LOCUS       pUC19                   2686 bp    DNA     circular      3-MAR-2011
DEFINITION  LMBP 5026.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5026
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 3-MAR-2011
            
            
            
            
COMMENT     VNTNAME|pUC19|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pUC19" 0 2686 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "BamHI" "BglI" "PstI" "PvuII" "RsaI" "XmnI" "HindII" 
COMMENT         "EcoRI" "HindIII" "KpnI" "SacI" "SalI" "SmaI" "SphI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 239 469 1 2686 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 418 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 252 0 1820 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 440 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 309 0 631 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "RsaI" "gtac" 3 0 3 170 0 411 0 2180 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 2299 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindII" "gtyrac" 4 0 1 432 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 397 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 448 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 413 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 407 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 430 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 415 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 446 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 424 0 0 "")) 
COMMENT       (CObList 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(470..507)") 
COMMENT        #16=(CFSignal (CObList) "lac promoter" 30 0 1 508 591 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(508..591)") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 683 1085 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(683..1085)") 
COMMENT        #18=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1626 2486 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1626..2486)") 
COMMENT        #19=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 1 236 469 0 
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COMMENT        #21=(CFSignal (CObList) "Last modified: 3-MAR-2011" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pUC19'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modified: 03/03/11 11:11" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 2686 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 25-OCT-2010" 1))) 
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COMMENT                                 "" 1))) "LMBP 5026") 
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COMMENT                                        "   complement(250..469)" 1))) 
COMMENT                                 "lacZ fragment, incl. lacZalpha") 
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COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "   complement(683..1085)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          "GAANNNNTTC" "CTTNNNNAAG") 
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COMMENT                          "GTYRAC" "CARYTG") 
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COMMENT                          "GAATTC" "CTTAAG") 
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COMMENT                          5 "AAGCTT" "TTCGAA") 
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COMMENT                                        " N1: 413 " 1))))) "KpnI: 1 site") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
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FEATURES             Location/Qualifiers
     CDS             complement(236..469)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     RBS             complement(470..507)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(508..591)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(683..1085)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(1626..2486)
                     /vntifkey="4"
                     /label=ampicillin\resistance
BASE COUNT      666 a       675 c       686 g       659 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct 
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
      661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
      721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
      781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
      841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
      901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
      961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
     1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 
     2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 
     2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc 
//
