LOCUS       pEF1-mRIP1-DD-V5-His 6433 bp dsDNACircular
DEFINITION  LMBP 4829.
COMMENT     This file is created by Vector NTI 4.0 from InforMax, Inc., USA:
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTREPLTYPE|Plasmid|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 0 0 " all enzymes of LMBP" 1 1 1 1 0 0 1 1 0 1 10 6 
COMMENT        4000 50 0 1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray 47 48 49 50 51 52 0 1 2 3 4 5 7 6 8 9 10 60 11 12 13 14 15 
COMMENT         16 17 18 19 20 21 22 53 23 54 24 25 26 55 27 28 29 30 31 32 33 34 35 56 
COMMENT         36 57 37 58 38 39 40 41 42 43 44 59 45 46) 
COMMENT        (CStringList "BamHI" "HindIII" "KpnI" "SspI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList)) 
COMMENT       (CMolPar 0 0 0 1 6433 1 6433 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1726 0) 
COMMENT        #1=(CRSite (CStringList) "SspI" "aatatt" 4 0 1 4 0) 
COMMENT        #2=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2047 0) 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 388 0 1708 0) 
COMMENT        #4=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1718 0)) 
COMMENT       (CObList #5=(CFSignal (CObList) "hEF1a promoter" 29 0 0 508 1651 0) 
COMMENT        #6=(CFSignal (CObList) "intron hEF1a promoter" 15 0 0 704 1642 0) 
COMMENT        #7=(CFSignal (CObList) "V5 epitope" 22 0 0 2064 2105 0) 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 0 2115 2132 0) 
COMMENT        #9=(CFSignal (CObList) "BGH polyA" 43 0 0 2161 2385 0) 
COMMENT        #10=(CFSignal (CObList) "f1 ori" 33 0 0 2431 2859 0) 
COMMENT        #11=(CFSignal (CObList) "SV40 early promoter" 29 0 0 2864 3145 0) 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 3102 3177 0) 
COMMENT        #13=(CFSignal (CObList) "NEO" 4 0 0 3269 4063 0) 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4239 4313 0) 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4239 4313 0) 
COMMENT        #16=(CFSignal (CObList) "pMB1 ori" 33 0 1 4627 5018 0) 
COMMENT        #17=(CFSignal (CObList) "AMP" 4 0 1 5559 6419 0) 
COMMENT        #18=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0) 
COMMENT        #19=(CFSignal (CObList) "mRIP1-DD" 4 0 0 1734 2045 0)) (CObList) 
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COMMENT              "DNA Plasmid pEF1-mRIP1-DD-V5-His") 
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FEATURES               Location/Qualifiers
     promoter        508..1651
                     /label=hEF1a\promoter
     intron          704..1642
                     /label=intron\hEF1a\promoter
     misc_difference 2064..2105
                     /label=V5\epitope
     misc_difference 2115..2132
                     /label=His-tag
     terminator      2161..2385
                     /label=BGH\polyA
     rep_origin      2431..2859
                     /label=f1\ori
     promoter        2864..3145
                     /label=SV40\early\promoter
     rep_origin      3102..3177
                     /label=SV40\ori
     CDS             3269..4063
                     /label=NEO
     terminator      4239..4313
                     /label=SV40\polyA\early
     terminator      complement(4239..4313)
                     /label=SV40\polyA\late
     rep_origin      complement(4627..5018)
                     /label=pMB1\ori
     CDS             complement(5559..6419)
                     /label=AMP
     promoter        1668..1684
                     /label=T7\promoter
     CDS             1734..2045
                     /label=mRIP1-DD
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc atgtcgactt 
     1741 ccagacacca agccatcttt gataacacca ctagtctgac tgatgaacac ctgaacccta 
     1801 tcagggaaaa cctgggaagg cagtggaaaa actgtgcccg caagctgggc ttcactgagt 
     1861 ctcagatcga tgaaatcgac catgactatg aaagagatgg actgaaagag aaagtttacc 
     1921 aaatgcttca gaagtggctg atgcgggaag gcaccaaagg ggccacagtg ggaaagttgg 
     1981 cccaggcact tcaccaatgt tgcaggatag acctgctgaa ccacttgatt cgtgccagcc 
     2041 agagctctag agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt 
     2101 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga 
     2161 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 
     2221 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 
     2281 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 
     2341 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 
     2401 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 
     2461 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     2521 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     2581 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     2641 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     2701 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     2761 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     2821 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 
     2881 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     2941 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     3001 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 
     3061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     3121 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 
     3181 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 
     3241 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     3301 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     3361 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     3421 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     3481 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     3541 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 
     3601 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     3661 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     3721 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     3781 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     3841 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     3901 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     3961 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     4021 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 
     4081 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 
     4141 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 
     4201 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 
     4261 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     4321 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 
     4381 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     4441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     4501 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     4561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     4621 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     4681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     4741 caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     4801 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     4861 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     4921 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     4981 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     5041 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     5101 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     5161 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     5221 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     5281 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     5341 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     5401 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     5461 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     5521 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     5581 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     5641 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     5701 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     5761 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     5821 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     5881 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     5941 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     6001 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     6061 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     6121 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     6181 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     6241 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     6301 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     6361 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     6421 ctcttccttt ttc 
//
