LOCUS       pFGPDAngls2a            8719 bp    DNA     circular     21-MAR-2013
DEFINITION  LMBP 5067.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5067
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-MAR-2013
            
            
COMMENT     VNTNAME|pFGPDAngls2a|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     terminator      complement(403..947)
                     /vntifkey="43"
                     /label=trpC\terminator
     CDS             complement(1106..3170)
                     /vntifkey="4"
                     /label=exon\3\of\A.\niger\gls2a
     CDS             complement(3225..3966)
                     /vntifkey="4"
                     /label=exon\2\of\A.\niger\gls2a
                     /note="signal sequence part 2: 3226-3233"
     CDS             complement(4031..4097)
                     /vntifkey="4"
                     /label=exon\1\of\A.\niger\gls2a
                     /note="signal sequence part 1"
     rep_origin      complement(6716..7118)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(6541..6624)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        complement(4360..6488)
                     /vntifkey="29"
                     /label=A.\nidulans\gpdA\promoter
     CDS             complement(7659..8519)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    complement(1025..1105)
                     /vntifkey="21"
                     /label=3'\UTR\of\A.\niger\gls2a
     misc_feature    complement(4098..4183)
                     /vntifkey="21"
                     /label=5'\UTR\of\A.\niger\gls2a
     intron          complement(3967..4030)
                     /vntifkey="15"
                     /label=intron\1\of\A.\niger\gls2a
     intron          complement(3171..3224)
                     /vntifkey="15"
                     /label=intron\2\of\A.\niger\gls2a
BASE COUNT     2143 a      2167 c      2271 g      2135 t         3 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctctagaaa gaaggattac 
      421 ctctaaacaa gtgtacctgt gcattctggg taaacgactc ataggagagt tgtaaaaaag 
      481 tttcggccgg cgtattgggt gttacggagc attcactagg caaccatgca tggttactat 
      541 tgtataccat cttagtagga antgatttcg aggtttatac ctacgatgaa tgtgtgtcct 
      601 gtaggcttga gagttcaagg aagaaacatg caattatctt tgcgaaccca ggngctggtg 
      661 acggaatttt catagtcaag ctatcagagt aaagaagagg agcatgtcaa agtacaatta 
      721 gagacaaata tatagtcgcg tggagccaag agcggattcc tcagtctcgt aggtctcttg 
      781 acgaccgttg atctgcttga tctcgtctcc cgaaaatgaa aatagctctg ctaagctatt 
      841 cttctcttcg ccggagcctg naaggcgtta ctaggttgca gtcaatgcat taatgcattg 
      901 cagatgagct gtatctggaa gaggtaaacc cgaaaacgcg ttttattgac ttgcttccta 
      961 ttctacgact ctagaggatc cccaattcgg ccaaaaaggc caactttttt tttttttttt 
     1021 tttttcataa ctaaatacca ttgatgtttg atatatgagc ctcttgcata tatgtatggg 
     1081 tgctcaatcc atatcctcgt ctagtctaaa actcaatccg ccatgtcttt ccaatgccga 
     1141 cattggggtt cttcaccacc gcatatgcgg ccttgccatc gggctggctg tggtactgca 
     1201 tagaggctgt cgaagccgcc gaagctccat cctcaatgac agtcacactg gtcttaccct 
     1261 gccattcctt aggaggatca actaccacca cccgctcaac acgaacgttg gccatggtct 
     1321 tcaagtactc ggccgtctta ggacccttgg tgccaacatc ctccgagacc agggcagact 
     1381 cctggaagcg gaaacggcgg tggatataag ctccacgctc atagtcgaac gtctcaccgt 
     1441 catccacgta gagagagcca tcggcttgac cgttcttatc caagaccaca acaagagtgt 
     1501 acggatccca tctcatcaag gcgctactgc ggcgaggacg gtccttgcgg gggattacat 
     1561 ggccaccctg catcagcaat ggcacagtct ccataggagc aggcaccgta tgccgctttc 
     1621 cggctccctg gtagacggtg tagtcaaagt agtcatagta cttttcgtcg tcagcaaggt 
     1681 aaatgtcggc cgtggtggcc tcctcggaga caacaggctt agcaagaaga ccggtggagc 
     1741 cgagataaag ctggtcgtca atggcaaagc cagcctcatc ccaagggtga gcgtagtact 
     1801 gcggcctcac gatcggcatt ccgttcacgg aagcttcgtg gaaggcagtg taccaggcgg 
     1861 ggagaagctg atacctcagg cggatagcct gggagatgat agaccggtgt ggctcggcaa 
     1921 tcagatacgg ctctctccgg cgcgtgtcaa tatgcgcgtg ggcccggaag aaggggtacc 
     1981 aaataccagc ttggtaccat ctggtcaaga gctccttgct agggttctgg aagaaaccgc 
     2041 ccacgtcagc accggcaaag gggaagcccg caatgccgtt gttcagaacc atagggatgg 
     2101 aagcggccaa gtgttcccaa gtagcctggt tatcacccgt ccacatagca gacatccgtt 
     2161 gagcaccagc ataatatgac cgtgtcagaa tgaaaggccg acggatctcg cccttcttgc 
     2221 gctctagaag ggcatcgtag gtggcattga ccagggtgat tccatgaacg ttatggatgt 
     2281 cacggtgctc ccagttgcca tgatgaaggt tatccttggg catcgtggtt tcgggaccgt 
     2341 tgaaaaccga gggctcgttc atgtcgttcc aaatgaagac attggacagc gtccccttga 
     2401 acttgtcaaa cttgaataag ctgacccacc atttgatggc ggcggggttg aaggtatcga 
     2461 tccagtgaga agagccaggc caacaccacc cgtcgtagat ctcaccgtcc ttgttcttag 
     2521 tggccaagtc tttgctcttc atttcttgga cgatgctgta cttgtcctgg ttcttgatgt 
     2581 gcgggtcaat gataacgacg agtttgcgct ccgactcatc gagctgctcc tccatgctga 
     2641 tcggatcggg gaaactgagt ggatcccagg tgaaatactt tctgtcatcg gtatattcga 
     2701 tgtccagcca gatgacatcg taggggatct ggtacttgtc aaagttgcga tcgacctcct 
     2761 tgacatcctc atcagtgatg tagttccagc ggcactggtg ataagcaatg gcaaaatgtt 
     2821 gaggcaactg agtgtagccg gtgagttcac cataggtctt gctgatttcc tgtggggtag 
     2881 gaccaaggaa aacgaacacg tcgagctggc cggactccga aaaccaatga ctctgggtgt 
     2941 cagtggtggc gcccacgccg agagcaagag ggttaggaga tgaggtagac ttgacaatgt 
     3001 ccacccaggt ctctgcagca ttcagccaga agacaccgac ggtggagtcc ttgcgatgtg 
     3061 cctgcatgaa tggaatagca ccatacaagg tcatgggact gctcagctcg tactcaaata 
     3121 catccgcatt gtacatgcgg tagggctctt cgtgattccc ttcaccacct ctgggacgct 
     3181 gtcagttctg cgagatggaa aatgtcactg cgcgactagc ttaccgagtt tcctttaagg 
     3241 agagagagtc agcatgctca ggaattccaa aaacatgctt gtagccaggg aaggtgatat 
     3301 ccaatcccac actctcggga cccctgggct tggtgtccgt gtttccacca aagctctcat 
     3361 cccaccaagt gctttcatct tcctgggttt gctgctcgcc ctcgccttcg acctctacct 
     3421 tagggcgcca atgctccatg ttaaggtagc ccttgttgtt caattgaacg tgggtttggc 
     3481 catccctctt gaagtcggcg ctaaaggggg cgtggcggat gacagcctcg aactggttgt 
     3541 ccggaccgta caagactctg gtaaagccag actcggtttc aggtctcaag gtcgcggttt 
     3601 tgctcaactc caggccacca accaacaccc atttctctgc ctcattgtag cgttccttgc 
     3661 gtgctttgct atcgtgtcga agctggatgt caccgttcat gcgcttttcc tcatcgacaa 
     3721 caactcgggc ggcgccggac tcgaggaagg agacaacgag aggcagcttg actttctcgt 
     3781 tgggggagac ggacttgaga atggttccgt gcaattggcc atccttgaac tggatggagg 
     3841 atgagtcgag ttcgtatggg gaggcccagg aggaaccttg ggcggcagca tcgtcggcga 
     3901 atgctctgtt ccgcttacag aagcccgatt gagaacatgt cttgaagttc tcgtgtttca 
     3961 cggtgactat gcgtcattgt tagatgtcaa ggagagcctc accagtagga gtgtgattgg 
     4021 ataggcttac ctccggggat gacaaggcat ccgagtagga tcaccaagga cagcagtagg 
     4081 gtccaacggt tggacattgg ccgagttccg gccatgatgg gagagctcag tagtcaatca 
     4141 gagacactca acctctgaaa gaagaactat cagcaaccac taggtaccgt gatgtctgct 
     4201 caagcggggt agctgttagt caagctgcga tgaagtggga aagctcgaac tgaaaggttc 
     4261 aaaggaataa gggatgggaa ggatggagta tggatgtagc aaagtactta cttaggggaa 
     4321 ataaaggttc ttggatggga agatgaatat actgaagatg ggaaaagaaa gagaaaagaa 
     4381 aagagcagct ggtggggaga gcaggaaaat atggcaacaa atgttggact gacgcaacga 
     4441 ccttgtcaac cccgccgaca caccgggcgg acagacgggg caaagctgcc taccagggac 
     4501 tgagggacct cagcaggtcg agtgcagagc accggatggg tcgactgcca gcttgtgttc 
     4561 ccggtctgcg ccgctggcca gctcctgagc ggcctttccg gtttcataca ccgggcaaag 
     4621 caggagaggc acgatatttg gacgccctac agatgccgga tgggccaatt agggagctta 
     4681 cgcgccgggt actcgctcta cctacttcgg agaaggtact atctcgtgaa tcttttacca 
     4741 gatcggaagc aattggactt ctgtacctag gttaatggca tgctatttcg ccgacggcta 
     4801 tacacccctg gcttcacatt ctccttcgct tactgccggt gattcgatga agctccatat 
     4861 tctccgatga tgcaatagat tcttggtcaa cgaggggcac accagccttt ccacttcggg 
     4921 gcggaggggc ggccggtccc ggattaataa tcatccactg cacctcagag ccgccagagc 
     4981 tgtctggcca gtggcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg 
     5041 ccagaaagag tcaccggtca ctgtacagag ctcacgagtt cgtcacattt ttctacaaat 
     5101 ggtggaggcg gcggatttta ggctcaagtc atgaccctct gggtcactcc agaatcagct 
     5161 aggtcaacga ataaggatga ttctatagga agatccaggc accggtcaac catgatctgg 
     5221 acagatttgg gagctcggta taagctctcc acctatctta ttctgtatag tttaggctta 
     5281 aagtttatcc aggagatgtt gctgaagtcg atttgagtcc acttcctcac tggtagctat 
     5341 acgactttga tggtcgttgt aggggctgta ttaggtctcg atcaaacaca aatagaatta 
     5401 aatggtactc gagtccactg aaggtggctt ctccgtcttc cgtagccgtg ccgaaatcct 
     5461 tacagcttgt gttgtgtgac ttttggttac gccgtctgac ttttgtggtg agctaactag 
     5521 agatcatgct atatctcctg atttaataca atgctcatca taacattcca cctggaactg 
     5581 ctagcaacgt ttgacttgca ttgtgcaacg ccctttgcag agctatcgga tgatcaatag 
     5641 tgccacgttc taaattcaac caacgcaggt gccccaagcc ttcgacatcc ggatgtattt 
     5701 cgaaaacctc atggcgattg cagtcctcag attcatgttc attccaatgc tcattggtga 
     5761 ataaaaggtt cacagggaat aagttcaaac tcgagatact tgagaatatt gaaagccaaa 
     5821 ggaccctcta tgctccaagc tagagtctca gcctggaaag caaatccaaa tgaagctatg 
     5881 ctacctccaa ttcctcatca tcttatctat aatacagagt cgaagaatat cctcttgaca 
     5941 ccgctccgtc ctccgacttc aataaggagc ttactcctcc ttgacaccac ccctccagtt 
     6001 cttctcggcg ttctggaggg aggccttgtc ggtcttgggc tggccctggc tgagaaagct 
     6061 gttggcagcc ttaaagggac gctggaggtc accagtcgct ggcttcccga agacgtggat 
     6121 cttaaccaga ttcgaaagcg ccttcagcgg atgatcgact ggatcagaag agcgttggtg 
     6181 tacttgaagt acagatgcat gacggccatc atgccaacgc ccatgaactg gctcttaatg 
     6241 agctggcgga actggccctt atcgtactcc atgttggtag ttgtgacagg acgaggctcc 
     6301 tcgccgcttc caagcggagc aggctcgacg tatttcagtg tcgaaagatc tgcagagatt 
     6361 acttcaagtc agccaactgc aaacagaata tcccgccaat agctttggga cgatgcaaga 
     6421 tataaacgaa aaagacgaac cgttcttctt attgatttga gcctgtgtgt agagatacaa 
     6481 gggaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     6541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     6601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     6661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     6721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     6781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     6841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     6901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     6961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     7021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     7081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     7141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     7201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     7261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     7321 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     7381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     7441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     7501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     7561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     7621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     7681 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     7741 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     7801 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     7861 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     7921 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     7981 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     8041 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     8101 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     8161 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     8221 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     8281 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     8341 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     8401 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     8461 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     8521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     8581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     8641 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 
     8701 atcacgaggc cctttcgtc 
//
