LOCUS       pEF6-hXIAP              7589 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 5110.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5110
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 29-OCT-2007
COMMENT     VNTDATE|446049814|
COMMENT     VNTNAME|pEF6-hXIAP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hXIAP" 0 7589 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "Eco91I" "EcoRI" "HindIII" "PdmI" "SalI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7589 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "Eco91I" "ggtnacc" 2 0 1 2517 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1627 0 1910 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 4 651 0 3121 0 4261 0 
COMMENT            7266 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 3 266 0 1586 0 3276 0 0 
COMMENT            "") 
COMMENT        #4=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 5411 0 7588 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #6=(CFSignal (CObList) "hXIAP" 4 0 0 1934 3427 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "Myc epitope" 22 0 0 3471 3500 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1680..1709") 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 0 3516 3533 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1725..1742") 
COMMENT        #9=(CFSignal (CObList) "bovine growth hormone polyA" 43 0 0 3562 3786 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1771..1995") 
COMMENT        #10=(CFSignal (CObList) "f1 ori" 33 0 0 3832 4260 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2041..2469") 
COMMENT        #11=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4265 4546 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2474..2755") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 4503 4578 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2712..2787") 
COMMENT        #13=(CFSignal (CObList) "EM7 promoter" 30 0 0 4651 4716 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2860..2925") 
COMMENT        #14=(CFSignal (CObList) "blasticidin" 4 0 0 4717 5115 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2926..3324") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5273 5347 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3482..3556)") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5273 5347 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3482..3556") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 5661 6052 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3870..4261)") 
COMMENT        #18=(CFSignal (CObList) "ampicillin" 4 0 1 6593 7453 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4802..5662)") 
COMMENT        #19=(CFSignal (CObList) "hEF1alpha promoter" 29 0 0 386 1529 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #20=(CFSignal (CObList) "intron hEF1alpha promoter" 15 0 0 582 1520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "582..1520")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "LMBP 5110" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #24=(CRefLinePar 
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COMMENT                   "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA Plasmid 'pEF6-hXIAP'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5110." 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 05/10/07 02:43" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/29/07 11:00" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7589 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 10-MAY-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #37=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #40=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5110") 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 29-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1934 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1934  End: 3427" 1))) "hXIAP") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4717 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4717  End: 5115" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2926..3324" 1))) "blasticidin") 
COMMENT                            #51=(CFolderPar 
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COMMENT                                  (CParagraph 6593 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6593  End: 7453 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4802..5662)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (3 total)") 
COMMENT                     #55=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 582   End: 1520" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "intron of the human elongation factor 1alpha promoter" 
COMMENT                                        1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   582..1520" 1))) 
COMMENT                                 "intron hEF1alpha promoter"))) 
COMMENT                          "Intron (1 total)") 
COMMENT                     #61=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #62=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3471  End: 3500" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1680..1709" 1))) "Myc epitope") 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3516 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3516  End: 3533" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1725..1742" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #70=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 386 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 386   End: 1529" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Human elongation factor 1alpha promoter" 
COMMENT                                        1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   386..1529" 1))) "hEF1alpha promoter") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4265 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4265  End: 4546" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2474..2755" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #80=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1546 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1546  End: 1562" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1546..1562" 1))) "T7 promoter") 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4651 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4651  End: 4716" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2860..2925" 1))) "EM7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #89=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #90=(CFolderPar 
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COMMENT                                   #91=(CLinePar 
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COMMENT                                        "Start: 3832  End: 4260" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2041..2469" 1))) "f1 ori") 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4503 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4503  End: 4578" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2712..2787" 1))) "SV40 ori") 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5661 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5661  End: 6052 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(3870..4261)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #102=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3562  End: 3786" 1) 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1771..1995" 1))) 
COMMENT                                   "bovine growth hormone polyA") 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5273 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 5273  End: 5347 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3482..3556)" 1))) 
COMMENT                                   "SV40 polyA late") 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5273 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5273  End: 5347" 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3482..3556" 1))) 
COMMENT                                   "SV40 polyA early"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #115=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #116=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #118=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2517 " 1))))) "Eco91I: 1 site") 
COMMENT                            1 6 "GGTNACC" "CCANTGG") 
COMMENT                      #119=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 36496280 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                            " N1: 1627 " 1) 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1910 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                                     (CObjectList 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 266 " 1) 
COMMENT                                      #126=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1586 " 1) 
COMMENT                                      #127=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3276 " 1))))) 
COMMENT                             "HindIII: 3 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #128=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 21151088 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 651 " 1) 
COMMENT                                      #131=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3121 " 1) 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4261 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7266 " 1))))) "PdmI: 4 sites") 
COMMENT                            5 5 "GAANNNNTTC" "CTTNNNNAAG") 
COMMENT                      #134=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 35704096 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #136=(CLinePar 
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COMMENT                                            " N1: 5411 " 1) 
COMMENT                                      #137=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7588 " 1))))) "SalI: 2 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
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FEATURES             Location/Qualifiers
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1alpha\promoter
                     /note="intron of the human elongation factor 1alpha promoter"
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1alpha\promoter
                     /note="Human elongation factor 1alpha promoter"
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="Phage T7 gene 10 promoter"
     promoter        4651..4716
                     /vntifkey="30"
                     /label=EM7\promoter
                     /note="Synthetic prokaryotic EM7 promoter"
     misc_marker     3471..3500
                     /vntifkey="22"
                     /label=Myc\epitope
                     /note="Myc epitope"
     CDS             complement(6593..7453)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      4503..4578
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        4265..4546
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /note="Simian virus 40 early promoter"
     rep_origin      complement(5661..6052)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(5273..5347)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      5273..5347
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     CDS             4717..5115
                     /vntifkey="4"
                     /label=blasticidin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      3832..4260
                     /vntifkey="33"
                     /label=f1\ori
                     /note="Phage f1 origin"
     terminator      3562..3786
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
                     /note="Bovine growth hormone polyadenylation signal"
     misc_marker     3516..3533
                     /vntifkey="22"
                     /label=His-tag
                     /note="Histidine tag"
     CDS             1934..3427
                     /vntifkey="4"
                     /label=hXIAP
                     /note="Human X-linked inhibitor of apoptosis protein; coding sequence"
BASE COUNT     1880 a      1787 c      1961 g      1961 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ctgcagatgc ttcggtatcg ataagctatg gagcaaaagc tcatttctga 
     1681 agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga 
     1741 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga 
     1801 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagag 
     1861 cttgggcgac ctcaccatgg agcaaaagct catttctgaa gaggacttga attcctgcag 
     1921 cccgggggga tccatgactt ttaacagttt tgaaggatct aaaacttgtg tacctgcaga 
     1981 catcaataag gaagaagaat ttgtagaaga gtttaataga ttaaaaactt ttgctaattt 
     2041 tccaagtggt agtcctgttt cagcatcaac actggcacga gcagggtttc tttatactgg 
     2101 tgaaggagat accgtgcggt gctttagttg tcatgcagct gtagatagat ggcaatatgg 
     2161 agactcagca gttggaagac acaggaaagt atccccaaat tgcagattta tcaacggctt 
     2221 ttatcttgaa aatagtgcca cgcagtctac aaattctggt atccagaatg gtcagtacaa 
     2281 agttgaaaac tatctgggaa gcagagatca ttttgcctta gacaggccat ctgagacaca 
     2341 tgcagactat cttttgagaa ctgggcaggt tgtagatata tcagacacca tatacccgag 
     2401 gaaccctgcc atgtattgtg aagaagctag attaaagtcc tttcagaact ggccagacta 
     2461 tgctcaccta accccaagag agttagcaag tgctggactc tactacacag gtattggtga 
     2521 ccaagtgcag tgcttttgtt gtggtggaaa actgaaaaat tgggaacctt gtgatcgtgc 
     2581 ctggtcagaa cacaggcgac actttcctaa ttgcttcttt gttttgggcc ggaatcttaa 
     2641 tattcgaagt gaatctgatg ctgtgagttc tgataggaat ttcccaaatt caacaaatct 
     2701 tccaagaaat ccatccatgg cagattatga agcacggatc tttacttttg ggacatggat 
     2761 atactcagtt aacaaggagc agcttgcaag agctggattt tatgctttag gtgaaggtga 
     2821 taaagtaaag tgctttcact gtggaggagg gctaactgat tggaagccca gtgaagaccc 
     2881 ttgggaacaa catgctaaat ggtatccagg gtgcaaatat ctgttagaac agaagggaca 
     2941 agaatatata aacaatattc atttaactca ttcacttgag gagtgtctgg taagaactac 
     3001 tgagaaaaca ccatcactaa ctagaagaat tgatgatacc atcttccaaa atcctatggt 
     3061 acaagaagct atacgaatgg ggttcagttt caaggacatt aagaaaataa tggaggaaaa 
     3121 aattcagata tctgggagca actataaatc acttgaggtt ctggttgcag atctagtgaa 
     3181 tgctcagaaa gacagtatgc aagatgagtc aagtcagact tcattacaga aagagattag 
     3241 tactgaagag cagctaaggc gcctgcaaga ggagaagctt tgcaaaatct gtatggatag 
     3301 aaatattgct atcgtttttg ttccttgtgg acatctagtc acttgtaaac aatgtgctga 
     3361 agcagttgac aagtgtccca tgtgctacac agtcattact ttcaagcaaa aaatttttat 
     3421 gtcttaatct aactctatag tagctcgagc atgcatctag agggcccttc gaacaaaaac 
     3481 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt 
     3541 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     3601 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     3661 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     3721 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     3781 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 
     3841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     3901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     3961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     4021 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     4081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     4141 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     4201 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     4261 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     4321 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     4381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     4441 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     4501 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 
     4561 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 
     4621 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 
     4681 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa 
     4741 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac 
     4801 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta 
     4861 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg 
     4921 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc 
     4981 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg 
     5041 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat 
     5101 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt 
     5161 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 
     5221 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 
     5281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     5341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     5401 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 
     5461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     5521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     5581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     5641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     5701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     5761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg 
     5821 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     5881 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     5941 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     6001 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     6061 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     6121 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     6181 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     6241 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     6301 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     6361 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     6421 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     6481 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     6541 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     6601 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     6661 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     6721 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     6781 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     6841 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     6901 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     6961 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     7021 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     7081 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     7141 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     7201 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     7261 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     7321 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     7381 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     7441 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     7501 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     7561 catttccccg aaaagtgcca cctgacgtc 
//
