ID pSV-Sport-di-FR-HIFdel1 standard; circular DNA; ; 6021 BP. DE LMBP 5203. CC Accession number: LMBP 5203 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 83..1735 FT /vntifkey="4" FT /label=LUCm FT /note="Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc+)" FT CDS complement(4483..5343) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(3550..3952) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin of the plasmid" FT terminator complement(3341..3437) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT promoter 5641..5922 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="Simian virus 40 early promoter" FT terminator 3341..3437 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT intron 3065..3104 FT /vntifkey="15" FT /label=intron\SV40\small\t-antigen FT /note="intron of the SV40 small t-antigen" FT promoter complement(2960..2976) FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT promoter 5985..6001 FT /vntifkey="30" FT /label=SP6\promoter FT /note="Phage SP6 promoter" FT CDS 1933..2868 FT /vntifkey="4" FT /label=rLUC FT /note="Renilla reniformis luciferase gene (rLUC)" FT RBS 1743..1932 FT /vntifkey="32" FT /label=HIFdel1 FT /note="3' most half of the IRES of the mouse HIF-1alpha subunit" FT promoter 5539..5596 FT /vntifkey="30" FT /label=lacUV5\promoter FT rep_origin 5879..5954 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" SQ Sequence 6021 BP; 1621 A; 1380 C; 1421 G; 1599 t; cgagctctta cgcgtgctag cccgggctcg agatctgcga tctaagtaag cttggcattc 60 cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc 120 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata 180 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta 240 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa 300 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt 360 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga 420 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt 480 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga 540 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc 600 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc 660 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag 720 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc 780 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact 840 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt 900 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc 960 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc 1020 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct 1080 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc 1140 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt 1200 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag 1260 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga 1320 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt 1380 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga 1440 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc 1500 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat 1560 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg 1620 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag 1680 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta 1740 gacctaggaa cccgagccgg agctcagcga gcgcagcctg cagctcccgc ctcgccgtcc 1800 cggggggcgt cccgcctccc accccgcctc tggacttgtc tctttctccg cgcgcgcgga 1860 cagagcgggc gcttaggccc gagcgagccc gggggccgcc ggccgggaag acaacgcggg 1920 caccgattcg ccatggcttc gaaagtttat gatccagaac aaaggaaacg gatgataact 1980 ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat 2040 tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct 2100 tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca 2160 gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat 2220 cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt 2280 gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag 2340 atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg 2400 cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg 2460 gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca 2520 gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca 2580 ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt 2640 gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa 2700 tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact 2760 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga 2820 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataaga attcccgggt 2880 cgacgagctc actagtcggc ggccgctcta gaggatccaa gcttacgtac gcgtgcatgc 2940 gacgtcatag ctctctccct atagtgagtc gtattataag ctagcttggg atctttgtga 3000 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct 3060 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actagctgca tatgcttgct 3120 gcttgagagt tttgcttact gagtatgatt tatgaaaata ttatacacag gagctagtga 3180 ttctaattgt ttgtgtattt tagattcaca gtcccaaggc tcatttcagg cccctcagtc 3240 ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 3300 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 3360 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 3420 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 3480 cttatcatgt ctggatcctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3540 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3600 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 3660 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 3720 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 3780 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 3840 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 3900 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt 3960 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 4020 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 4080 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 4140 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcggccagt 4200 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4260 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4320 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4380 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 4440 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 4500 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 4560 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 4620 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 4680 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 4740 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 4800 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 4860 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 4920 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 4980 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 5040 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 5100 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 5160 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 5220 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 5280 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 5340 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 5400 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 5460 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 5520 gcgtatacga ggccctttca ctcattaggc accccaggct ttacacttta tagcttccgg 5580 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agcatcgatg 5640 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 5700 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 5760 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 5820 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta 5880 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 5940 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat 6000 agaaggtacg cctgcaggta c 6021 //