ID pGEXGSTp65(12-317)S276C standard; circular DNA; ; 5872 BP. DE LMBP 5056. CC Accession number: LMBP 5056 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 10-NOV-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(2733..3593) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(1790..2192) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(1653..1730) FT /vntifkey="30" FT /label=lacIq\promoter,\incl.\RBS FT CDS complement(570..1652) FT /vntifkey="4" FT /label=lacI FT promoter complement(5657..5699) FT /vntifkey="30" FT /label=tac\promoter FT RBS complement(5621..5656) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT CDS complement(4962..5615) FT /vntifkey="4" FT /label=GST FT misc_marker complement(4938..4955) FT /vntifkey="22" FT /label=thrombin\recognition\site FT CDS complement(4020..4937) FT /vntifkey="4" FT /label=hNF-kappaB\p65\mutated\fragment FT CDS complement(7..447) FT /vntifkey="4" FT /label=lacZ\fragment,\incl.\lacZalpha FT RBS complement(448..485) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT promoter complement(486..569) FT /vntifkey="30" FT /label=lac\promoter FT mutation complement(4143..4145) FT /vntifkey="62" FT /label=S276C FT /note="WT: TCC" SQ Sequence 5872 BP; 1412 A; 1543 C; 1482 G; 1435 t; aattccaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac 60 attaaatgtg agcgagtaac aacccgtcgg attctccgtg ggaacaaacg gcggattgac 120 cgtaatggga taggttacgt tggtgtagat gggcgcatcg taaccgtgca tctgccagtt 180 tgaggggacg acgacagtat cggcctcagg aagatcgcac tccagccagc tttccggcac 240 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg 300 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 360 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 420 ggccagtgaa tccgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 480 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 540 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 600 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 660 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc 720 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc 780 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc 840 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc 900 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt 960 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc 1020 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca 1080 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc 1140 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca 1200 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc 1260 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc 1320 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca 1380 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc 1440 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg 1500 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca 1560 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac 1620 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc 1680 gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc ggaatttatg 1740 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 1800 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1860 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1920 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1980 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2040 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2100 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2160 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2220 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2280 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2340 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 2400 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 2460 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 2520 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 2580 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 2640 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 2700 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 2760 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 2820 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 2880 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2940 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 3000 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 3060 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 3120 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 3180 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 3240 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 3300 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 3360 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 3420 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 3480 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 3540 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 3600 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3660 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3720 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 3780 aggccctttc gtcttcaaga attatacact ccgctatcgc tacgtgactg ggtcatggct 3840 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3900 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3960 tcatcaccga aacgcgcgag gcagatcgtc agtcagtcac gatgaattcc cggggatcca 4020 ggactcttct tcatgatgct cttgaaggtc tcatatgtcc ttttacgttt ctcctcaatc 4080 cggtgacgat cgtctgtatc tggcaggtac tggaattcca tgggctcact gagctcccgg 4140 tcgcaaggcc tccgcagctg catggagaca cgcacaggag cctgcaggct ggggtctgcg 4200 tagggagggg tccggaacac aatggccact tgtcggtgca catcagcttg cgaaaaggag 4260 cctcgggcct cccagcctgg tcccgtgaaa tacacctcaa tgtcctcttt ctgcaccttg 4320 tcacacagta ggaagatctc atccccaccg aggcagctgc cagagtttcg gttcactcgg 4380 cagatcttga gctcggcagt gttgggggca cgattgtcaa agatgggatg aggaaggaca 4440 ggcggcaggc ggaggggcct gcctgatggg tcccgcactg tcacctggaa gcagagccgc 4500 acagcattca ggtcgtagtc cccacgctgc tcttctatag gaacttggaa ggggttgttg 4560 ttggtctgga tgcgctgact gatagcctgc tccaggtccc gcttcttcac acactggatt 4620 cccaggttct ggaaactgtg gatgcagcgg tccgggcaga gctcagcctc atagaagcca 4680 tcccggcagt cctttcctac aagctcgtgg gggtgaggcc ggtgaggagg gtccttggtg 4740 accagggaga tgcgcactgt ccctggtcct gtgtagccat tgatcttgat ggtggggtgg 4800 gtcttggtgg tatctgtgct cctctcgcct gggatgctgc ccgcggagcg cccctcgcac 4860 ttgtagcgga agcgcatgcc ccgctgcttg ggctgctcaa tgatctccac ataggggcca 4920 gaggcctggg ctggctcgga tccacgcgga accagatccg attttggagg atggtcgcca 4980 ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag 5040 tacttatcaa tttgtgggat agcttcaata cgttttttaa aacaaactaa ttttgggaac 5100 gcatccaggc acattgggtc catgtataaa acaacatcaa gagcgtcata caacatgaag 5160 tcaggatggg ttacatgatc accatttaaa tatgttttat gacataaacg atcttcgaac 5220 attttcagca tttcaggtag cttgctaaga aaatcaactt tgagagtttc aaagtcttta 5280 ctatatgcaa ttctcgaaac accgtatcta atatccaaaa ccgctccttc aagcattgaa 5340 atctctgcac gctcttttgg acaaccaccc aacatgttgt gcttgtcagc tatataacgt 5400 atgatggcca tagactgtgt taatttaaca tcaccatcaa tataataagg aagattggga 5460 aactccaaac ccaattcaaa ctttttgttt cgccatttat caccttcatc gcgctcatac 5520 aaatgctctt catatttttc ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg 5580 cccttaattt tccaataacc tagtataggg gacatgaata ctgtttcctg tgtgaaattg 5640 ttatccgctc acaattccac acattatacg agccgatgat taattgtcaa cagctcattt 5700 cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt 5760 gcgccttgag cgacacgaat tatgcagtga tttacgacct gcacagccat accacagctt 5820 ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgataac gt 5872 //