LOCUS       pRSV-RBP3-EEF238AAA 6623 bp dsDNACircular
DEFINITION  LMBP 5080.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        421..603
                     /label=RSV\LTR
     CDS             666..2135
                     /label=RBPm
     rep_origin      3161..3236
                     /label=SV40\ori
     CDS             3328..4122
                     /label=NEO
     CDS             complement(5627..6487)
                     /label=AMP
     promoter        complement(2177..2193)
                     /label=SP6\promoter
     terminator      2219..2443
                     /label=BGH\polyA
     rep_origin      2489..2917
                     /label=f1\ori
     promoter        2922..3204
                     /label=SV40\early\promoter
     terminator      4296..4370
                     /label=SV40\polyA\early
     terminator      complement(4296..4370)
                     /label=SV40\polyA\late
     rep_origin      complement(4684..5086)
                     /label=pMB1\ori
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag 
      241 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc 
      301 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaataca 
      361 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa 
      421 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca 
      481 acagacaggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agataattgt 
      541 atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca 
      601 cctccaagct tgcttacatt tgcttctgac acaactgtgt tcactagcaa cctcaaacag 
      661 acaccatggc cggatccgca tggattaaaa ggaaatttgg tgagcggcct ccacctaaac 
      721 gacttactag ggaagctatg cgaaattatt taaaagagcg aggggatcaa acagtactta 
      781 ttcttcatgc aaaagttgca cagaagtcat atggaaatga aaaaaggttt ttttgcccac 
      841 ctccttgtgt atatcttatg ggcagcggat ggaagaaaaa aaaagaacaa atggaacgcg 
      901 atggttgttc tgaacaagag tctcaaccgt gtgcatttat tgggatagga aatagtgacc 
      961 aagaaatgca gcagctaaac ttggaaggaa agaactattg cacagccaaa acattgtata 
     1021 tatctgactc agacaagcga aagcacttca ttttttctgt aaagatgttc tatggcaaca 
     1081 gtgatgacat tggtgtgttc ctcagcaagc ggataaaagt catctccaaa ccttccaaaa 
     1141 agaagcagtc attgaaaaat gctgacttat gcattgcctc aggaacaaag gtggctctgt 
     1201 ttaatcgact acgatcccag acagttagta ccagatactt gcatgtagaa ggaggtaatt 
     1261 ttcatgccag ttcacagcag tggggagcct tttttattca tctcttggat gatgatgaag 
     1321 cagaaggagc ggccgccaca gtccgagatg tctacatcca ttatggacaa acatgcaaac 
     1381 ttgtgtgctc agttactggc atggcactcc caagattgat aattatgaaa gttgataagc 
     1441 ataccgcatt attggatgca gatgatcctg tgtcacaact ccataaatgt gcattttacc 
     1501 ttaaggatac agaaagaatg tatttgtgcc tttctcaaga aagaataatt caatttcagg 
     1561 ccactccatg tccaaaagaa ccaaataaag agatgataaa tgatggcgct tcctggacaa 
     1621 tcattagcac agataaggca gagtatacat tttatgaggg aatgggccct gtccttgccc 
     1681 cagtcactcc tgtgcctgtg gtagagagcc ttcagttgaa tggcggtggg gacgtagcaa 
     1741 tgcttgaact tacaggacag aatttcactc caaatttacg agtgtggttt ggggatgtag 
     1801 aagctgaaac tatgtacagg tgtggagaga gtatgctctg tgtcgtccca gacatttctg 
     1861 cattccgaga aggttggaga tgggtccggc aaccagtcca ggttccagta actttggtcc 
     1921 gaaatgatgg aatcatttat tccaccagcc ttacctttac ctacacacca gaaccagggc 
     1981 cacggccaca ttgcagtgta gcaggagcaa tccttccagc caattcaagc caggtgcccc 
     2041 ctaacgaatc aaacacaaac agcgagggaa gttacacaaa cgccagcaca aattcaacca 
     2101 gtgtcacatc atctacagcc acagtggtat cctaaggatc cgaattcctc gagcatgcat 
     2161 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact 
     2221 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 
     2281 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 
     2341 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 
     2401 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 
     2461 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 
     2521 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     2581 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     2641 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     2701 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     2761 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     2821 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta 
     2881 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 
     2941 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc 
     3001 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 
     3061 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 
     3121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     3181 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 
     3241 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat 
     3301 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct 
     3361 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 
     3421 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 
     3481 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 
     3541 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 
     3601 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 
     3661 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 
     3721 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 
     3781 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 
     3841 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 
     3901 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 
     3961 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 
     4021 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 
     4081 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg 
     4141 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct 
     4201 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 
     4261 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg 
     4321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     4381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct 
     4441 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 
     4501 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 
     4561 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 
     4621 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
     4681 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     4741 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     4801 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     4861 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     4921 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     4981 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     5041 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg 
     5101 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     5161 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     5221 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     5281 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     5341 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     5401 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     5461 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     5521 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     5581 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     5641 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     5701 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     5761 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     5821 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     5881 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     5941 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     6001 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     6061 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     6121 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     6181 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     6241 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     6301 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     6361 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     6421 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     6481 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     6541 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     6601 cccgaaaagt gccacctgac gtc 
//
