LOCUS       pEF6-His-PKR-ND1-228        6755 bp    DNA     circular     29-SEP-2010
DEFINITION  LMBP 4847.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 3964
            Last modified: 20-NOV-2009
            
            
            
            
COMMENT     VNTDATE|566568002|
COMMENT     VNTNAME|pEF6-His-PKR-ND1-228|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-His-PKR-ND1-228" 0 6755 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "BssHII" "EcoRI" "SacII" "XbaI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6755 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1627 0 2073 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 606 0 953 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1666 0 2623 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 4 1317 0 1660 0 1681 0 2569 
COMMENT            0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #6=(CFSignal (CObList) "His-tag" 22 0 0 2682 2699 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1725..1742") 
COMMENT        #7=(CFSignal (CObList) "BGH polyA" 43 0 0 2728 2952 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1771..1995") 
COMMENT        #8=(CFSignal (CObList) "f1 ori" 33 0 0 2998 3426 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2041..2469") 
COMMENT        #9=(CFSignal (CObList) "blasticidin resistance" 4 0 0 3883 4281 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2926..3324") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4439 4513 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3482..3556") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4439 4513 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3482..3556)") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 4827 5218 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3870..4261)") 
COMMENT        #13=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3431 3712 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2474..2755") 
COMMENT        #14=(CFSignal (CObList) "SV40 ori" 33 0 0 3669 3744 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2712..2787") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5759 6619 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4802..5662)") 
COMMENT        #16=(CFSignal (CObList) "hEF1a promoter" 29 0 0 386 1529 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #17=(CFSignal (CObList) "Myc epitope" 22 0 0 2637 2666 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1680..1709") 
COMMENT        #18=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 3817 3882 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2860..2925") 
COMMENT        #19=(CFSignal (CObList) "intron hEF1a promoter" 15 0 0 582 1520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #20=(CFSignal (CObList) "lac promoter" 30 0 1 4652 4735 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3695..3778)") 
COMMENT        #21=(CFSignal (CObList) "hPKR-ND 1-228" 4 0 0 1740 2534 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "Coll. N\260.: 3964" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 20-NOV-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pEF6-His-PKR-ND1-228" 2) 5 "" 0 4) 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pEF6-His-PKR-ND1-228'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DMB 3964  (Invitrogen vector)." 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/29/10 12:00" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 09/29/10 12:00" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6755 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-NOV-2009" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Coll. N\260.: 3964") 
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COMMENT                           (CObjectList 
COMMENT                            #43=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 20-NOV-2009"))) 
COMMENT                   "Annotations") 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
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COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1740  End: 2534" 1))) 
COMMENT                                 "hPKR-ND 1-228") 
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COMMENT                                  (CParagraph 3883 (3 #9# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3883  End: 4281" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2926..3324" 1))) 
COMMENT                                 "blasticidin resistance") 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5759 (3 #15# 0) 1 2 2 0 327) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5759  End: 6619 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4802..5662)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   582..1520" 1))) 
COMMENT                                 "intron hEF1a promoter"))) "Intron (1 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1680..1709" 1))) "Myc epitope") 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2682  End: 2699" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1725..1742" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 386   End: 1529" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   386..1529" 1))) "hEF1a promoter") 
COMMENT                            #75=(CFolderPar 
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COMMENT                                  (CParagraph 3431 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
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COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2474..2755" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1546..1562" 1))) "T7 promoter") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3817 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2860..2925" 1))) 
COMMENT                                 "synthetic EM7 promoter") 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4652 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                   #90=(CLinePar 
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COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3695..3778)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                   #96=(CLinePar 
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COMMENT                                          "   complement(3870..4261)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                     #109=(CLinePar 
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COMMENT                                           "   1771..1995" 1))) "BGH polyA") 
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COMMENT                                     #113=(CLinePar 
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COMMENT                                   "SV40 polyA early") 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3482..3556)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                              (CObjectList)) "BssHII: 0 sites") 1 5 "GCGCGC" 
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COMMENT                                      #123=(CLinePar 
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COMMENT                                            " N2: 2073 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                                            " N1: 606 " 1) 
COMMENT                                      #127=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 953 " 1))))) "SacII: 2 sites") 
COMMENT                            4 2 "CCGCGG" "GGCGCC") 
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COMMENT                            1 5 "TCTAGA" "AGATCT") 
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COMMENT                                            " N1: 1317 " 1) 
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COMMENT                                            " N2: 1660 " 1) 
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COMMENT                                            " N3: 1681 " 1) 
COMMENT                                      #137=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
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FEATURES             Location/Qualifiers
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     2682..2699
                     /vntifkey="22"
                     /label=His-tag
     terminator      2728..2952
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      2998..3426
                     /vntifkey="33"
                     /label=f1\ori
     CDS             3883..4281
                     /vntifkey="4"
                     /label=blasticidin\resistance
     terminator      4439..4513
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4439..4513)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4827..5218)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3431..3712
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3669..3744
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(5759..6619)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1a\promoter
     misc_marker     2637..2666
                     /vntifkey="22"
                     /label=Myc\epitope
     promoter        3817..3882
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1a\promoter
     promoter        complement(4652..4735)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             1740..2534
                     /vntifkey="4"
                     /label=hPKR-ND\1-228
BASE COUNT     1608 a      1668 c      1756 g      1715 t         8 others
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga tccctcgacc 
     1681 tcgagatcca ttgtgctggn nnnaaataat tttgtttaac tttaagaagg agatatacca 
     1741 tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata 
     1801 tgatggctgg tgatctttca gcaggtttct tcatggagga acttaataca taccgtcaga 
     1861 agcagggagt agtacttaaa tatcaagaac tgcctaattc aggacctcca catgatagga 
     1921 ggtttacatt tcaagttata atagatggaa gagaatttcc agaaggtgaa ggtagatcaa 
     1981 agaaggaagc aaaaaatgcc gcagccaaat tagctgttga gatacttaat aaggaaaaga 
     2041 aggcagttag tcctttatta ttgacaacaa cgaattcttc agaaggatta tccatgggga 
     2101 attacatagg ccttatcaat agaattgccc agaagaaaag actaactgta aattatgaac 
     2161 agtgtgcatc gggggtgcat gggccagaag gatttcatta taaatgcaaa atgggacaga 
     2221 aagaatatag tattggtaca ggttctacta aacaggaagc aaaacaattg gccgctaaac 
     2281 ttgcatatct tcagatatta tcagaagaaa cctcagtgaa atctgactac ctgtcctctg 
     2341 gttcttttgc tactacgtgt gagtcccaaa gcaactcttt agtgaccagc acactcgctt 
     2401 ctgaatcatc atctgaaggt gacttctcag cagatacatc agagataaat tctaacagtg 
     2461 acagtttaaa cagttcttcg ttgcttatga atggtctcag aaataatcaa aggaaggcaa 
     2521 aaagatcttt gtgagcggcc gcaaaaaaan nnnccagcac aatggatctc gagggatctt 
     2581 ccatacctac cagttctgcg cctgcaggtc gcggccgcga ctctagaggg cccttcgaac 
     2641 aaaaactcat ctcagaagag gatctgaata tgcataccgg tcatcatcac catcaccatt 
     2701 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 
     2761 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
     2821 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 
     2881 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 
     2941 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 
     3001 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     3061 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     3121 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     3181 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     3241 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     3301 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     3361 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     3421 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 
     3481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
     3541 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
     3601 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     3661 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 
     3721 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 
     3781 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag 
     3841 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct 
     3901 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct 
     3961 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc 
     4021 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct 
     4081 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc 
     4141 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc 
     4201 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct 
     4261 ggttatgtgt gggagggcta agcacttcgt ggccgaggag caggactgac acgtgctacg 
     4321 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 
     4381 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 
     4441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     4501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     4561 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 
     4621 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 
     4681 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 
     4741 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 
     4801 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     4861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     4921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagg aaccgtaaaa 
     4981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     5041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     5101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     5161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     5221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     5281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     5341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     5401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     5461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     5521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     5581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     5641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     5701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     5761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     5821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     5881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     5941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     6001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     6061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     6121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     6181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     6241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     6301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     6361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     6421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     6481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     6541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     6601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     6661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     6721 cgcgcacatt tccccgaaaa gtgccacctg acgtc 
//
