ID pRcRSVp65S529A standard; circular DNA; ; 7087 BP. DE LMBP 5085. CC Collection number: LMBP 5085 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 5-DEC-2014 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 421..603 FT /vntifkey="29" FT /label=RSV-LTR FT terminator 2588..2812 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 2858..3313 FT /vntifkey="33" FT /label=f1\ori FT promoter 3390..3672 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 3629..3704 FT /vntifkey="33" FT /label=SV40\ori FT terminator 4764..4838 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4764..4838) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS 3796..4590 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin complement(5148..5550) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(6091..6951) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS 700..2355 FT /vntifkey="4" FT /label=mutated\hNF-kappaB\p65\subunit FT misc_feature 2356..2541 FT /vntifkey="21" FT /label=3'\UTR\of\hNF-kappaB\p65\subunit FT mutation 2275..2286 FT /vntifkey="62" FT /label=S529A FT /note="WT: GGC.CTC.CTT.TCA (AA526-529)" SQ Sequence 7087 BP; 1570 A; 1976 C; 1853 G; 1688 t; gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag 240 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc 300 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaataca 360 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa 420 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca 480 acagacaggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agataattgt 540 atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca 600 cctccaagct tgatatcgaa ttccggagtt ccatggctcg tctgtagtgc acgccgcggg 660 cccagctgcg accccggccc cgcccccggg accccggcca tggacgaact gttccccctc 720 atcttcccgg cagagccagc ccaggcctct ggcccctatg tggagatcat tgagcagccc 780 aagcagcggg gcatgcgctt ccgctacaag tgcgaggggc gctccgcggg cagcatccca 840 ggcgagagga gcacagatac caccaagacc caccccacca tcaagatcaa tggctacaca 900 ggaccaggga cagtgcgcat ctccctggtc accaaggacc ctcctcaccg gcctcacccc 960 cacgagcttg taggaaagga ctgccgggat ggcttctatg aggctgagct ctgcccggac 1020 cgctgcatcc acagtttcca gaacctggga atccagtgtg tgaagaagcg ggacctggag 1080 caggctatca gtcagcgcat ccagaccaac aacaacccct tccaagttcc tatagaagag 1140 cagcgtgggg actacgacct gaatgctgtg cggctctgct tccaggtgac agtgcgggac 1200 ccatcaggca ggcccctccg cctgccgcct gtccttcctc atcccatctt tgacaatcgt 1260 gcccccaaca ctgccgagct caagatctgc cgagtgaacc gaaactctgg cagctgcctc 1320 ggtggggatg agatcttcct actgtgtgac aaggtgcaga aagaggacat tgaggtgtat 1380 ttcacgggac caggctggga ggcccgaggc tccttttcgc aagctgatgt gcaccgacaa 1440 gtggccattg tgttccggac ccctccctac gcagacccca gcctgcaggc tcctgtgcgt 1500 gtctccatgc agctgcggcg gccttccgac cgggagctca gtgagcccat ggaattccag 1560 tacctgccag atacagacga tcgtcaccgg attgaggaga aacgtaaaag gacatatgag 1620 accttcaaga gcatcatgaa gaagagtcct ttcagcggac ccaccgaccc ccggcctcca 1680 cctcgacgca ttgctgtgcc ttcccgcagc tcagcttctg tccccaagcc agcaccccag 1740 ccctatccct ttacgtcatc cctgagcacc atcaactatg atgagtttcc caccatggtg 1800 tttccttctg ggcagatcag ccaggcctcg gccttggccc cggcccctcc ccaagtcctg 1860 ccccaggctc cagcccctgc ccctgctcca gccatggtat cagctctggc ccaggcccca 1920 gcccctgtcc cagtcctagc cccaggccct cctcaggctg tggccccacc tgcccccaag 1980 cccacccagg ctggggaagg aacgctgtca gaggccctgc tgcagctgca gtttgatgat 2040 gaagacctgg gggccttgct tggcaacagc acagacccag ctgtgttcac agacctggca 2100 tccgtcgaca actccgagtt tcagcagctg ctgaaccagg gcatacctgt ggccccccac 2160 acaactgagc ccatgctgat ggagtaccct gaggctataa ctcgcctagt gacaggggcc 2220 cagaggcccc ccgacccagc tcctgctcca ctgggggccc cggggctccc caatggtctc 2280 cttgcaggag atgaagactt ctcctccatt gcggacatgg acttctcagc cctgctgagt 2340 cagatcagct cctaaggggg tgacgcctgc cctccccaga gcactggttg caggggattg 2400 aagccctcca aaagcactta cggattctgg tggggtgtgt tccaactgcc cccaactttg 2460 tggatgtctt ccttggaggg gggagccata ttttattctt ttattgtcag tatctgtatc 2520 tctctctctt tttggaggtg cttaaggccg ctcgagcatg catctagagc tcgctgatca 2580 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 2640 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 2700 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 2760 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 2820 gcggaaagaa ccagctgggg ctcgaggggg gatccccacg cgccctgtag cggcgcatta 2880 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 2940 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 3000 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3060 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3120 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3180 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 3240 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 3300 acgtttacaa tttaaatatt tgcttataca atcttcctgt ttttggggct tttctgatta 3360 tcaaccgggg tgggtaccga gctcgaattc tgtggaatgt gtgtcagtta gggtgtggaa 3420 agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3480 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 3540 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 3600 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 3660 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 3720 ttttgcaaaa agctcccggg agcttggata tccattttcg gatctgatca agagacagga 3780 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 3840 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 3900 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 3960 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 4020 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 4080 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 4140 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 4200 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 4260 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 4320 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 4380 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 4440 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 4500 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 4560 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 4620 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 4680 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 4740 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 4800 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4860 tgtccaaact catcaatgta tcttatcatg tctggatccc gtcgacctcg agagcttggc 4920 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 4980 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 5040 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5100 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5160 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5220 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5280 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5340 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5400 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5460 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5520 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5580 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5640 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5700 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5760 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 5820 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 5880 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5940 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6000 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6060 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6120 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6180 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6240 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6300 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6360 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6420 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6480 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6540 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6600 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6660 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6720 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6780 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 6840 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 6900 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 6960 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7020 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7080 tgacgtc 7087 //