LOCUS       pEF6-Etag-mcPLA2        8069 bp    DNA     circular      7-APR-2008
DEFINITION  LMBP 4930.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4930
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 7-APR-2008
            
            
COMMENT     VNTNAME|pEF6-Etag-mcPLA2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-Etag-mcPLA2" 0 8069 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BspTI" "EcoRI" "HincII" "NcoI" "NotI" "PvuII" 
COMMENT         "SspI" "XbaI" "PvuI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "mcPLA2 (1)" 
COMMENT             "ATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTCCCATAAGTTTACTGTTGTGGTTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGACACTCCAGATCCTTATGTGGAACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCAATAATGATATAAACCCCGTGTGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATCACACTGATGGATGCCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCTTCAATGAAAGTGGGAGAGAAGAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAATGTCTCTGGAAGTTTGTTCATGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTTCAGACAGCAGAGGAAAGAGAACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGGCTTTATTCCACACGTGATGTGCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGGGATTCTCTGGTGTGATGAAGGCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCTTTCTGGATCCACATGGTACATGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAGATTAATGAAGAGCTAATGAAAAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAAGATACGTTGAGTCTTTATGGAAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGATGCTAATAGGAGAAACACTAATTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAATGCCGCCCGGTGTCCTTTGCCTCTCTTCACGTGTCTCCACG" 
COMMENT             "Fragment of pEF6-Etag-mcPLA2; Length: 1000" 0 (CStringList) 0) 
COMMENT         #1=(COligo "mcPLA2 (2)" 
COMMENT             "TCAAACCTGATGTGTCAGAGCTGATGTTTGCCGATTGGGTGGAATTTAGTCCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCTATTTGGAAGCAAGTTTTTTATGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATGGGTGTCTGGGGCAGTGCCTTTTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAGGCTCTACAATGGAAGAGGAATTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAGTGATGATGAGGCTCAAGGACCCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAACCAAGCAAGTTGGGTCCATCGGATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAGGACGTGCCGGAAAGGTGCATAACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCTGAGAGACTTCAGCTCTCAGGATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTTGAACGAATATATGAACCACTGGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTAACCTGCCGTATCCCTTGATTCTGCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGCAAGGCCGAGTGACACCAGTCCCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAGCTTCCCTTTCCAAAGATCGATCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAACCTAAGAATCCTGATGTGGAGAAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCCCCAGGTGTTCTAAGGGAAACCAAAGAAGAGAAAGAAATCGCTGACTT" 
COMMENT             "Fragment of pEF6-Etag-mcPLA2; Length: 1000" 0 (CStringList) 0) 
COMMENT         #2=(COligo "mcPLA2 (3)" 
COMMENT             "TGACATTTTTGATGACCCCGAATCGCCATTTTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTGCATGATCTGATGTACTTCAACACACTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATACAGAAGACAGAACCCATCTCGTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAGGAGTTTCTAAGTAAACCCACTGTGTAA" 
COMMENT             "Fragment of pEF6-Etag-mcPLA2; Length: 247" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 8069 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 1604 0 1924 0 2372 0 0 
COMMENT            "") #4=(CRSite (CStringList) "BspTI" "cttaag" 2 0 1 731 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1627 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 1 0 235 0 2391 0 5134 0 
COMMENT            5893 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 1635 0 2289 0 4976 0 5196 
COMMENT            0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1680 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 4289 0 5405 0 6089 0 0 
COMMENT            "") 
COMMENT        #10=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 2956 0 7951 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3937 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 5433 0 7517 0 0 "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #14=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 582 1520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #15=(CFSignal (CObList) "hEF1 promoter" 29 0 0 386 1529 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #16=(CFSignal (CObList) "E-tag" 22 0 0 1639 1677 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1639..1677") 
COMMENT        #17=(CFSignal (CObList) "Myc epitope" 22 0 0 3951 3980 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3951..3980") 
COMMENT        #18=(CFSignal (CObList) "His-tag" 22 0 0 3996 4013 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3996..4013") 
COMMENT        #19=(CFSignal (CObList) "bovine growth hormone polyA" 43 0 0 4042 4266 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4042..4266") 
COMMENT        #20=(CFSignal (CObList) "f1 ori" 33 0 0 4312 4740 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4312..4740") 
COMMENT        #21=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4745 5026 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4745..5026") 
COMMENT        #22=(CFSignal (CObList) "SV40 ori" 33 0 0 4983 5058 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4983..5058") 
COMMENT        #23=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 5131 5196 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5131..5196") 
COMMENT        #24=(CFSignal (CObList) "blasticidin" 4 0 0 5197 5595 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5197..5595") 
COMMENT        #25=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5753 5827 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5753..5827") 
COMMENT        #26=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5753 5827 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5753..5827)") 
COMMENT        #27=(CFSignal (CObList) "pMB1 ori" 33 0 1 6141 6532 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6141..6532)") 
COMMENT        #28=(CFSignal (CObList) "ampicillin" 4 0 1 7073 7933 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7073..7933)") 
COMMENT        #29=(CFSignal (CObList) "mcPLA2" 4 0 0 1687 3933 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1687..3933") 
COMMENT        #30=(CFSignal (CObList) "lac promoter" 30 0 1 5966 6049 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5966..6049)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #31=(CFSignal (CObList) "LMBP 4930" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #32=(CFSignal (CObList) "Last modified: 7-APR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #33=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #34=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pEF6-Etag-mcPLA2" 
COMMENT                    2) 5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA Plasmid 'pEF6-Etag-mcPLA2'" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4930." 
COMMENT                          1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/07/08 11:00" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8069 bp" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 9-NOV-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #47=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                     #49=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 4930") 
COMMENT                     #51=(CFolderPar 
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COMMENT                                 "" 1))) "Last modified: 7-APR-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #53=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1687  End: 3933" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1687..3933" 1))) "mcPLA2") 
COMMENT                            #59=(CFolderPar 
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COMMENT                                        "Start: 5197  End: 5595" 1) 
COMMENT                                   #61=(CLinePar 
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COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5197..5595" 1))) "blasticidin") 
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COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7073  End: 7933 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #65=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7073..7933)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (3 total)") 
COMMENT                     #67=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 582   End: 1520" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   582..1520" 1))) 
COMMENT                                 "intron hEF1 promoter"))) "Intron (1 total)") 
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COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1639..1677" 1))) "E-tag") 
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COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3951  End: 3980" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3951..3980" 1))) "Myc epitope") 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3996 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
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COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3996..4013" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (3 total)") 
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COMMENT                                        "Start: 386   End: 1529" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
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COMMENT                                        "   386..1529" 1))) "hEF1 promoter") 
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COMMENT                                   #93=(CLinePar 
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COMMENT                                        "   4745..5026" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #98=(CLinePar 
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COMMENT                                        "   1546..1562" 1))) "T7 promoter") 
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COMMENT                                   #102=(CLinePar 
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COMMENT                                         "   5131..5196" 1))) 
COMMENT                                 "synthetic EM7 promoter") 
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COMMENT                                          "   complement(5966..6049)" 1))) 
COMMENT                                  "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                           "   4312..4740" 1))) "f1 ori") 
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COMMENT                                           "   complement(6141..6532)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (3 total)") 
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FEATURES             Location/Qualifiers
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1\promoter
     misc_marker     1639..1677
                     /vntifkey="22"
                     /label=E-tag
     misc_marker     3951..3980
                     /vntifkey="22"
                     /label=Myc\epitope
     misc_marker     3996..4013
                     /vntifkey="22"
                     /label=His-tag
     terminator      4042..4266
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
     rep_origin      4312..4740
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4745..5026
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4983..5058
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        5131..5196
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     CDS             5197..5595
                     /vntifkey="4"
                     /label=blasticidin
     terminator      5753..5827
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5753..5827)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(6141..6532)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7073..7933)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             1687..3933
                     /vntifkey="4"
                     /label=mcPLA2
     promoter        complement(5966..6049)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1959 a      1960 c      2075 g      2075 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 
     1681 gccgctatgt ctttcataga tccttatcag cacattatag tggaacacca gtactcccat 
     1741 aagtttactg ttgtggttct acgtgccacc aaagtaacca aggggacctt tggcgatatg 
     1801 ctggacactc cagatcctta tgtggaactt ttcatctcta caacccctga cagcaggaag 
     1861 cgaacgagac acttcaataa tgatataaac cccgtgtgga atgagacctt tgagttcatt 
     1921 ttggatccta atcaggaaaa tgttttggag atcacactga tggatgccaa ctacgtcatg 
     1981 gatgaaaccc taggcacagc tacattccct gtatcttcaa tgaaagtggg agagaagaaa 
     2041 gaagtccctt ttattttcaa ccaagtcact gaaatgattc tggaaatgtc tctggaagtt 
     2101 tgttcatgcc cagacctacg gttcagcatg gcactgtgtg atcaggagaa aactttcaga 
     2161 cagcagagga aagagaacat aaaagagaac atgaagaaac ttttgggtcc aaaaaagagt 
     2221 gaggggcttt attccacacg tgatgtgccg gtggtggcca ttttgggttc aggtgggggt 
     2281 ttccgggcca tggtgggatt ctctggtgtg atgaaggcac tgtatgagtc ggggattttg 
     2341 gactgtgcta catacattgc tggtctttct ggatccacat ggtacatgtc aaccttgtac 
     2401 tctcaccccg attttccaga gaaaggtccc gaggagatta atgaagagct aatgaaaaat 
     2461 gtcagccaca accctctctt acttcttaca ccacagaaag ttaaaagata cgttgagtct 
     2521 ttatggaaga agaaaagttc tggccagcct gtcaccttta ctgacatctt tgggatgcta 
     2581 ataggagaaa cactaattca aaataggatg agcatgaccc tgagtagttt gaaggaaaag 
     2641 gtcaatgccg cccggtgtcc tttgcctctc ttcacgtgtc tccacgtcaa acctgatgtg 
     2701 tcagagctga tgtttgccga ttgggtggaa tttagtccat atgagattgg catggcaaaa 
     2761 tatggtacct ttatggctcc tgacctattt ggaagcaagt tttttatggg aacagttgta 
     2821 aaaaaatatg aagaaaaccc cttgcatttc ttgatgggtg tctggggcag tgccttttct 
     2881 atactgttca acagagtttt gggagtttct ggctcacaga ataaaggctc tacaatggaa 
     2941 gaggaattag aaaatattac agcaaagcac atcgtgagta atgacagctc cgacagtgat 
     3001 gatgaggctc aaggacccaa aggcaccgag aatgaagaag ctgaaaaaga gtaccaaagc 
     3061 gacaaccaag caagttgggt ccatcggatg ctaatggcct tggtgagcga ctcggcttta 
     3121 ttcaataccc gagaaggacg tgccggaaag gtgcataact tcatgctggg cttgaatctc 
     3181 aacacatcat atccactgtc tcccctgaga gacttcagct ctcaggattc cttcgatgac 
     3241 gagctcgacg cagcggtagc agatccagat gaatttgaac gaatatatga accactggat 
     3301 gtcaaaagta agaagattca tgtggtagat agtgggctca catttaacct gccgtatccc 
     3361 ttgattctgc gacctcagag aggtgtggat cttatcatct cctttgactt ttctgcaagg 
     3421 ccgagtgaca ccagtccccc tttcaaggaa cttctgcttg cagagaagtg ggcgaaaatg 
     3481 aacaagcttc cctttccaaa gatcgatcct tatgtgtttg atcgggaagg attaaaggaa 
     3541 tgctatgttt ttaaacctaa gaatcctgat gtggagaagg attgcccaac cattatccac 
     3601 tttgttctgg ccaacatcaa cttcagaaag tacaaggccc caggtgttct aagggaaacc 
     3661 aaagaagaga aagaaatcgc tgactttgac atttttgatg accccgaatc gccattttca 
     3721 accttcaact ttcagtatcc caatcaagca ttcaaaaggc tgcatgatct gatgtacttc 
     3781 aacacactga acaacattga tgtgataaag gatgccattg ttgagagcat tgaatacaga 
     3841 agacagaacc catctcgttg ctctgtttcc ctcagtaatg ttgaagcaag aaaattcttc 
     3901 aataaggagt ttctaagtaa acccactgtg taaggtctag agggcccttc gaacaaaaac 
     3961 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt 
     4021 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     4081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     4141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     4201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     4261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 
     4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     4381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     4501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     4621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     4681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     4741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     4801 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     4861 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     4921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     4981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 
     5041 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 
     5101 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 
     5161 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa 
     5221 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac 
     5281 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta 
     5341 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg 
     5401 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc 
     5461 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg 
     5521 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat 
     5581 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt 
     5641 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 
     5701 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 
     5761 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     5821 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     5881 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 
     5941 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     6001 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     6061 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     6121 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     6181 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     6241 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg 
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     6481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     6721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     6841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     6901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     6961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     7021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     7081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     7141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     7201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     7261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     7321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     7381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     7441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     7501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     7561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     7621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     7681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     7741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     7801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     7861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     7921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     7981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     8041 catttccccg aaaagtgcca cctgacgtc 
//
