LOCUS       pEF6hBCL2               6581 bp    DNA     circular      6-MAY-2008
DEFINITION  LMBP 5109.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5109
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 6-MAY-2008
            
            
            
            
COMMENT     VNTDATE|481551655|
COMMENT     VNTNAME|pEF6hBCL2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6hBCL2" 0 6581 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "PvuII" "PsyI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "lac promoter" 
COMMENT             "GCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG" 
COMMENT             "from pUC18; 84 bp." 0 (CStringList "nagekeken 09/2004 MB") 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6581 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1604 0 2227 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1627 0 2410 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 2018 0 2801 0 3917 0 
COMMENT            4601 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PsyI" "gacnnngtc" 5 0 1 1857 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #6=(CFSignal (CObList) "intron hEF1alpha promoter" 15 0 0 582 1520 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #7=(CFSignal (CObList) "hEF1alpha promoter" 29 0 0 386 1529 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 0 2508 2525 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2508..2525") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 2824 3252 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2824..3252") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4265 4339 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4265..4339") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4265 4339 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4265..4339)") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 4653 5044 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4653..5044)") 
COMMENT        #13=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3257 3538 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3257..3538") 
COMMENT        #14=(CFSignal (CObList) "SV40 ori" 33 0 0 3495 3570 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3495..3570") 
COMMENT        #15=(CFSignal (CObList) "EM7 promoter" 30 0 0 3643 3708 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3643..3708") 
COMMENT        #16=(CFSignal (CObList) "Myc epitope" 22 0 0 2463 2492 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2463..2492") 
COMMENT        #17=(CFSignal (CObList) "lac promoter" 30 0 0 4478 4561 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "BGH polyA" 43 0 0 2554 2778 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2554..2778") 
COMMENT        #19=(CFSignal (CObList) "human BCL2" 4 0 0 1664 2383 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1664..2383") 
COMMENT        #20=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5585 6445 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5585..6445)") 
COMMENT        #21=(CFSignal (CObList) "blasticidin resistance" 4 0 0 3709 4107 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3709..4107")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "LMBP 5109" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 6-MAY-2008" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pEF6hBCL2'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5109." 
COMMENT                          1) 
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COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 05/05/08 12:20" 1) 
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COMMENT                          "Last Modified: 05/06/08 09:46" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6581 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 6-MAY-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 5109") 
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COMMENT                   "Annotations") 
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COMMENT                                 "intron hEF1alpha promoter"))) 
COMMENT                          "Intron (1 total)") 
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COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                   #80=(CLinePar 
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COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                        "   1546..1562" 1))) "T7 promoter") 
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COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                   "Feature Map") 
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COMMENT                            3 3 "CAGCTG" "GTCGAC"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1alpha\promoter
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1alpha\promoter
     CDS             1664..2383
                     /vntifkey="4"
                     /label=human\BCL2
     misc_marker     2508..2525
                     /vntifkey="22"
                     /label=His-tag
     terminator      2554..2778
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      2824..3252
                     /vntifkey="33"
                     /label=f1\ori
     CDS             3709..4107
                     /vntifkey="4"
                     /label=blasticidin\resistance
     terminator      4265..4339
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4265..4339)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4653..5044)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3257..3538
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3495..3570
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(5585..6445)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3643..3708
                     /vntifkey="30"
                     /label=EM7\promoter
     misc_marker     2463..2492
                     /vntifkey="22"
                     /label=Myc\epitope
     promoter        4478..4561
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1422 a      1736 c      1800 g      1623 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt cggcttgccc ccgttgcttt tcctctggga aggatggcgc acgctgggag 
     1681 aacggggtac gataaccggg agatagtgat gaagtacatc cattataagc tgtcgcagag 
     1741 gggctacgag tgggatgcgg gagatgtggg cgccgcgccc ccgggggccg cccccgcacc 
     1801 gggcatcttc tcctcccagc ccgggcacac gccccatcca gccgcatccc gcgacccggt 
     1861 cgccaggacc tcgccgctgc agaccccggc tgcccccggc gccgccgcgg ggcctgcgct 
     1921 cagcccggtg ccacctgtgg tccacctgac cctccgccag gccggcgacg acttctcccg 
     1981 ccgctaccgc cgcgacttcg ccgagatgtc cagccagctg cacctgacgc ccttcaccgc 
     2041 gcggggacgc tttgccacgg tggtggagga gctcttcagg gacggggtga actgggggag 
     2101 gattgtggcc ttctttgagt tcggtggggt catgtgtgtg gagagcgtca accgggagat 
     2161 gtcgcccctg gtggacaaca tcgccctgtg gatgactgag tacctgaacc ggcacctgca 
     2221 cacctggatc caggataacg gaggctggga tgcctttgtg gaactgtacg gccccagcat 
     2281 gcggcctctg tttgatttct cctggctgtc tctgaagact ctgctcagtt tggccctggt 
     2341 gggagcttgc atcaccctgg gtgcctatct gggccacaag tgaagtcaac atgcctgccc 
     2401 caaaagccga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggccct 
     2461 tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc 
     2521 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 
     2581 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 
     2641 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 
     2701 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 
     2761 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 
     2821 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     2881 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     2941 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     3001 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     3061 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     3121 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     3181 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     3241 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 
     3301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     3361 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3421 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     3481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     3541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     3601 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg 
     3661 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct 
     3721 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc 
     3781 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact 
     3841 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact 
     3901 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg 
     3961 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc 
     4021 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg 
     4081 ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacacgt 
     4141 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 
     4201 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 
     4261 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     4321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     4381 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     4441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     4501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     4561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     4621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     4681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     4741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccaggaacc 
     4801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     4861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     4921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     4981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     5041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     5101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     5161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     5221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 
     5281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     5341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     5401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     5461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     5521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     5581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     5641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     5701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     5761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     5821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     5881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     5941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     6001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     6061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     6121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     6181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     6241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     6301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     6361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     6421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     6481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     6541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c 
//
