LOCUS       pGEXGSTp65(285-551)        5772 bp    DNA     circular     27-SEP-2010
DEFINITION  LMBP 5057.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5057
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 27-SEP-2010
            
COMMENT     VNTNAME|pGEXGSTp65(285-551)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT             "pGEX-5X1-compl" "" 0 0 1 4017 1 4017 1 4017 0 "" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "AccI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1932341874 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pGal4-p65f286-550" "" 0 0 1 809 4018 4826 1714 2522 0 "" "" "" "" 
COMMENT             "" "" (CObList) (CTerm 1 0 0 "BsaHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 2 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 1128620333 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pGEX-5X1-compl" "" 0 0 1 946 4827 5772 4027 4972 0 "" "" "" "" "" 
COMMENT             "" (CObList) (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AccI" "BamHI" "BssHII" "EcoRI" "NarI" "PvuI" "SspI" "XhoI" 
COMMENT         "XmnI" "BglI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5772 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 4276 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 4835 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 1 887 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4827 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 660 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 312 0 3177 0 4804 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3611 0 5607 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 4012 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 3406 0 5122 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 284 0 2927 0 0 "")) 
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COMMENT        #16=(CFSignal (CObList) "pMB1 ori" 33 0 1 1790 2192 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1790..2192)") 
COMMENT        #17=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 5521 5556 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5521..5556)") 
COMMENT        #18=(CFSignal (CObList) "GST" 4 0 1 4862 5515 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4862..5515)") 
COMMENT        #19=(CFSignal (CObList) "factor Xa recognition site" 22 0 1 4841 4852 0 
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COMMENT        #20=(CFSignal (CObList) "tac promoter" 30 0 1 5557 5599 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5557..5591)") 
COMMENT        #21=(CFSignal (CObList) "hNF-kappaB p65 fragment" 4 0 1 4028 4831 0 
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COMMENT        #22=(CFSignal (CObList) "lac promoter" 30 0 1 486 569 0 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 1 7 447 0 
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COMMENT        #24=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 448 485 0 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Modification Date in the Original DB: 23-SEP-2010" 1))) 
COMMENT                   "Standard Fields") 
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FEATURES             Location/Qualifiers
     CDS             complement(2733..3593)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(570..1652)
                     /vntifkey="4"
                     /label=lacI
     promoter        complement(1653..1730)
                     /vntifkey="30"
                     /label=lacIq\promoter,\incl.\RBS
     rep_origin      complement(1790..2192)
                     /vntifkey="33"
                     /label=pMB1\ori
     RBS             complement(5521..5556)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     CDS             complement(4862..5515)
                     /vntifkey="4"
                     /label=GST
     misc_marker     complement(4841..4852)
                     /vntifkey="22"
                     /label=factor\Xa\recognition\site
     promoter        complement(5557..5599)
                     /vntifkey="30"
                     /label=tac\promoter
     CDS             complement(4028..4831)
                     /vntifkey="4"
                     /label=hNF-kappaB\p65\fragment
     promoter        complement(486..569)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(448..485)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     CDS             complement(7..447)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
BASE COUNT     1399 a      1489 c      1496 g      1388 t 
ORIGIN
        1 aattccaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac 
       61 attaaatgtg agcgagtaac aacccgtcgg attctccgtg ggaacaaacg gcggattgac 
      121 cgtaatggga taggttacgt tggtgtagat gggcgcatcg taaccgtgca tctgccagtt 
      181 tgaggggacg acgacagtat cggcctcagg aagatcgcac tccagccagc tttccggcac 
      241 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg 
      301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 
      361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 
      421 ggccagtgaa tccgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
      481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 
      541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
      601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
      661 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc 
      721 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc 
      781 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc 
      841 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc 
      901 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt 
      961 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc 
     1021 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca 
     1081 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc 
     1141 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca 
     1201 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc 
     1261 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc 
     1321 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca 
     1381 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc 
     1441 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg 
     1501 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca 
     1561 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac 
     1621 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc 
     1681 gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc ggaatttatg 
     1741 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 
     1801 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     1861 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     1921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     1981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     2041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     2101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     2161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     2221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     2281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     2341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     2401 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     2461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     2521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     2581 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     2641 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     2701 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     2761 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     2821 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     2881 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     2941 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     3001 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 
     3061 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     3121 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     3181 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     3241 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     3301 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 
     3361 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     3421 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     3481 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     3541 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     3601 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     3661 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     3721 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 
     3781 aggccctttc gtcttcaaga attatacact ccgctatcgc tacgtgactg ggtcatggct 
     3841 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 
     3901 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 
     3961 tcatcaccga aacgcgcgag gcagatcgtc agtcagtcac gatgcggccg ctcgagtcgt 
     4021 caccccctta ggagctgatc tgactcagca gggctgagaa gtccatgtcc gcaatggagg 
     4081 agaagtcttc atctcctgaa aggaggccat tggggagccc cggggccccc agtggagcag 
     4141 gagctgggtc ggggggcctc tgggcccctg tcactaggcg agttatagcc tcagggtact 
     4201 ccatcagcat gggctcagtt gtgtgggggg ccacaggtat gccctggttc agcagctgct 
     4261 gaaactcgga gttgtcgacg gatgccaggt ctgtgaacac agctgggtct gtgctgttgc 
     4321 caagcaaggc ccccaggtct tcatcatcaa actgcagctg cagcagggcc tctgacagcg 
     4381 ttccttcccc agcctgggtg ggcttggggg caggtggggc cacagcctga ggagggcctg 
     4441 gggctaggac tgggacaggg gctggggcct gggccagagc tgataccatg gctggagcag 
     4501 gggcaggggc tggagcctgg ggcaggactt ggggaggggc cggggccaag gccgaggcct 
     4561 ggctgatctg cccagaagga aacaccatgg tgggaaactc atcatagttg atggtgctca 
     4621 gggatgacgt aaagggatag ggctggggtg ctggcttggg gacagaagct gagctgcggg 
     4681 aaggcacagc aatgcgtcga ggtggaggcc gggggtcggt gggtccgctg aaaggactct 
     4741 tcttcatgat gctcttgaag gtctcatatg tccttttacg tttctcctca atccggtgac 
     4801 gatcgtctgt atctggcagg tactggaatt cggggatccc acgaccttcg atcagatccg 
     4861 attttggagg atggtcgcca ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta 
     4921 tatacttgct ggatttcaag tacttatcaa tttgtgggat agcttcaata cgttttttaa 
     4981 aacaaactaa ttttgggaac gcatccaggc acattgggtc catgtataaa acaacatcaa 
     5041 gagcgtcata caacatgaag tcaggatggg ttacatgatc accatttaaa tatgttttat 
     5101 gacataaacg atcttcgaac attttcagca tttcaggtag cttgctaaga aaatcaactt 
     5161 tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac accgtatcta atatccaaaa 
     5221 ccgctccttc aagcattgaa atctctgcac gctcttttgg acaaccaccc aacatgttgt 
     5281 gcttgtcagc tatataacgt atgatggcca tagactgtgt taatttaaca tcaccatcaa 
     5341 tataataagg aagattggga aactccaaac ccaattcaaa ctttttgttt cgccatttat 
     5401 caccttcatc gcgctcatac aaatgctctt catatttttc ttcaagatat tccaaaagaa 
     5461 gtcgagtggg ttgcacaagg cccttaattt tccaataacc tagtataggg gacatgaata 
     5521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccgatgat 
     5581 taattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa 
     5641 acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga tttacgacct 
     5701 gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc 
     5761 agtcgataag ct 
//
