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4178 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4178  End: 4569 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "origin plasmid" 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4178..4569)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #126=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 2 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #127=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2079 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2079  End: 2303" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the bovine growth hormone" 
COMMENT                                           1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2079..2303" 1))) "BGH polyA") 
COMMENT                             #132=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3790 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3790  End: 3864 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 late region" 
COMMENT                                           1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3768..3864)" 1))) 
COMMENT                                   "SV40 polyA late") 
COMMENT                             #137=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3790 (3 #24# 0) 1 2 2 1 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3790  End: 3864" 1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 early region" 
COMMENT                                           1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #141=(CLinePar 
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COMMENT       (CStringList) 1094795585 1195852099 
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COMMENT        #416=(CSampFrag "pEF6/E-Myc-HisA" 2293 33 "Alw44I" 2326 "PvuII" 
COMMENT              (LOGPEN 0 0 0) 2.33069e-316 7.46632e-299) 
COMMENT        #417=(CSampFrag "pEF6/E-Myc-HisA" 1246 4605 "Alw44I" 5851 "Alw44I" 
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FEATURES             Location/Qualifiers
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1a\promoter
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1a\promoter
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     misc_marker     1639..1677
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             1678..1936
                     /vntifkey="4"
                     /label=mCASP-1\fragment
                     /note="fragment of the mouse caspase-1 coding sequence"
     misc_marker     1988..2017
                     /vntifkey="22"
                     /label=Myc\epitope
                     /note="Myc epitope"
     misc_marker     2033..2050
                     /vntifkey="22"
                     /label=His-tag
                     /note="polyhistidine (6 x His) tag"
     terminator      2079..2303
                     /vntifkey="43"
                     /label=BGH\polyA
                     /note="polyA signal of the bovine growth hormone"
     rep_origin      2349..2777
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     promoter        2782..3063
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /note="SV40 early promoter"
     rep_origin      3020..3095
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        3168..3233
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
                     /note="synthetic prokaryotic EM7 promoter"
     CDS             3234..3632
                     /vntifkey="4"
                     /label=blasticidin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     terminator      3790..3864
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(3790..3864)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(4178..4569)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(5110..5970)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     promoter        4003..4086
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1395 a      1541 c      1627 g      1543 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 
     1681 gccgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg 
     1741 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg 
     1801 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat 
     1861 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac 
     1921 tgctacctgg caggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 
     1981 gcccttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca 
     2041 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 
     2101 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 
     2161 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 
     2221 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 
     2281 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg 
     2341 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 
     2401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 
     2461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 
     2521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 
     2581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 
     2641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     2701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     2761 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 
     2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 
     2881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     2941 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 
     3001 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 
     3061 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 
     3121 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat 
     3181 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca 
     3241 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca 
     3301 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct 
     3361 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg 
     3421 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga 
     3481 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg 
     3541 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat 
     3601 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga 
     3661 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     3721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     3781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     3841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     3901 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     3961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     4021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     4081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     4141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     4201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     4261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     4321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     4381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     4441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     4501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     4561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     4621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     4681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     4741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 
     4801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     4861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     4921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     4981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     5041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     5101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     5161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     5221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     5281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     5341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     5401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     5461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     5521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     5581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     5641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     5701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
     5761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     5821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     5881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     5941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     6001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     6061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//
