ID pSV-Sport-di-RF standard; circular DNA; ; 5787 BP. DE LMBP 5253. CC Accession number: LMBP 5253 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin 239..314 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 345..361 FT /vntifkey="30" FT /label=SP6\promoter FT /note="Phage SP6 promoter" FT promoter 1..282 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="Simian virus 40 early promoter" FT CDS 398..1333 FT /vntifkey="4" FT /label=rLUC FT /note="Renilla reniformis luciferase gene" FT CDS 1400..3052 FT /vntifkey="4" FT /label=LUCm FT /note="Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm)" FT promoter 5686..5743 FT /vntifkey="30" FT /label=lacUV5\promoter FT /note="Escherichia coli lac operon promoter; mutant (lacUV5)" FT rep_origin complement(3697..4099) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator complement(3488..3584) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 3488..3584 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT intron 3212..3251 FT /vntifkey="15" FT /label=intron\SV40\small\t-antigen FT /note="intron SV40 small t-antigen" FT CDS complement(4630..5490) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter complement(3107..3123) FT /vntifkey="30" FT /label=T7\promoter FT /note="Phage T7 gene 10 promoter" SQ Sequence 5787 BP; 1590 A; 1286 C; 1342 G; 1569 t; ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 60 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 120 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 180 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta 240 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 300 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat 360 agaaggtacg cctgcaggta ccggtccgga attcgccatg gcttcgaaag tttatgatcc 420 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa 480 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat 540 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga 600 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg 660 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct 720 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca 780 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga 840 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc 900 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc 960 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga 1020 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg 1080 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga 1140 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga 1200 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca 1260 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa 1320 aaatgaacaa taagaattcc cgggtcgaga tctgcgatct aagtaagctt ggcattccgg 1380 tactgttggt aaagccacca tggaagacgc caaaaacata aagaaaggcc cggcgccatt 1440 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc 1500 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc 1560 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac 1620 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg 1680 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt 1740 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca 1800 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc 1860 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc 1920 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact 1980 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac 2040 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga 2100 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg 2160 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct 2220 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt 2280 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc 2340 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc 2400 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga 2460 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt 2520 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg 2580 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa 2640 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat 2700 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt 2760 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga 2820 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac 2880 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg 2940 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa 3000 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aattctagag 3060 gatccaagct tacgtacgcg tgcatgcgac gtcatagctc tctccctata gtgagtcgta 3120 ttataagcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 3180 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 3240 gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag tatgatttat 3300 gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag attcacagtc 3360 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata 3420 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 3480 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 3540 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 3600 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcctgcat taatgaatcg 3660 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 3720 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 3780 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 3840 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3900 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3960 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 4020 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 4080 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4140 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4200 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4260 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 4320 ggacagtatt tggtatctgc ggccagttac cttcggaaaa agagttggta gctcttgatc 4380 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 4440 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 4500 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 4560 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 4620 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 4680 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 4740 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 4800 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 4860 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 4920 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 4980 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 5040 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5100 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 5160 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5220 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 5280 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5340 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5400 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5460 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 5520 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5580 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 5640 tatcatgaca ttaacctata aaaataggcg tatacgaggc cctttcactc attaggcacc 5700 ccaggcttta cactttatag cttccggctc gtataatgtg tggaattgtg agcggataac 5760 aatttcacac aggaaacagc atcgatg 5787 //