LOCUS       pDEST-MycMAS 6176 bp dsDNA   Circular
DEFINITION  LMBP 5025.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(526..928)
                     /label=pMB1\ori
     CDS             complement(1469..2329)
                     /label=AMP
     CDS             4585..5244
                     /label=CAM
     CDS             5586..5891
                     /label=ccdB
     rep_origin      complement(2460..2915)
                     /label=f1\ori
     promoter        4008..4024
                     /label=SP6\promoter
     attenuator      5932..6056
                     /label=attR2
     attenuator      complement(4352..4476)
                     /label=attR1
     promoter        2989..4001
                     /label=sCMV\promoter
     promoter        complement(1..17)
                     /label=T7\promoter
     terminator      complement(82..178)
                     /label=SV40\polyA\early
     terminator      82..178
                     /label=SV40\polyA\late
     promoter        complement(271..287)
                     /label=T3\promoter
     misc_difference 4295..4324
                     /label=Myc\epitope
     misc_difference 4232..4261
                     /label=Myc\epitope
     misc_difference 4193..4222
                     /label=Myc\epitope
     misc_difference 4154..4183
                     /label=Myc\epitope
     misc_difference 4115..4144
                     /label=Myc\epitope
     misc_difference 4076..4105
                     /label=Myc\epitope
     misc_difference 6073..6168
                     /label=MAS
ORIGIN
        1 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa 
       61 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 
      121 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 
      181 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg 
      241 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 
      301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
      361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
      421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
      481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
      541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
      601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
      661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
      721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
      781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
      841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
      901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
      961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     1021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     1081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     1141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     1201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     1261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     1321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     1381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     1441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     1501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     1561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     1621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     1681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     1741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     1801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     1861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     1921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     1981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     2041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     2101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     2161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     2221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     2281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     2341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     2401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta 
     2461 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 
     2521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 
     2581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 
     2641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 
     2701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 
     2761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 
     2821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 
     2881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa 
     2941 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa 
     3001 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa 
     3061 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa 
     3121 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa 
     3181 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca 
     3241 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag 
     3301 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc 
     3361 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt 
     3421 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc 
     3481 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat 
     3541 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg 
     3601 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga 
     3661 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat 
     3721 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg 
     3781 gaaggacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg 
     3841 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg 
     3901 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc 
     3961 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac 
     4021 tatagaatac aagctacttg ttctttttgc aggatcccat cgatttaaag ctatggagca 
     4081 aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt ctgaagagga 
     4141 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa 
     4201 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt 
     4261 gaatgaaatg gagagcttgg gcgacctcac catggagcaa aagctcattt ctgaagagga 
     4321 cttgaattta gagatctcga attcaaggat cacaagtttg tacaaaaaag ctgaacgaga 
     4381 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac 
     4441 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc 
     4501 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga 
     4561 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat 
     4621 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc 
     4681 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag 
     4741 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa 
     4801 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 
     4861 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 
     4921 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 
     4981 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 
     5041 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 
     5101 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 
     5161 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 
     5221 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag 
     5281 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga 
     5341 agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca 
     5401 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat 
     5461 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat 
     5521 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg 
     5581 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg 
     5641 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg 
     5701 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg 
     5761 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag 
     5821 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct 
     5881 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac 
     5941 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 
     6001 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatc 
     6061 ctctcgatcg aggggaacca tacgacgtta attcttgcaa tgttagctat tggcgtgttc 
     6121 tctttagggg cgtttatcaa aattattcaa ttaagaaaaa ataattaatc tagaac 
//
