ID pEF1crmA-T291R standard; circular DNA; ; 7628 BP. DE LMBP 5104. CC Accession number: LMBP 5104 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 19-SEP-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1alpha\promoter FT /note="Human elongation factor 1alpha promoter" FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT intron 704..1642 FT /vntifkey="15" FT /label=intron FT /note="human elongation factor 1 alpha intron" FT misc_feature 1754..2028 FT /vntifkey="21" FT /label=5'\UTR\of\crmA FT /note="5' UTR of the cowpox virus crmA cDNA" FT CDS 2029..3054 FT /vntifkey="4" FT /label=crmA-T291R FT /note="mutant cowpox virus crmA coding sequence" FT misc_feature 3055..3200 FT /vntifkey="21" FT /label=3'\UTR\of\crmA FT /note="3' UTR of the cowpox virus crmA cDNA" FT misc_marker 3259..3300 FT /vntifkey="22" FT /label=V5\epitope FT misc_marker 3310..3327 FT /vntifkey="22" FT /label=His-tag FT terminator 3356..3580 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT rep_origin 3626..4054 FT /vntifkey="33" FT /label=f1\ori FT promoter 4059..4340 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4297..4372 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 5434..5508 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(5434..5508) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT CDS complement(6754..7614) FT /vntifkey="4" FT /label=ampicillin FT /note="ampicillin resistance gene" FT CDS 4464..5258 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene" FT rep_origin complement(5822..6213) FT /vntifkey="33" FT /label=pMB1\ori SQ Sequence 7628 BP; 1814 A; 1839 C; 1968 G; 2007 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 1740 tgtggtggaa ttcccatgga agaacgaaag tagtataaaa gtaataaaac aaaaaaaaga 1800 atataaaaaa tttatagcca ctttctttga ggactgtttt cctgaaggaa atgaacctct 1860 ggaattagta tacacgctag attattctca aactcctaat tatgacagac tacgtagact 1920 gtttatacaa gattgaaaat atatttcttt ttattgagtg gtggtagtta cggatatcta 1980 atattaatat tagactatct ctatcgtcac acaacaaaat cgattgccat ggatatcttc 2040 agggaaatcg catcttctat gaaaggagag aatgtattca tttctccacc gtcaatctcg 2100 tcagtattga caatactgta ttatggagct aatggatcca ctgctgaaca gctatcaaaa 2160 tatgtagaaa aggaggcgga caagaataag gatgatatct cattcaagtc catgaataaa 2220 gtatatgggc gatattctgc agtgtttaaa gattcctttt tgagaaaaat tggagataat 2280 ttccaaactg ttgacttcac tgattgtcgc actgtagatg cgatcaacaa gtgtgttgat 2340 atcttcactg aggggaaaat taatccacta ttggatgaac cattgtctcc agatacctgt 2400 ctcctagcaa ttagtgccgt atactttaaa gcaaaatggt tgatgccatt tgaaaaggaa 2460 tttaccagtg attatccctt ttacgtatct ccaacggaaa tggtagatgt aagtatgatg 2520 tctatgtacg gcgaggcatt taatcacgca tctgtaaaag aatcattcgg caacttttca 2580 atcatagaac tgccatatgt tggagatact agtatggtgg taattcttcc agacaatatt 2640 gatggactag aatccataga acaaaatcta acagatacaa attttaagaa atggtgtgac 2700 tctatggatg ctatgtttat cgatgtgcac attcccaagt ttaaggtaac aggctcgtat 2760 aatctggtgg atgcgctagt aaagttggga ctgacagagg tgttcggttc aactggagat 2820 tatagcaata tgtgtaattc agatgtgagt gtcgacgcta tgatccacaa aacgtatata 2880 gatgtcaatg aagagtatcg cgaagcggct gcagcaactt gtgcgctggt ggcagactgt 2940 gcatcaacag ttacaaatga gttctgtgca gatcatccgt tcatctatgt gattaggcat 3000 gtcgatggca aaattctttt cgttggtaga tattgctctc caacaactaa ttaaatcaca 3060 ttcttaatat tagaatatta gaatattata tagttaagat ttttactaat tggttaacca 3120 tttttttaaa aaaatagaaa aaaaacatgt tatattagcg agggtcgtta ttcttccaat 3180 tgcaattggt aagatgacgg ggaattctgc agatatccag cacagtggcg gccgctcgag 3240 tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg 3300 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg 3360 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 3420 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 3480 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 3540 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 3600 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 3660 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 3720 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 3780 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 3840 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 3900 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 3960 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 4020 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 4080 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 4140 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 4200 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 4260 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 4320 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 4380 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 4440 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 4500 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 4560 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 4620 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 4680 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 4740 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 4800 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 4860 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 4920 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 4980 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 5040 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 5100 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 5160 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 5220 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa 5280 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 5340 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 5400 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 5460 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 5520 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 5580 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 5640 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 5700 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 5760 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 5820 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 5880 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 5940 aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6000 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6060 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6120 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6180 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6240 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6300 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6360 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6420 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6480 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6540 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 6600 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 6660 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 6720 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 6780 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6840 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 6900 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6960 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7020 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 7080 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 7140 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7200 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7260 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7320 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 7380 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 7440 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 7500 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 7560 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7620 cctttttc 7628 //