LOCUS       pEF1-mRIP1-ID-V5-His 6868 bp dsDNACircular
DEFINITION  LMBP 4828.
COMMENT     This file is created by Vector NTI 4.0 from InforMax, Inc., USA:
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTREPLTYPE|Plasmid|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 0 0 " all enzymes of LMBP" 1 1 1 1 0 0 1 1 0 1 10 6 
COMMENT        4000 50 0 1 0 (CHomObj 0 0 0 3 75) 
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COMMENT        (CWordArray 47 48 49 50 51 52 0 1 2 3 4 5 7 6 8 9 10 60 11 12 13 14 15 
COMMENT         16 17 18 19 20 21 22 53 23 54 24 25 26 55 27 28 29 30 31 32 33 34 35 56 
COMMENT         36 57 37 58 38 39 40 41 42 43 44 59 45 46) 
COMMENT        (CStringList "BamHI" "HindIII" "SspI" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList)) 
COMMENT       (CMolPar 0 0 0 1 6868 1 6868 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1726 0) 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 388 0 1708 0) 
COMMENT        #2=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2482 0) 
COMMENT        #3=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 4 0 1988 0 2106 0)) 
COMMENT       (CObList #4=(CFSignal (CObList) "hEF1a promoter" 29 0 0 508 1651 0) 
COMMENT        #5=(CFSignal (CObList) "intron hEF1a promoter" 15 0 0 704 1642 0) 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0) 
COMMENT        #7=(CFSignal (CObList) "V5 epitope" 22 0 0 2499 2540 0) 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 0 2550 2567 0) 
COMMENT        #9=(CFSignal (CObList) "BGH polyA" 43 0 0 2596 2820 0) 
COMMENT        #10=(CFSignal (CObList) "f1 ori" 33 0 0 2866 3294 0) 
COMMENT        #11=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3299 3580 0) 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 3537 3612 0) 
COMMENT        #13=(CFSignal (CObList) "NEO" 4 0 0 3704 4498 0) 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4674 4748 0) 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4674 4748 0) 
COMMENT        #16=(CFSignal (CObList) "pMB1 ori" 33 0 1 5062 5453 0) 
COMMENT        #17=(CFSignal (CObList) "AMP" 4 0 1 5994 6854 0) 
COMMENT        #18=(CFSignal (CObList) "mRIP1-ID" 4 0 0 1734 2480 0)) (CObList) 
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COMMENT              "DNA Plasmid pEF1-mRIP1-ID-V5-His") 
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FEATURES               Location/Qualifiers
     promoter        508..1651
                     /label=hEF1a\promoter
     intron          704..1642
                     /label=intron\hEF1a\promoter
     promoter        1668..1684
                     /label=T7\promoter
     misc_difference 2499..2540
                     /label=V5\epitope
     misc_difference 2550..2567
                     /label=His-tag
     terminator      2596..2820
                     /label=BGH\polyA
     rep_origin      2866..3294
                     /label=f1\ori
     promoter        3299..3580
                     /label=SV40\early\promoter
     rep_origin      3537..3612
                     /label=SV40\ori
     CDS             3704..4498
                     /label=NEO
     terminator      4674..4748
                     /label=SV40\polyA\early
     terminator      complement(4674..4748)
                     /label=SV40\polyA\late
     rep_origin      complement(5062..5453)
                     /label=pMB1\ori
     CDS             complement(5994..6854)
                     /label=AMP
     CDS             1734..2480
                     /label=mRIP1-ID
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc atgagtttaa 
     1741 agaaagagta tccagatcaa agcccagtgc tgcagagaat gttttcactg cagcatgact 
     1801 gtgtaccctt acctccgagc aggtcaaatt cagaacaacc tggatcgctg cacagttccc 
     1861 aggggctcca gatgggtcct gtggaggagt cctggttttc ttcctcccca gagtacccac 
     1921 aggacgagaa tgatcgcagt gtgcaggcta agctgcaaga ggaagccagc tatcatgctt 
     1981 ttggaatatt tgcagagaaa cagacaaaac cgcagccaag gcagaatgag gcttacaaca 
     2041 gagaggagga aaggaaacga agggtctctc atgacccctt tgcacagcag agagctcgtg 
     2101 agaatattaa gagtgcagga gcaagaggtc attctgatcc cagcacaacg agtcgtggaa 
     2161 ttgcagtgca acagctgtca tggccagcca cccaaacagt ttggaacaat ggattgtata 
     2221 atcagcatgg atttggaact acaggtacag gagtttggta tccgccaaat ctaagccaaa 
     2281 tgtatagtac ttataaaact ccagtgccag agaccaacat accgggaagc acacccacca 
     2341 tgccatactt ctctgggcca gtagcagatg acctcataaa atatactata ttcaatagtt 
     2401 ctggtattca gattggaaac cacaattata tggatgttgg actgaattca caaccaccaa 
     2461 acaatacttg caaagaagag tctagagggc ccttcgaagg taagcctatc cctaaccctc 
     2521 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc 
     2581 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 
     2641 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 
     2701 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 
     2761 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 
     2821 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 
     2881 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     2941 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     3001 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     3061 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     3121 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     3181 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
     3241 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 
     3301 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
     3361 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     3421 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     3481 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     3541 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
     3601 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
     3661 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 
     3721 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 
     3781 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 
     3841 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 
     3901 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 
     3961 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 
     4021 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 
     4081 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 
     4141 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 
     4201 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 
     4261 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 
     4321 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 
     4381 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 
     4441 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 
     4501 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 
     4561 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 
     4621 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 
     4681 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     4741 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     4801 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 
     4861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     4921 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     4981 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     5041 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     5101 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     5161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc 
     5221 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     5281 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     5341 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     5401 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     5461 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     5521 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     5581 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     5641 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 
     5701 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     5761 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     5821 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     5881 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     5941 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     6001 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     6061 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     6121 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     6181 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     6241 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     6301 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     6361 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     6421 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     6481 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     6541 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     6601 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     6661 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     6721 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     6781 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     6841 tgttgaatac tcatactctt cctttttc 
//
