LOCUS       pEF1crmA-T291R          7628 bp    DNA     circular     19-SEP-2007
DEFINITION  LMBP 5104.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5104
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 19-SEP-2007
            
COMMENT     VNTDATE|445783869|
COMMENT     VNTNAME|pEF1crmA-T291R|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1crmA-T291R" 0 7628 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NdeI" "SspI" "NruI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3259 3300 1 7628 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1726 0 2135 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1749 0 3203 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 2596 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SspI" "aatatt" 4 0 7 4 0 1983 0 1989 0 2638 0 
COMMENT            3069 0 3077 0 3085 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NruI" "tcgcga" 4 0 3 331 0 2901 0 5277 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "hEF1alpha promoter" 29 0 0 508 1651 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "508..1651") 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1668..1684") 
COMMENT        #7=(CFSignal (CObList) "intron" 15 0 0 704 1642 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "704..1642") 
COMMENT        #8=(CFSignal (CObList) "5' UTR of crmA" 21 0 0 1754 2028 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1632..1906") 
COMMENT        #9=(CFSignal (CObList) "3' UTR of crmA" 21 0 0 3055 3200 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2933..3078") 
COMMENT        #10=(CFSignal (CObList) "V5 epitope" 22 0 0 3259 3300 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2574..2615") 
COMMENT        #11=(CFSignal (CObList) "His-tag" 22 0 0 3310 3327 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2625..2642") 
COMMENT        #12=(CFSignal (CObList) "bovine growth hormone polyA" 43 0 0 3356 3580 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2671..2895") 
COMMENT        #13=(CFSignal (CObList) "f1 ori" 33 0 0 3626 4054 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2941..3369") 
COMMENT        #14=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4059 4340 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3374..3655") 
COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 4297 4372 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3612..3687") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5434 5508 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4749..4823") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5434 5508 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4749..4823)") 
COMMENT        #18=(CFSignal (CObList) "ampicillin" 4 0 1 6754 7614 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6069..6929)") 
COMMENT        #19=(CFSignal (CObList) "pMB1 ori" 33 0 1 5822 6213 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5137..5528)") 
COMMENT        #20=(CFSignal (CObList) "crmA-T291R" 4 0 0 2029 3054 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2029..3054") 
COMMENT        #21=(CFSignal (CObList) "neomycin" 4 0 0 4464 5258 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4464..5258")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 19-SEP-2007" 2 0 0 0 0 0 
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COMMENT                          1) 
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COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 05/07/07 12:51" 1) 
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COMMENT                          "Last Modified: 09/19/07 11:06" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7628 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 7-MAY-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 5104") 
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COMMENT                   "Annotations") 
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COMMENT                          "Misc. Feature (2 total)") 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "   2574..2615" 1))) "V5 epitope") 
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COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                        "   508..1651" 1))) "hEF1alpha promoter") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                   "Feature Map") 
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COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1alpha\promoter
                     /note="Human elongation factor 1alpha promoter"
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     intron          704..1642
                     /vntifkey="15"
                     /label=intron
                     /note="human elongation factor 1 alpha intron"
     misc_feature    1754..2028
                     /vntifkey="21"
                     /label=5'\UTR\of\crmA
                     /note="5' UTR of the cowpox virus crmA cDNA"
     CDS             2029..3054
                     /vntifkey="4"
                     /label=crmA-T291R
                     /note="mutant cowpox virus crmA coding sequence"
     misc_feature    3055..3200
                     /vntifkey="21"
                     /label=3'\UTR\of\crmA
                     /note="3' UTR of the cowpox virus crmA cDNA"
     misc_marker     3259..3300
                     /vntifkey="22"
                     /label=V5\epitope
     misc_marker     3310..3327
                     /vntifkey="22"
                     /label=His-tag
     terminator      3356..3580
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
     rep_origin      3626..4054
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4059..4340
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4297..4372
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      5434..5508
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(5434..5508)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     CDS             complement(6754..7614)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="ampicillin resistance gene"
     CDS             4464..5258
                     /vntifkey="4"
                     /label=neomycin
                     /note="Tn5 antibiotic resistance gene"
     rep_origin      complement(5822..6213)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1814 a      1839 c      1968 g      2007 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 
     1741 tgtggtggaa ttcccatgga agaacgaaag tagtataaaa gtaataaaac aaaaaaaaga 
     1801 atataaaaaa tttatagcca ctttctttga ggactgtttt cctgaaggaa atgaacctct 
     1861 ggaattagta tacacgctag attattctca aactcctaat tatgacagac tacgtagact 
     1921 gtttatacaa gattgaaaat atatttcttt ttattgagtg gtggtagtta cggatatcta 
     1981 atattaatat tagactatct ctatcgtcac acaacaaaat cgattgccat ggatatcttc 
     2041 agggaaatcg catcttctat gaaaggagag aatgtattca tttctccacc gtcaatctcg 
     2101 tcagtattga caatactgta ttatggagct aatggatcca ctgctgaaca gctatcaaaa 
     2161 tatgtagaaa aggaggcgga caagaataag gatgatatct cattcaagtc catgaataaa 
     2221 gtatatgggc gatattctgc agtgtttaaa gattcctttt tgagaaaaat tggagataat 
     2281 ttccaaactg ttgacttcac tgattgtcgc actgtagatg cgatcaacaa gtgtgttgat 
     2341 atcttcactg aggggaaaat taatccacta ttggatgaac cattgtctcc agatacctgt 
     2401 ctcctagcaa ttagtgccgt atactttaaa gcaaaatggt tgatgccatt tgaaaaggaa 
     2461 tttaccagtg attatccctt ttacgtatct ccaacggaaa tggtagatgt aagtatgatg 
     2521 tctatgtacg gcgaggcatt taatcacgca tctgtaaaag aatcattcgg caacttttca 
     2581 atcatagaac tgccatatgt tggagatact agtatggtgg taattcttcc agacaatatt 
     2641 gatggactag aatccataga acaaaatcta acagatacaa attttaagaa atggtgtgac 
     2701 tctatggatg ctatgtttat cgatgtgcac attcccaagt ttaaggtaac aggctcgtat 
     2761 aatctggtgg atgcgctagt aaagttggga ctgacagagg tgttcggttc aactggagat 
     2821 tatagcaata tgtgtaattc agatgtgagt gtcgacgcta tgatccacaa aacgtatata 
     2881 gatgtcaatg aagagtatcg cgaagcggct gcagcaactt gtgcgctggt ggcagactgt 
     2941 gcatcaacag ttacaaatga gttctgtgca gatcatccgt tcatctatgt gattaggcat 
     3001 gtcgatggca aaattctttt cgttggtaga tattgctctc caacaactaa ttaaatcaca 
     3061 ttcttaatat tagaatatta gaatattata tagttaagat ttttactaat tggttaacca 
     3121 tttttttaaa aaaatagaaa aaaaacatgt tatattagcg agggtcgtta ttcttccaat 
     3181 tgcaattggt aagatgacgg ggaattctgc agatatccag cacagtggcg gccgctcgag 
     3241 tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg 
     3301 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg 
     3361 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 
     3421 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 
     3481 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 
     3541 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 
     3601 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     3661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     3721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     3781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     3841 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     3901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     3961 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     4021 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 
     4081 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     4141 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     4201 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     4261 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     4321 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     4381 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 
     4441 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     4501 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     4561 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     4621 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 
     4681 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     4741 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     4801 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     4861 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     4921 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     4981 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     5041 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     5101 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     5161 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     5221 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa 
     5281 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 
     5341 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     5401 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 
     5461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     5521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 
     5581 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     5641 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     5701 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     5761 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     5821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     5881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     5941 aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     6001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     6061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     6121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     6181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     6241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     6301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     6361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     6421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     6481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     6541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     6601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     6661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     6721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     6781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     6841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     6901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     6961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     7021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     7081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     7141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     7201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     7261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     7321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     7381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     7441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     7501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     7561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     7621 cctttttc 
//
