ID pEF6-Etag-mcPLA2 standard; circular DNA; ; 8069 BP. DE LMBP 4930. CC Accession number: LMBP 4930 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 7-APR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1\promoter FT misc_marker 1639..1677 FT /vntifkey="22" FT /label=E-tag FT misc_marker 3951..3980 FT /vntifkey="22" FT /label=Myc\epitope FT misc_marker 3996..4013 FT /vntifkey="22" FT /label=His-tag FT terminator 4042..4266 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT rep_origin 4312..4740 FT /vntifkey="33" FT /label=f1\ori FT promoter 4745..5026 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4983..5058 FT /vntifkey="33" FT /label=SV40\ori FT promoter 5131..5196 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT CDS 5197..5595 FT /vntifkey="4" FT /label=blasticidin FT terminator 5753..5827 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(5753..5827) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(6141..6532) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7073..7933) FT /vntifkey="4" FT /label=ampicillin FT CDS 1687..3933 FT /vntifkey="4" FT /label=mcPLA2 FT promoter complement(5966..6049) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 8069 BP; 1959 A; 1960 C; 2075 G; 2075 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1620 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 1680 gccgctatgt ctttcataga tccttatcag cacattatag tggaacacca gtactcccat 1740 aagtttactg ttgtggttct acgtgccacc aaagtaacca aggggacctt tggcgatatg 1800 ctggacactc cagatcctta tgtggaactt ttcatctcta caacccctga cagcaggaag 1860 cgaacgagac acttcaataa tgatataaac cccgtgtgga atgagacctt tgagttcatt 1920 ttggatccta atcaggaaaa tgttttggag atcacactga tggatgccaa ctacgtcatg 1980 gatgaaaccc taggcacagc tacattccct gtatcttcaa tgaaagtggg agagaagaaa 2040 gaagtccctt ttattttcaa ccaagtcact gaaatgattc tggaaatgtc tctggaagtt 2100 tgttcatgcc cagacctacg gttcagcatg gcactgtgtg atcaggagaa aactttcaga 2160 cagcagagga aagagaacat aaaagagaac atgaagaaac ttttgggtcc aaaaaagagt 2220 gaggggcttt attccacacg tgatgtgccg gtggtggcca ttttgggttc aggtgggggt 2280 ttccgggcca tggtgggatt ctctggtgtg atgaaggcac tgtatgagtc ggggattttg 2340 gactgtgcta catacattgc tggtctttct ggatccacat ggtacatgtc aaccttgtac 2400 tctcaccccg attttccaga gaaaggtccc gaggagatta atgaagagct aatgaaaaat 2460 gtcagccaca accctctctt acttcttaca ccacagaaag ttaaaagata cgttgagtct 2520 ttatggaaga agaaaagttc tggccagcct gtcaccttta ctgacatctt tgggatgcta 2580 ataggagaaa cactaattca aaataggatg agcatgaccc tgagtagttt gaaggaaaag 2640 gtcaatgccg cccggtgtcc tttgcctctc ttcacgtgtc tccacgtcaa acctgatgtg 2700 tcagagctga tgtttgccga ttgggtggaa tttagtccat atgagattgg catggcaaaa 2760 tatggtacct ttatggctcc tgacctattt ggaagcaagt tttttatggg aacagttgta 2820 aaaaaatatg aagaaaaccc cttgcatttc ttgatgggtg tctggggcag tgccttttct 2880 atactgttca acagagtttt gggagtttct ggctcacaga ataaaggctc tacaatggaa 2940 gaggaattag aaaatattac agcaaagcac atcgtgagta atgacagctc cgacagtgat 3000 gatgaggctc aaggacccaa aggcaccgag aatgaagaag ctgaaaaaga gtaccaaagc 3060 gacaaccaag caagttgggt ccatcggatg ctaatggcct tggtgagcga ctcggcttta 3120 ttcaataccc gagaaggacg tgccggaaag gtgcataact tcatgctggg cttgaatctc 3180 aacacatcat atccactgtc tcccctgaga gacttcagct ctcaggattc cttcgatgac 3240 gagctcgacg cagcggtagc agatccagat gaatttgaac gaatatatga accactggat 3300 gtcaaaagta agaagattca tgtggtagat agtgggctca catttaacct gccgtatccc 3360 ttgattctgc gacctcagag aggtgtggat cttatcatct cctttgactt ttctgcaagg 3420 ccgagtgaca ccagtccccc tttcaaggaa cttctgcttg cagagaagtg ggcgaaaatg 3480 aacaagcttc cctttccaaa gatcgatcct tatgtgtttg atcgggaagg attaaaggaa 3540 tgctatgttt ttaaacctaa gaatcctgat gtggagaagg attgcccaac cattatccac 3600 tttgttctgg ccaacatcaa cttcagaaag tacaaggccc caggtgttct aagggaaacc 3660 aaagaagaga aagaaatcgc tgactttgac atttttgatg accccgaatc gccattttca 3720 accttcaact ttcagtatcc caatcaagca ttcaaaaggc tgcatgatct gatgtacttc 3780 aacacactga acaacattga tgtgataaag gatgccattg ttgagagcat tgaatacaga 3840 agacagaacc catctcgttg ctctgtttcc ctcagtaatg ttgaagcaag aaaattcttc 3900 aataaggagt ttctaagtaa acccactgtg taaggtctag agggcccttc gaacaaaaac 3960 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt 4020 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 4080 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 4140 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 4200 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 4260 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 4320 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 4380 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 4440 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 4500 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 4560 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4620 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4680 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4740 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 4800 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 4860 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 4920 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 4980 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 5040 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 5100 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 5160 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa 5220 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac 5280 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta 5340 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg 5400 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc 5460 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg 5520 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat 5580 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt 5640 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 5700 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 5760 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 5820 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 5880 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 5940 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6000 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6060 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6120 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6180 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6240 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg 6300 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6360 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 6420 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6480 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6540 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6600 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 6660 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 6720 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 6780 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 6840 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 6900 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 6960 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7020 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7080 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7140 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7200 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7260 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7320 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7380 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7440 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7500 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7560 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7620 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7680 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7740 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7800 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7860 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 7920 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 7980 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8040 catttccccg aaaagtgcca cctgacgtc 8069 //