LOCUS       pGL3casp11p-Del8        4826 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 5188.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5188
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
COMMENT     VNTDATE|439300884|
COMMENT     VNTNAME|pGL3casp11p-Del8|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGL3casp11p-Del8" 0 4826 
COMMENT       (CDBMol 0 0 1 4733 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1668219001 0 "" 0 "" "" "" "" "" "" "pGL3-basic " 
COMMENT             "" 0 0 1 4747 95 15 87 15 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "NcoI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "MluI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 20037 0 "" 0 "" "" "" "" "" "" "PCR-Casp11p-Del8" 
COMMENT             "" 0 0 1 79 16 94 4 82 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "MluI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "NcoI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "MluI" "NcoI" "KpnI" "TatI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1579 1578 1 4826 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #2=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 16 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 95 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 6 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "TatI" "wgtacw" 2 0 5 260 0 587 0 1424 0 3640 0 
COMMENT            4723 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "LUCm" 4 0 0 96 1748 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "96..1748") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 0 1836 1932 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1836..1932") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 1 1836 1932 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1836..1932)") 
COMMENT        #9=(CFSignal (CObList) "ampicillin" 4 0 1 3088 3948 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3088..3948)") 
COMMENT        #10=(CFSignal (CObList) "f1 ori" 33 0 0 4080 4535 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4080..4535") 
COMMENT        #11=(CFSignal (CObList) "synthetic polyA" 43 0 0 4666 4819 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4666..4819") 
COMMENT        #12=(CFSignal (CObList) "48 bp mCASP-11 promoter fragm." 29 0 0 21 93 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "21..93") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 2145 2547 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2145..2547)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "LMBP 5188" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #15=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pGL3casp11p-Del8'" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5188." 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/28/07 12:01" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/29/07 11:58" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4826 bp" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 8-JUN-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #30=(CRefLinePar 
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COMMENT                                 "" 1))) "LMBP 5188") 
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COMMENT                                 "" 1))) "Last modified: 29-OCT-2007"))) 
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COMMENT                                 "parent position: from 87 to 15" 1) 
COMMENT                            #39=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 4747" 1) 
COMMENT                            #40=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 95 to 15" 1) 
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COMMENT                                        "  MluI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pGL3-basic%20?sel=87-15\">pGL3-basic </a> ") 
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COMMENT                                 "parent position: from 4 to 82" 1) 
COMMENT                            #47=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 79" 1) 
COMMENT                            #48=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
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COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/PCR-Casp11p-Del8?sel=4-82\">PCR-Casp11p-Del8</a> "))) 
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COMMENT                                        "Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm)" 
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COMMENT                                        "   96..1748" 1))) "LUCm") 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "   complement(3088..3948)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                                   #68=(CLinePar 
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COMMENT                                        "mCASP-11 -48/-1 promoter fragment (deletion8), incl. 25 bp mRNA" 
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COMMENT                                        "   21..93" 1))) 
COMMENT                                 "48 bp mCASP-11 promoter fragm."))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 2145  End: 2547 (Complementary)" 
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COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                 "pMB1 ori") 
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COMMENT                                        "origin phage f1" 1) 
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FEATURES             Location/Qualifiers
     CDS             96..1748
                     /vntifkey="4"
                     /label=LUCm
                     /note="Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm)"
     terminator      1836..1932
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      complement(1836..1932)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     CDS             complement(3088..3948)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      4080..4535
                     /vntifkey="33"
                     /label=f1\ori
                     /note="origin phage f1"
     terminator      4666..4819
                     /vntifkey="43"
                     /label=synthetic\polyA
                     /note="synthetic polyA signal"
     promoter        21..93
                     /vntifkey="29"
                     /label=48\bp\mCASP-11\promoter\fragm.
                     /note="mCASP-11 -48/-1 promoter fragment (deletion8), incl. 25 bp mRNA"
     rep_origin      complement(2145..2547)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
BASE COUNT     1258 a      1146 c      1155 g      1267 t 
ORIGIN
        1 ggtaccgagc tcttacgcgt ctatgctaag gaaatgccca gtaactttca ttttactctg 
       61 tcaagctgtc ttcacagtgc gaaagaactg aggccatgga agacgccaaa aacataaaga 
      121 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg 
      181 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg 
      241 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac 
      301 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct 
      361 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt 
      421 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt 
      481 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa 
      541 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca 
      601 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg 
      661 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg 
      721 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca 
      781 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa 
      841 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg 
      901 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc 
      961 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt 
     1021 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca 
     1081 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta 
     1141 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt 
     1201 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg 
     1261 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca 
     1321 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag 
     1381 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg 
     1441 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg 
     1501 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc 
     1561 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg 
     1621 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa 
     1681 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg 
     1741 ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg 
     1801 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 
     1861 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 
     1921 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt 
     1981 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc 
     2041 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 
     2101 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc 
     2161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     2221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     2281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     2341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     2401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     2461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     2521 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     2581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     2641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     2701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     2761 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     2821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     2881 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     2941 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     3001 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     3061 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     3121 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     3181 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     3241 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     3301 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     3361 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     3421 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     3481 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     3541 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     3601 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     3661 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     3721 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     3781 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     3841 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     3901 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     3961 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     4021 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     4081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     4141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     4201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     4261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     4321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     4381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     4441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     4501 cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca ggctgcgcaa 
     4561 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg 
     4621 ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta 
     4681 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc 
     4741 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca 
     4801 ggtgccagaa catttctcta tcgata 
//
