LOCUS       pFA6a-KAN-SpcnxP 5117 bp dsDNACircular
DEFINITION  LMBP 5069.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        5101..5117
                     /label=SP6\promoter
     promoter        complement(2757..2773)
                     /label=T7\promoter
     CDS             1165..1974
                     /label=KANMX
     CDS             complement(3790..4650)
                     /label=AMP
     rep_origin      complement(2847..3249)
                     /label=pMB1\ori
     terminator      1980..2177
                     /label=TEF1\terminator
     promoter        821..1164
                     /label=TEF1\promoter
     terminator      559..660
                     /label=ADH1\terminator
     promoter        49..522
                     /label=Spcnx\promoter
     CDS             2222..2563
                     /label=Spcnxf
     terminator      2564..2736
                     /label=Spcnx\terminator
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcctt gatacctaca 
       61 gttgttatgc gtcagactaa agcatattct aatgattgaa gattcaaaga ctacgagtat 
      121 agcgctttca aggctttatg actagtgata gtttgtaaac ttttatttta tctcattgtt 
      181 tgcattactc ttttttttta cttaatgggg agaacaatta aagccaaata cttagtatat 
      241 taaaatgcaa acgtttactg taaacatgtt actgcaacct tcattatagc aagacattgc 
      301 gctccgtaat tcacaaagta agaagtaaaa taaatataaa gatctaatgc aaatacaact 
      361 taccagatag gtctttcaaa tctaatcatc taagtttaat gcgatttgtt tttcgttccg 
      421 gaaccttctc cattattctt tcgattatgt ttgctgatcc atctcatcga tcaaagccgt 
      481 agtactacca aaacacacgc aaaaccgcgc accgtagtta tcgaattccg atgggcccgg 
      541 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt 
      601 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc 
      661 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc 
      721 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc 
      781 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac 
      841 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat 
      901 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc 
      961 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac 
     1021 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt 
     1081 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca 
     1141 catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga 
     1201 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 
     1261 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg 
     1321 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 
     1381 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 
     1441 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct 
     1501 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 
     1561 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 
     1621 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 
     1681 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc 
     1741 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 
     1801 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 
     1861 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 
     1921 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagta 
     1981 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg 
     2041 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca 
     2101 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa 
     2161 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt taaacgagct 
     2221 cgagtctaat caaattcttg agaagtttct tgacgtatat gatattatta aagctaaact 
     2281 tcctccaaat gttgccgaaa aagttgacta ctatgttgaa accatcatcg agactcctga 
     2341 aattgggatt gcaattgttg ccgttcttgg ctctttaacc gctgttattc ttacttgtta 
     2401 cttttatttc tttgcttcct cttccccggc ttctttatct actggaacta cggaagctga 
     2461 gaaagagcag caagagaagt ttaagcaaga gactgagact gagaagatag acgtttctta 
     2521 cgctcccgaa actgaatcac caactgcgaa gaatgaagac taatgaaact gctaaaatgt 
     2581 caaaagtttt tcttatttta agaatcgcat agaagttgca ataaactact tcctgttcct 
     2641 ttgtggactt ttgactgaag taaatgaata tatcaatata tatactcact aatacagcat 
     2701 attttgttta caaagtttga ctcaaagtag cgactccgcg gatctgccgg tctccctata 
     2761 gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc 
     2821 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     2881 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     2941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     3001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     3061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     3121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     3181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag 
     3241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     3301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     3361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     3421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     3481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     3541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     3601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     3661 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     3721 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     3781 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     3841 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     3901 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     3961 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     4021 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     4081 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     4141 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     4201 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     4261 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     4321 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     4381 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
     4441 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     4501 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     4561 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     4621 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     4681 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     4741 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 
     4801 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 
     4861 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 
     4921 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 
     4981 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga 
     5041 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg 
     5101 atttaggtga cactata 
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