ID pEF6hBCL2 standard; circular DNA; ; 6581 BP. DE LMBP 5109. CC Accession number: LMBP 5109 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 6-MAY-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1alpha\promoter FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1alpha\promoter FT CDS 1664..2383 FT /vntifkey="4" FT /label=human\BCL2 FT misc_marker 2508..2525 FT /vntifkey="22" FT /label=His-tag FT terminator 2554..2778 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 2824..3252 FT /vntifkey="33" FT /label=f1\ori FT CDS 3709..4107 FT /vntifkey="4" FT /label=blasticidin\resistance FT terminator 4265..4339 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4265..4339) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(4653..5044) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 3257..3538 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 3495..3570 FT /vntifkey="33" FT /label=SV40\ori FT CDS complement(5585..6445) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 3643..3708 FT /vntifkey="30" FT /label=EM7\promoter FT misc_marker 2463..2492 FT /vntifkey="22" FT /label=Myc\epitope FT promoter 4478..4561 FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 6581 BP; 1422 A; 1736 C; 1800 G; 1623 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1620 tggtggaatt cggcttgccc ccgttgcttt tcctctggga aggatggcgc acgctgggag 1680 aacggggtac gataaccggg agatagtgat gaagtacatc cattataagc tgtcgcagag 1740 gggctacgag tgggatgcgg gagatgtggg cgccgcgccc ccgggggccg cccccgcacc 1800 gggcatcttc tcctcccagc ccgggcacac gccccatcca gccgcatccc gcgacccggt 1860 cgccaggacc tcgccgctgc agaccccggc tgcccccggc gccgccgcgg ggcctgcgct 1920 cagcccggtg ccacctgtgg tccacctgac cctccgccag gccggcgacg acttctcccg 1980 ccgctaccgc cgcgacttcg ccgagatgtc cagccagctg cacctgacgc ccttcaccgc 2040 gcggggacgc tttgccacgg tggtggagga gctcttcagg gacggggtga actgggggag 2100 gattgtggcc ttctttgagt tcggtggggt catgtgtgtg gagagcgtca accgggagat 2160 gtcgcccctg gtggacaaca tcgccctgtg gatgactgag tacctgaacc ggcacctgca 2220 cacctggatc caggataacg gaggctggga tgcctttgtg gaactgtacg gccccagcat 2280 gcggcctctg tttgatttct cctggctgtc tctgaagact ctgctcagtt tggccctggt 2340 gggagcttgc atcaccctgg gtgcctatct gggccacaag tgaagtcaac atgcctgccc 2400 caaaagccga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggccct 2460 tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc 2520 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 2580 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 2640 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 2700 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 2760 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 2820 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 2880 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 2940 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 3000 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 3060 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 3120 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 3180 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 3240 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 3300 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 3360 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3420 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3480 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 3540 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 3600 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg 3660 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct 3720 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc 3780 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact 3840 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact 3900 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg 3960 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc 4020 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg 4080 ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacacgt 4140 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 4200 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 4260 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4320 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4380 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 4440 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 4500 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 4560 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 4620 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 4680 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4740 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccaggaacc 4800 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 4860 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 4920 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 4980 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 5040 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5100 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5160 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5220 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 5280 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5340 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5400 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5460 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5520 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5580 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5640 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 5700 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 5760 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 5820 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 5880 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 5940 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6000 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6060 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6120 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6180 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6240 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6300 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6360 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6420 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6480 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6540 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c 6581 //