ID pEF6mBclxLMycHis standard; circular DNA; ; 6467 BP. DE LMBP 5078. CC Accession number: LMBP 5078 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 6-JAN-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1a\promoter FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1a\promoter FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT CDS 1635..2333 FT /vntifkey="4" FT /label=mBclxL FT /note="mouse BclxL coding sequence" FT misc_marker 2349..2378 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT misc_marker 2394..2411 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 2440..2664 FT /vntifkey="43" FT /label=BGH\polyA FT /note="polyA signal of the bovine growth hormone" FT rep_origin 2710..3138 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 3143..3424 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 3381..3456 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 3529..3594 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT /note="synthetic prokaryotic EM7 promoter" FT CDS 3595..3993 FT /vntifkey="4" FT /label=blasticidin\resistance FT /note="antibiotic resistance gene; coding sequence" FT terminator 4151..4225 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(4151..4225) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(4539..4930) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5471..6331) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter 4364..4447 FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 6467 BP; 1458 A; 1634 C; 1763 G; 1612 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtaagcgg 1620 ccgcaatggc tagcatgtct cagagcaacc gggagctggt ggtcgacttt ctctcctaca 1680 agctttccca gaaaggatac agctggagtc agtttagtga tgtcgaagag aataggactg 1740 aggccccaga agaaactgaa gcagagaggg agacccccag tgccatcaat ggcaacccat 1800 cctggcacct ggcggatagc ccggccgtga atggagccac tggccacagc agcagtttgg 1860 atgcgcggga ggtgattccc atggcagcag tgaagcaagc gctgagagag gcaggcgatg 1920 agtttgaact gcggtaccgg agagcgttca gtgatctaac atcccagctt cacataaccc 1980 cagggaccgc gtatcagagc tttgagcagg tagtgaatga actctttcgg gatggagtaa 2040 actggggtcg catcgtggcc tttttctcct ttggcggggc actgtgcgtg gaaagcgtag 2100 acaaggagat gcaggtattg gtgagtcgga ttgcaagttg gatggccacc tatctgaatg 2160 accacctaga gccttggatc caggagaacg gcggctggga cacttttgtg gatctctacg 2220 ggaacaatgc agcagccgag agccggaaag gcaaggaggg cttcaaccgc tggtttctga 2280 cgggcatgac tgtggctggt gtggttctgc tgggctcact cttcagtcgg aagtctagag 2340 ggcccttcga acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc 2400 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 2460 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 2520 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 2580 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 2640 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 2700 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 2760 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 2820 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 2880 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 2940 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 3000 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 3060 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 3120 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 3180 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 3240 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3300 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 3360 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 3420 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 3480 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa 3540 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc 3600 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc 3660 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc 3720 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg 3780 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag 3840 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct 3900 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa 3960 ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg 4020 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 4080 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 4140 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 4200 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 4260 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 4320 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 4380 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 4440 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 4500 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 4560 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 4620 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 4680 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 4740 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 4800 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 4860 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 4920 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 4980 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5040 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5100 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 5160 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5220 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 5280 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 5340 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 5400 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 5460 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 5520 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 5580 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 5640 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 5700 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 5760 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 5820 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 5880 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 5940 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 6000 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 6060 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 6120 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 6180 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 6240 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 6300 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 6360 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 6420 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 6467 //