LOCUS       pSilencer3.1-H1hygro        5033 bp    DNA     circular     20-DEC-2010
DEFINITION  LMBP 5118.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5118
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-DEC-2010
            
COMMENT     VNTDATE|572873543|
COMMENT     VNTNAME|pSilencer3.1-H1hygro|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "HindIII" "NdeI" "PstI" "RsaI" "SspI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 496 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1038 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 3 185 0 519 0 4053 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 4172 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "RsaI" "gtac" 3 0 5 170 0 725 0 2770 0 3535 0 
COMMENT            3588 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3094 0 4851 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "complement(577..1017)") 
COMMENT        #7=(CFSignal (CObList) "HA epitope" 22 0 1 535 561 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(535..561)") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 1 4579 4654 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2216 3076 0 
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COMMENT        #10=(CFSignal (CObList) "hygromycin resistance" 4 0 1 3480 4505 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3480..4505)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 1273 1675 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "H1 promoter" 29 0 0 402 494 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "SV40 early promoter" 29 0 1 4611 4847 0 
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COMMENT        #16=(CFSignal (CObList) "lac promoter" 30 0 1 1098 1181 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 1060 1097 0 
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COMMENT        #19=(CFSignal (CObList) "Last modified: 20-DEC-2010" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pSilencer3.1-H1hygro'" 1) 
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COMMENT                          "Created: 12/06/10 11:32" 1) 
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COMMENT                          "Last Modified: 12/20/10 05:09" 1) 
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COMMENT                          "Modification Date in the Original DB: 8-DEC-2010" 1))) 
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COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                 "pMB1 ori") 
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FEATURES             Location/Qualifiers
     CDS             complement(577..1017)
                     /vntifkey="4"
                     /label=GALbd
     misc_marker     complement(535..561)
                     /vntifkey="22"
                     /label=HA\epitope
     rep_origin      complement(4579..4654)
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(2216..3076)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(3480..4505)
                     /vntifkey="4"
                     /label=hygromycin\resistance
     rep_origin      complement(1273..1675)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        402..494
                     /vntifkey="29"
                     /label=H1\promoter
     promoter        complement(4611..4847)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     terminator      complement(3276..3350)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3276..3350
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(1098..1181)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(1060..1097)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
BASE COUNT     1232 a      1274 c      1280 g      1247 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catatttgca tgtcgctatg 
      421 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct 
      481 gtatgagacc actcggatcc ccggggcctc catggccata tgaccaccca agctagcgta 
      541 atctggaaca tcgtatgggt aagccatgaa ttccggcgat acagtcaact gtctttgacc 
      601 tttgttacta ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag 
      661 aggcatatca gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc 
      721 ttgtacaaat aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat 
      781 catgtcaagg tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct 
      841 tgattccact tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta 
      901 gcgacactcc cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt 
      961 gagcttttta agtcggcaaa tatcgcatgc ttgttcgata gaagacagta gcttcatctt 
     1021 tcaggaggct tgcttcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 
     1081 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 
     1141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     1201 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     1261 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     1321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     1381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     1441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     1501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     1561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     1621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     1681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     1741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     1801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     1861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 
     1921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     1981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     2041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     2101 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     2161 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     2221 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     2281 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     2341 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     2401 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     2461 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     2521 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     2581 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     2641 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     2701 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     2761 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     2821 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     2881 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     2941 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     3001 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     3061 aatgttgaat actcatactc ttcctttttc aatattcagg ctttacactt tatgcttccg 
     3121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 
     3181 catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg 
     3241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 
     3301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg 
     3361 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg 
     3421 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgc 
     3481 tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct 
     3541 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc 
     3601 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa 
     3661 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg 
     3721 agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc 
     3781 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg 
     3841 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg 
     3901 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc 
     3961 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc 
     4021 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga 
     4081 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg 
     4141 atcgcatcca tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg 
     4201 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat 
     4261 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca 
     4321 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct 
     4381 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag 
     4441 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt 
     4501 ttcatcacgt gctgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa 
     4561 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg 
     4621 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa 
     4681 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa 
     4741 ttgagatgca tgcttcgcat acttctgcct gctggggagc ctggggactt tccacaccag 
     4801 gttgctgact aattgagatg catgctttgc atacttctgc ctgctggaat attattgaag 
     4861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     4921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
     4981 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
