LOCUS       pSP72-hABIN2            3732 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 5115.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTNAME|pSP72-hABIN2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSP72-hABIN2" 0 3732 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1 0 "" 0 "" "" "" "" "" "" "pSP72" "" 0 0 1 46 1 46 
COMMENT             1 46 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1 0 "" 0 "" "" "" "" "" "" "pCAGGS-E-hABIN-2" "" 0 
COMMENT             0 1 1289 47 1335 34 1322 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "SacI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 1 0 "" 0 "" "" "" "" "" "" "pSP72" "" 0 0 1 2397 
COMMENT             1336 3732 66 2462 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "SacI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " all enzymes of LMBP" 1 1 1 1 0 0 1 1 0 1 10 6 
COMMENT        4000 50 0 1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43) (CWordArray) 
COMMENT        (CStringList "BamHI" "ClaI" "EcoRI" "EcoRV" "HindII" "HindIII" "KpnI" 
COMMENT         "SacI" "SalI" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1 3732 1 3732 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 47 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 66 0 1338 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1352 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 17 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 35 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 41 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 1345 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 37 0 1407 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 76 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 1336 0 0 "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "T7 promoter" 30 0 1 1372 1388 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #14=(CFSignal (CObList) "SP6 promoter" 30 0 0 3716 3732 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #15=(CFSignal (CObList) "AMP" 4 0 1 2405 3265 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #16=(CFSignal (CObList) "pMB1 ori" 33 0 1 1462 1864 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #17=(CFSignal (CObList) "hABIN-2" 4 0 0 77 1336 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid pSP72-hABIN2" 0) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "collection number: 5115" 0) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3732 bp" 0) 
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COMMENT                          "storage type: Constructed" 0) 
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COMMENT                          "form: Circular" 0))) "General Description") 
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COMMENT                                        "  BamHI site #1" 0))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of pSP72 ") 
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COMMENT                                 "length: 1289" 0) 
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COMMENT                                        "  BamHI site #1" 0))) "Left Terminus") 
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COMMENT                                        "  SacI site #1" 0))) "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of pCAGGS-E-hABIN-2 ") 
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COMMENT                                 "length: 2397" 0) 
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COMMENT                                        "  End of Molecule" 0))) 
COMMENT                                 "Right Terminus"))) "#3: FRAGMENT of pSP72 "))) 
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COMMENT                                 "hABIN-2") 
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COMMENT                                 "AMP"))) "CDS (2 signals)") 
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COMMENT                                 "T7 promoter") 
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COMMENT                                 "SP6 promoter"))) 
COMMENT                          "Promoter_prokaryotic (2 signals)") 
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COMMENT                                   #68=(CLinePar 
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COMMENT                                 "pMB1 ori"))) "Rep_origin (1 signal)"))) 
COMMENT                   "Functional Map") 
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COMMENT                                        " N1: 47 " 0))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          "GATATC" "CTATAG") 
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COMMENT                                        " N1: 17 " 0))))) "HindIII: 1 site") 1 5 
COMMENT                          "AAGCTT" "TTCGAA") 
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COMMENT                                        " N1: 35 " 0))))) "SalI: 1 site") 1 5 
COMMENT                          "GTCGAC" "CAGCTG") 
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COMMENT                                        " N1: 41 " 0))))) "XbaI: 1 site") 1 5 
COMMENT                          "TCTAGA" "AGATCT") 
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COMMENT                                        " N1: 1345 " 0))))) "ClaI: 1 site") 2 4 
COMMENT                          "ATCGAT" "TAGCTA") 
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COMMENT                                        " N1: 76 " 0))))) "KpnI: 1 site") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
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COMMENT                          "GAGCTC" "CTCGAG") 
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COMMENT                          3 3 "GTYRAC" "CARYTG"))) "Restriction Map") 
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FEATURES             Location/Qualifiers
     promoter        complement(1372..1388)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        3716..3732
                     /vntifkey="30"
                     /label=SP6\promoter
     CDS             complement(2405..3265)
                     /vntifkey="4"
                     /label=AMP
     rep_origin      complement(1462..1864)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             77..1336
                     /vntifkey="4"
                     /label=hABIN-2
BASE COUNT      911 a       974 c      1043 g       804 t 
ORIGIN
        1 gaactcgagc agctgaagct tgcatgcctg caggtcgact ctagaggatc cgctggaacc 
       61 gcgtgaattc ggtaccatgt cccgggaccc ggggtcgggc ggctgggagg aggccccgcg 
      121 cgcagctgcc gcgctctgca ccctgtacca cgaggccgga cagcggctgc gccgcctgca 
      181 ggaccagctc gctgcccgcg acgccctcat cgctcgcctc cgcgcccgcc tggccgcgct 
      241 ggagggggac gccgcgccgt ccctagtgga cgcgctgctg gagcaggttg cgcgcttccg 
      301 ggagcagctg cgaaggcagg agggcggcgc cgccgaggcc cagatgcgcc aggaaattga 
      361 gaggctgact gagcgactag aagaaaaaga gagggagatg cagcagctgc tgagccagcc 
      421 ccaacacgag cgagagaagg aagtcgtcct gctacggagg agcatggcag aaggggagcg 
      481 cgcccgggcc gccagtgacg tcctgtgccg ctccttggcc aacgagaccc atcatctgcg 
      541 gaggacgctg accgccaccg cccacatgtg tcagcatctg gccaagtgtc tggatgaacg 
      601 acagcatgca caaaggaatg tgggggagag aagtcctgac cagtcggaac acacagatgg 
      661 gcacacctct gtccagagtg ttattgagaa gttgcaggaa gaaaatcgac tgttaaaaca 
      721 gaaggtgact cacgttgaag acctcaatgc caagtggcag cgctacaacg ccagcaggga 
      781 cgaatacgtg agggggctcc atgcgcagct cagggggctg cagatccccc acgagcccga 
      841 gctgatgagg aaggagatct cccggctcaa cagacagttg gaagagaaaa taaatgactg 
      901 tgccgaagtg aagcaggagc tggcggcctc caggacggcc cgggatgctg cgttggagcg 
      961 ggtgcagatg ctggaacagc agattctcgc ttacaaggat gacttcatgt cagaaagggc 
     1021 cgatcgggaa cgggctcaaa gtaggattca agaactggag gaaaaggtcg cctctttgct 
     1081 gcaccaggtg tcctggagac aggattctcg agagccagac gccggccgga ttcacgctgg 
     1141 gagcaaaact gccaagtatt tggccgccga cgcattagag cttatggtgc ctggtggctg 
     1201 gaggcctggg actgggtccc agcagccaga accccctgca gagggcgggc atcctggcgc 
     1261 ggcccagaga ggccaggggg accttcagtg ccctcactgc ctgcagtgct tcagtgacga 
     1321 gcaaggggaa gagctcgaat tcatcgatga tatcagatct gccggtctcc ctatagtgag 
     1381 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga 
     1441 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     1501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     1561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     1621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     1681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     1741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     1801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     1861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     1921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     1981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     2041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 
     2101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     2161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     2221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     2281 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     2341 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     2401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     2461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     2521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     2581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     2641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     2701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     2761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     2821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     2881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     2941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     3001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     3061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     3121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     3181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     3241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     3301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     3361 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
     3421 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 
     3481 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
     3541 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
     3601 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat 
     3661 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta 
     3721 ggtgacacta ta 
//
