ID pGEXGSTp65(285-551) standard; circular DNA; ; 5772 BP. DE LMBP 5057. CC Accession number: LMBP 5057 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 27-SEP-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(2733..3593) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS complement(570..1652) FT /vntifkey="4" FT /label=lacI FT promoter complement(1653..1730) FT /vntifkey="30" FT /label=lacIq\promoter,\incl.\RBS FT rep_origin complement(1790..2192) FT /vntifkey="33" FT /label=pMB1\ori FT RBS complement(5521..5556) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT CDS complement(4862..5515) FT /vntifkey="4" FT /label=GST FT misc_marker complement(4841..4852) FT /vntifkey="22" FT /label=factor\Xa\recognition\site FT promoter complement(5557..5599) FT /vntifkey="30" FT /label=tac\promoter FT CDS complement(4028..4831) FT /vntifkey="4" FT /label=hNF-kappaB\p65\fragment FT promoter complement(486..569) FT /vntifkey="30" FT /label=lac\promoter FT RBS complement(448..485) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT CDS complement(7..447) FT /vntifkey="4" FT /label=lacZ\fragment,\incl.\lacZalpha SQ Sequence 5772 BP; 1399 A; 1489 C; 1496 G; 1388 t; aattccaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac 60 attaaatgtg agcgagtaac aacccgtcgg attctccgtg ggaacaaacg gcggattgac 120 cgtaatggga taggttacgt tggtgtagat gggcgcatcg taaccgtgca tctgccagtt 180 tgaggggacg acgacagtat cggcctcagg aagatcgcac tccagccagc tttccggcac 240 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg 300 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 360 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 420 ggccagtgaa tccgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 480 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 540 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 600 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 660 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc 720 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc 780 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc 840 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc 900 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt 960 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc 1020 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca 1080 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc 1140 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca 1200 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc 1260 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc 1320 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca 1380 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc 1440 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg 1500 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca 1560 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac 1620 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc 1680 gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc ggaatttatg 1740 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 1800 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1860 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1920 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1980 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2040 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2100 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2160 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2220 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2280 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2340 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 2400 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 2460 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 2520 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 2580 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 2640 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 2700 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 2760 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 2820 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 2880 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2940 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 3000 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 3060 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 3120 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 3180 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 3240 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 3300 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 3360 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 3420 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 3480 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 3540 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 3600 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3660 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3720 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 3780 aggccctttc gtcttcaaga attatacact ccgctatcgc tacgtgactg ggtcatggct 3840 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3900 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3960 tcatcaccga aacgcgcgag gcagatcgtc agtcagtcac gatgcggccg ctcgagtcgt 4020 caccccctta ggagctgatc tgactcagca gggctgagaa gtccatgtcc gcaatggagg 4080 agaagtcttc atctcctgaa aggaggccat tggggagccc cggggccccc agtggagcag 4140 gagctgggtc ggggggcctc tgggcccctg tcactaggcg agttatagcc tcagggtact 4200 ccatcagcat gggctcagtt gtgtgggggg ccacaggtat gccctggttc agcagctgct 4260 gaaactcgga gttgtcgacg gatgccaggt ctgtgaacac agctgggtct gtgctgttgc 4320 caagcaaggc ccccaggtct tcatcatcaa actgcagctg cagcagggcc tctgacagcg 4380 ttccttcccc agcctgggtg ggcttggggg caggtggggc cacagcctga ggagggcctg 4440 gggctaggac tgggacaggg gctggggcct gggccagagc tgataccatg gctggagcag 4500 gggcaggggc tggagcctgg ggcaggactt ggggaggggc cggggccaag gccgaggcct 4560 ggctgatctg cccagaagga aacaccatgg tgggaaactc atcatagttg atggtgctca 4620 gggatgacgt aaagggatag ggctggggtg ctggcttggg gacagaagct gagctgcggg 4680 aaggcacagc aatgcgtcga ggtggaggcc gggggtcggt gggtccgctg aaaggactct 4740 tcttcatgat gctcttgaag gtctcatatg tccttttacg tttctcctca atccggtgac 4800 gatcgtctgt atctggcagg tactggaatt cggggatccc acgaccttcg atcagatccg 4860 attttggagg atggtcgcca ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta 4920 tatacttgct ggatttcaag tacttatcaa tttgtgggat agcttcaata cgttttttaa 4980 aacaaactaa ttttgggaac gcatccaggc acattgggtc catgtataaa acaacatcaa 5040 gagcgtcata caacatgaag tcaggatggg ttacatgatc accatttaaa tatgttttat 5100 gacataaacg atcttcgaac attttcagca tttcaggtag cttgctaaga aaatcaactt 5160 tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac accgtatcta atatccaaaa 5220 ccgctccttc aagcattgaa atctctgcac gctcttttgg acaaccaccc aacatgttgt 5280 gcttgtcagc tatataacgt atgatggcca tagactgtgt taatttaaca tcaccatcaa 5340 tataataagg aagattggga aactccaaac ccaattcaaa ctttttgttt cgccatttat 5400 caccttcatc gcgctcatac aaatgctctt catatttttc ttcaagatat tccaaaagaa 5460 gtcgagtggg ttgcacaagg cccttaattt tccaataacc tagtataggg gacatgaata 5520 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccgatgat 5580 taattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa 5640 acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga tttacgacct 5700 gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc 5760 agtcgataag ct 5772 //