ID pEF6-hXIAP standard; circular DNA; ; 7589 BP. DE LMBP 5110. CC Accession number: LMBP 5110 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1alpha\promoter FT /note="intron of the human elongation factor 1alpha promoter" FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1alpha\promoter FT /note="Human elongation factor 1alpha promoter" FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT /note="Phage T7 gene 10 promoter" FT promoter 4651..4716 FT /vntifkey="30" FT /label=EM7\promoter FT /note="Synthetic prokaryotic EM7 promoter" FT misc_marker 3471..3500 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT CDS complement(6593..7453) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 4503..4578 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 4265..4546 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="Simian virus 40 early promoter" FT rep_origin complement(5661..6052) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator complement(5273..5347) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 5273..5347 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT CDS 4717..5115 FT /vntifkey="4" FT /label=blasticidin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 3832..4260 FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT terminator 3562..3786 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT /note="Bovine growth hormone polyadenylation signal" FT misc_marker 3516..3533 FT /vntifkey="22" FT /label=His-tag FT /note="Histidine tag" FT CDS 1934..3427 FT /vntifkey="4" FT /label=hXIAP FT /note="Human X-linked inhibitor of apoptosis protein; coding sequence" SQ Sequence 7589 BP; 1880 A; 1787 C; 1961 G; 1961 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1620 tggtggaatt ctgcagatgc ttcggtatcg ataagctatg gagcaaaagc tcatttctga 1680 agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga 1740 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga 1800 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagag 1860 cttgggcgac ctcaccatgg agcaaaagct catttctgaa gaggacttga attcctgcag 1920 cccgggggga tccatgactt ttaacagttt tgaaggatct aaaacttgtg tacctgcaga 1980 catcaataag gaagaagaat ttgtagaaga gtttaataga ttaaaaactt ttgctaattt 2040 tccaagtggt agtcctgttt cagcatcaac actggcacga gcagggtttc tttatactgg 2100 tgaaggagat accgtgcggt gctttagttg tcatgcagct gtagatagat ggcaatatgg 2160 agactcagca gttggaagac acaggaaagt atccccaaat tgcagattta tcaacggctt 2220 ttatcttgaa aatagtgcca cgcagtctac aaattctggt atccagaatg gtcagtacaa 2280 agttgaaaac tatctgggaa gcagagatca ttttgcctta gacaggccat ctgagacaca 2340 tgcagactat cttttgagaa ctgggcaggt tgtagatata tcagacacca tatacccgag 2400 gaaccctgcc atgtattgtg aagaagctag attaaagtcc tttcagaact ggccagacta 2460 tgctcaccta accccaagag agttagcaag tgctggactc tactacacag gtattggtga 2520 ccaagtgcag tgcttttgtt gtggtggaaa actgaaaaat tgggaacctt gtgatcgtgc 2580 ctggtcagaa cacaggcgac actttcctaa ttgcttcttt gttttgggcc ggaatcttaa 2640 tattcgaagt gaatctgatg ctgtgagttc tgataggaat ttcccaaatt caacaaatct 2700 tccaagaaat ccatccatgg cagattatga agcacggatc tttacttttg ggacatggat 2760 atactcagtt aacaaggagc agcttgcaag agctggattt tatgctttag gtgaaggtga 2820 taaagtaaag tgctttcact gtggaggagg gctaactgat tggaagccca gtgaagaccc 2880 ttgggaacaa catgctaaat ggtatccagg gtgcaaatat ctgttagaac agaagggaca 2940 agaatatata aacaatattc atttaactca ttcacttgag gagtgtctgg taagaactac 3000 tgagaaaaca ccatcactaa ctagaagaat tgatgatacc atcttccaaa atcctatggt 3060 acaagaagct atacgaatgg ggttcagttt caaggacatt aagaaaataa tggaggaaaa 3120 aattcagata tctgggagca actataaatc acttgaggtt ctggttgcag atctagtgaa 3180 tgctcagaaa gacagtatgc aagatgagtc aagtcagact tcattacaga aagagattag 3240 tactgaagag cagctaaggc gcctgcaaga ggagaagctt tgcaaaatct gtatggatag 3300 aaatattgct atcgtttttg ttccttgtgg acatctagtc acttgtaaac aatgtgctga 3360 agcagttgac aagtgtccca tgtgctacac agtcattact ttcaagcaaa aaatttttat 3420 gtcttaatct aactctatag tagctcgagc atgcatctag agggcccttc gaacaaaaac 3480 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt 3540 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 3600 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 3660 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 3720 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 3780 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 3840 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3900 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3960 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 4020 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 4080 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4140 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4200 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4260 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 4320 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 4380 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 4440 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 4500 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 4560 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 4620 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 4680 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa 4740 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac 4800 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta 4860 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg 4920 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc 4980 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg 5040 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat 5100 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt 5160 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 5220 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 5280 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 5340 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 5400 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 5460 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 5520 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 5580 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 5640 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 5700 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 5760 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg 5820 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 5880 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 5940 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6000 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6060 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6120 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 6180 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 6240 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 6300 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 6360 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 6420 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 6480 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 6540 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 6600 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 6660 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 6720 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 6780 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 6840 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 6900 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 6960 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7020 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7080 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7140 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7200 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7260 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7320 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7380 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 7440 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 7500 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 7560 catttccccg aaaagtgcca cctgacgtc 7589 //