ID pSV-Sport-di-RF-UNR(d255-270) standard; circular DNA; ; 6181 BP. DE LMBP 5214. CC Accession number: LMBP 5214 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 1794..3446 FT /vntifkey="4" FT /label=LUCm FT /note="mutated firefly luciferase coding sequence" FT promoter 6080..6137 FT /vntifkey="30" FT /label=lacUV5\promoter FT promoter 1..282 FT /vntifkey="29" FT /label=SV40\early\promoter FT promoter 345..361 FT /vntifkey="30" FT /label=SP6\promoter FT CDS 398..1333 FT /vntifkey="4" FT /label=rLUC FT promoter complement(3501..3517) FT /vntifkey="30" FT /label=T7\promoter FT intron 3606..3645 FT /vntifkey="15" FT /label=intron\SV40\small\t-antigen FT terminator 3882..3978 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(3882..3978) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(4091..4493) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(5024..5884) FT /vntifkey="4" FT /label=ampicillin FT RBS 1378..1791 FT /vntifkey="32" FT /label=5'\UTR\(delta\255-270)\of\UNR\(IRES) FT rep_origin 239..314 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" SQ Sequence 6181 BP; 1709 A; 1371 C; 1429 G; 1672 t; ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 60 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 120 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 180 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta 240 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 300 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat 360 agaaggtacg cctgcaggta ccggtccgga attcgccatg gcttcgaaag tttatgatcc 420 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa 480 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat 540 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga 600 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg 660 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct 720 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca 780 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga 840 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc 900 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc 960 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga 1020 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg 1080 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga 1140 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga 1200 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca 1260 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa 1320 aaatgaacaa taagaattcc cgggtcgacg agctcactag tcggcggccg ctctagatgc 1380 tgcttatggc ggcgctggag agggggcgct gagctgttgg gtatgaagtg taacagaaca 1440 gactttacca cctgaaactg ctgcttcaag ttcagatcag gcaaggaaca aacctcgtaa 1500 caactaacaa gaccaaagaa gagtacactt aagttgaaga cacaacactt gatctgaaac 1560 aagaagtttg tgcctactca acagctttga aagagcactt cccaacgctg ctagtaagtg 1620 ctgtactgtg agattgcccg gtacagcagc agttgtattc tttattagct tggtagatca 1680 ttttctctcg ctcttttttt taatactagc aactttcatc ctttgaaacg tgtgctgaaa 1740 aagaagaatc agcaaatact actgaaagtg caatatttga gtatcactgc gccatggaag 1800 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg 1860 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta 1920 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt 1980 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg 2040 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg 2100 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc 2160 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc 2220 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt 2280 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc 2340 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg 2400 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca 2460 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat 2520 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg 2580 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc 2640 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca 2700 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag 2760 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca 2820 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg 2880 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg 2940 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa 3000 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca 3060 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta 3120 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca 3180 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg 3240 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg 3300 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac 3360 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca 3420 agaagggcgg aaagatcgcc gtgtaattct agaggatcca agcttacgta cgcgtgcatg 3480 cgacgtcata gctctctccc tatagtgagt cgtattataa gctagcttgg gatctttgtg 3540 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc 3600 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactagctgc atatgcttgc 3660 tgcttgagag ttttgcttac tgagtatgat ttatgaaaat attatacaca ggagctagtg 3720 attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag gcccctcagt 3780 cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt tttacttgct 3840 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 3900 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3960 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 4020 tcttatcatg tctggatcct gcattaatga atcggccaac gcgcggggag aggcggtttg 4080 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4140 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4200 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4260 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4320 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4380 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4440 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4500 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4560 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4620 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4680 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcggccag 4740 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4800 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4860 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4920 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 4980 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 5040 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 5100 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 5160 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 5220 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 5280 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 5340 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 5400 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 5460 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 5520 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 5580 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 5640 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 5700 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 5760 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 5820 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 5880 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 5940 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 6000 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 6060 ggcgtatacg aggccctttc actcattagg caccccaggc tttacacttt atagcttccg 6120 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagcatcgat 6180 g 6181 //