ID pSilencer3.1-H1hygro# standard; circular DNA; ; 5033 BP. DE LMBP 5118. CC Accession number: LMBP 5118 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 20-DEC-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(577..1017) FT /vntifkey="4" FT /label=GALbd FT misc_marker complement(535..561) FT /vntifkey="22" FT /label=HA\epitope FT rep_origin complement(4579..4654) FT /vntifkey="33" FT /label=SV40\ori FT CDS complement(2216..3076) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS complement(3480..4505) FT /vntifkey="4" FT /label=hygromycin\resistance FT rep_origin complement(1273..1675) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 402..494 FT /vntifkey="29" FT /label=H1\promoter FT promoter complement(4611..4847) FT /vntifkey="29" FT /label=SV40\early\promoter FT terminator complement(3276..3350) FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator 3276..3350 FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter complement(1098..1181) FT /vntifkey="30" FT /label=lac\promoter FT RBS complement(1060..1097) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS SQ Sequence 5033 BP; 1232 A; 1274 C; 1280 G; 1247 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catatttgca tgtcgctatg 420 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct 480 gtatgagacc actcggatcc ccggggcctc catggccata tgaccaccca agctagcgta 540 atctggaaca tcgtatgggt aagccatgaa ttccggcgat acagtcaact gtctttgacc 600 tttgttacta ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag 660 aggcatatca gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc 720 ttgtacaaat aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat 780 catgtcaagg tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct 840 tgattccact tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta 900 gcgacactcc cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt 960 gagcttttta agtcggcaaa tatcgcatgc ttgttcgata gaagacagta gcttcatctt 1020 tcaggaggct tgcttcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 1080 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 1140 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 1200 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 1260 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 1320 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1380 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 1440 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1500 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1560 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1620 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1680 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1740 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1800 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 1860 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 1920 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1980 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 2040 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 2100 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 2160 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 2220 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 2280 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 2340 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 2400 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 2460 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 2520 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 2580 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 2640 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 2700 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 2760 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 2820 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 2880 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 2940 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 3000 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 3060 aatgttgaat actcatactc ttcctttttc aatattcagg ctttacactt tatgcttccg 3120 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 3180 catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg 3240 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 3300 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg 3360 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg 3420 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgc 3480 tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct 3540 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc 3600 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa 3660 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg 3720 agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc 3780 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg 3840 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg 3900 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc 3960 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc 4020 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga 4080 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg 4140 atcgcatcca tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg 4200 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat 4260 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca 4320 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct 4380 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag 4440 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt 4500 ttcatcacgt gctgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa 4560 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg 4620 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa 4680 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa 4740 ttgagatgca tgcttcgcat acttctgcct gctggggagc ctggggactt tccacaccag 4800 gttgctgact aattgagatg catgctttgc atacttctgc ctgctggaat attattgaag 4860 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 4920 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 4980 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 5033 //