ID pEF6/E-Myc-HisA standard; circular DNA; ; 6106 BP. DE LMBP 4743. CC Accession number: LMBP 4743 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 5-JAN-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1a\promoter FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1a\promoter FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT misc_marker 1639..1677 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS 1678..1936 FT /vntifkey="4" FT /label=mCASP-1\fragment FT /note="fragment of the mouse caspase-1 coding sequence" FT misc_marker 1988..2017 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT misc_marker 2033..2050 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 2079..2303 FT /vntifkey="43" FT /label=BGH\polyA FT /note="polyA signal of the bovine growth hormone" FT rep_origin 2349..2777 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 2782..3063 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 3020..3095 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 3168..3233 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT /note="synthetic prokaryotic EM7 promoter" FT CDS 3234..3632 FT /vntifkey="4" FT /label=blasticidin\resistance FT /note="antibiotic resistance gene; coding sequence" FT terminator 3790..3864 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(3790..3864) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(4178..4569) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5110..5970) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter 4003..4086 FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 6106 BP; 1395 A; 1541 C; 1627 G; 1543 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1620 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 1680 gccgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg 1740 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg 1800 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat 1860 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac 1920 tgctacctgg caggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 1980 gcccttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca 2040 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 2100 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 2160 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 2220 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 2280 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg 2340 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 2400 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 2460 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 2520 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 2580 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 2640 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 2700 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 2760 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 2820 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 2880 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2940 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 3000 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 3060 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 3120 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat 3180 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca 3240 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca 3300 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct 3360 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg 3420 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga 3480 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg 3540 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat 3600 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga 3660 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 3720 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3780 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 3840 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 3900 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 3960 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 4020 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 4080 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 4140 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 4200 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 4260 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 4320 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 4380 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 4440 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 4500 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 4560 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 4620 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 4680 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 4740 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 4800 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 4860 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 4920 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 4980 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5040 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5100 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 5160 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 5220 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 5280 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 5340 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 5400 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 5460 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 5520 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5580 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 5640 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5700 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 5760 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5820 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5880 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5940 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6000 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6060 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 6106 //