ID pEF6crmA standard; circular DNA; ; 7270 BP. DE LMBP 5107. CC Accession number: LMBP 5107 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 2-FEB-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1a\promoter FT /note="human elongation factor 1alpha promoter" FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1a\promoter FT /note="human elongation factor 1alpha intron" FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT misc_feature 2951..3096 FT /vntifkey="21" FT /label=3'\UTR\of\crmA FT /note="3' UTR of the cowpox virus crmA cDNA" FT CDS 1925..2950 FT /vntifkey="4" FT /label=crmA FT /note="cowpox virus crmA" FT misc_feature 1632..1924 FT /vntifkey="21" FT /label=5'\UTR\of\crmA FT /note="5' UTR of the cowpox virus crmA cDNA" FT misc_marker 3152..3181 FT /vntifkey="22" FT /label=Myc\epitope FT promoter 4332..4397 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT CDS complement(6274..7134) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene" FT rep_origin 4184..4259 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 3946..4227 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin complement(5342..5733) FT /vntifkey="33" FT /label=pMB1\ori FT /note="Escherichia coli plasmid pMB1 origin" FT terminator complement(4954..5028) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 4954..5028 FT /vntifkey="43" FT /label=SV40\polyA\early FT CDS 4398..4796 FT /vntifkey="4" FT /label=blasticidin\resistance FT /note="antibiotic resistance gene" FT rep_origin 3513..3941 FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT terminator 3243..3467 FT /vntifkey="43" FT /label=BGH\polyA FT /note="polyA signal of the bovine growth hormone" FT misc_marker 3197..3214 FT /vntifkey="22" FT /label=His-tag FT promoter complement(5167..5250) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 7270 BP; 1792 A; 1708 C; 1836 G; 1934 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1620 tggtggaatt cccatggaag aacgaaagta gtataaaagt aataaaacaa aaaaaagaat 1680 ataaaaaatt tatagccact ttctttgagg actgttttcc tgaaggaaat gaacctctgg 1740 aattagttag atatatagaa ttagtataca cgctagatta ttctcaaact cctaattatg 1800 acagactacg tagactgttt atacaagatt gaaaatatat ttctttttat tgagtggtgg 1860 tagttacgga tatctaatat taatattaga ctatctctat cgtcacacaa caaaatcgat 1920 tgccatggat atcttcaggg aaatcgcatc ttctatgaaa ggagagaatg tattcatttc 1980 tccaccgtca atctcgtcag tattgacaat actgtattat ggagctaatg gatccactgc 2040 tgaacagcta tcaaaatatg tagaaaagga ggcggacaag aataaggatg atatctcatt 2100 caagtccatg aataaagtat atgggcgata ttctgcagtg tttaaagatt cctttttgag 2160 aaaaattgga gataatttcc aaactgttga cttcactgat tgtcgcactg tagatgcgat 2220 caacaagtgt gttgatatct tcactgaggg gaaaattaat ccactattgg atgaaccatt 2280 gtctccagat acctgtctcc tagcaattag tgccgtatac tttaaagcaa aatggttgat 2340 gccatttgaa aaggaattta ccagtgatta tcccttttac gtatctccaa cggaaatggt 2400 agatgtaagt atgatgtcta tgtacggcga ggcatttaat cacgcatctg taaaagaatc 2460 attcggcaac ttttcaatca tagaactgcc atatgttgga gatactagta tggtggtaat 2520 tcttccagac aatattgatg gactagaatc catagaacaa aatctaacag atacaaattt 2580 taagaaatgg tgtgactcta tggatgctat gtttatcgat gtgcacattc ccaagtttaa 2640 ggtaacaggc tcgtataatc tggtggatgc gctagtaaag ttgggactga cagaggtgtt 2700 cggttcaact ggagattata gcaatatgtg taattcagat gtgagtgtcg acgctatgat 2760 ccacaaaacg tatatagatg tcaatgaaga gtatacagaa gcagctgcag caacttgtgc 2820 gctggtggca gactgtgcat caacagttac aaatgagttc tgtgcagatc atccgttcat 2880 ctatgtgatt aggcatgtcg atggcaaaat tcttttcgtt ggtagatatt gctctccaac 2940 aactaattaa atcacattct taatattaga atattagaat attatatagt taagattttt 3000 actaattggt taaccatttt tttaaaaaaa tagaaaaaaa acatgttata ttagcgaggg 3060 tcgttattct tccaattgca attggtaaga tgacggggaa ttctgcagat atccagcaca 3120 gtggcggccg ctcgagtcta gagggccctt cgaacaaaaa ctcatctcag aagaggatct 3180 gaatatgcat accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc 3240 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 3300 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 3360 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 3420 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 3480 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc 3540 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 3600 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 3660 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 3720 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 3780 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 3840 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 3900 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt 3960 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4020 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 4080 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 4140 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 4200 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 4260 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 4320 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca 4380 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 4440 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 4500 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 4560 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 4620 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac 4680 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc 4740 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac 4800 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc 4860 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 4920 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 4980 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 5040 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 5100 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 5160 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 5220 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 5280 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 5340 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 5400 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 5460 aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5520 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5580 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5640 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5700 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5760 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5820 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5880 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5940 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6000 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6060 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 6120 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 6180 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 6240 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 6300 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6360 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 6420 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6480 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 6540 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 6600 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 6660 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6720 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6780 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 6840 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6900 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6960 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 7020 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 7080 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7140 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 7200 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 7260 acctgacgtc 7270 //