LOCUS       pSV-Sport-di-RF-UNR(1-390)        6141 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 5258.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5258
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
COMMENT     VNTDATE|454330006|
COMMENT     VNTNAME|pSV-Sport-di-RF-UNR(1-390)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSV-Sport-di-RF-UNR(1-390)" 0 6141 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) (CStringList "AflIII" "BanII" "HindIII" "NcoI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1378 1751 1 6141 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1373 0 3409 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 232 0 397 0 1753 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3421 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BanII" "grgcyc" 6 0 2 1355 0 2779 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AflIII" "acrygt" 2 0 5 581 0 1744 0 2248 0 
COMMENT            3431 0 4175 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "SP6 promoter" 30 0 0 345 361 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "345..361") 
COMMENT        #6=(CFSignal (CObList) "SV40 early promoter" 29 0 0 1 282 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1..282") 
COMMENT        #7=(CFSignal (CObList) "rLUC" 4 0 0 398 1333 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "398..1333") 
COMMENT        #8=(CFSignal (CObList) "LUCm" 4 0 0 1754 3406 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1754..3406") 
COMMENT        #9=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 6040 6097 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6040..6097") 
COMMENT        #10=(CFSignal (CObList) "T7 promoter" 30 0 1 3461 3477 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3461..3477)") 
COMMENT        #11=(CFSignal (CObList) "intron SV40 small t-antigen" 15 0 0 3566 3605 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3566..3605") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3842 3938 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3842..3938") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3842 3938 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3842..3938)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 4051 4453 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4051..4453)") 
COMMENT        #15=(CFSignal (CObList) "ampicillin" 4 0 1 4984 5844 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4984..5844)") 
COMMENT        #16=(CFSignal (CObList) "5' UTR (fragm. 1-390) of UNR (IRES)" 32 0 0 
COMMENT             1378 1751 0 (CStringList) (CStringList) 1 1 1 1 "1378..1751") 
COMMENT        #17=(CFSignal (CObList) "SV40 ori" 33 0 0 239 314 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "239..314")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 5258" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #20=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #21=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pSV-Sport-di-RF-UNR(1-390)" 2) 5 "" 0 4) 
COMMENT              #22=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pSV-Sport-di-RF-UNR(1-390)'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5258." 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: margotbaetens" 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/10/07 10:46" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/29/07 03:49" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6141 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 10-AUG-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "margotbaetens" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:margot.baetens@dmbr.ugent.be\">margot.baetens@dmbr.ugent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #37=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5258") 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #19# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 29-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 398 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 398   End: 1333" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   398..1333" 1))) "rLUC") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1754 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1754  End: 3406" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mutated firefly luciferase coding sequence" 
COMMENT                                        1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1754..3406" 1))) "LUCm") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4984 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4984  End: 5844 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4984..5844)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (3 total)") 
COMMENT                     #58=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3566 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3566  End: 3605" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "intron of the SV40 small t-antigen" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3566..3605" 1))) 
COMMENT                                 "intron SV40 small t-antigen"))) 
COMMENT                          "Intron (1 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 1 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 282 " 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..282" 1))) "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 345 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 345   End: 361 " 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   345..361" 1))) "SP6 promoter") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3461 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3461  End: 3477 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3461..3477)" 1))) 
COMMENT                                 "T7 promoter") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6040 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6040  End: 6097" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6040..6097" 1))) "lacUV5 promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
COMMENT                     #82=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1378 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1378  End: 1751" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1378..1751" 1))) 
COMMENT                                 "5' UTR (fragm. 1-390) of UNR (IRES)"))) 
COMMENT                          "RBS (1 total)") 
COMMENT                     #87=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 239 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 239   End: 314 " 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bidirectional origin of SV40" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   239..314" 1))) "SV40 ori") 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4051 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4051  End: 4453 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4051..4453)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #97=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3842 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3842  End: 3938" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "polyA signal of the SV40 early region" 
COMMENT                                         1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   3842..3938" 1))) "SV40 polyA early") 
COMMENT                            #103=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 3842 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3842  End: 3938 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "polyA signal of the SV40 late region" 
COMMENT                                          1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(3842..3938)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #108=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #109=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 25745896 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #110=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #111=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 581 " 1) 
COMMENT                                      #112=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1744 " 1) 
COMMENT                                      #113=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2248 " 1) 
COMMENT                                      #114=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3431 " 1) 
COMMENT                                      #115=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 4175 " 1))))) 
COMMENT                             "AflIII: 5 sites") 1 5 "ACRYGT" "TGYRCA") 
COMMENT                      #116=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24858656 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #117=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #118=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1355 " 1) 
COMMENT                                      #119=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2779 " 1))))) "BanII: 2 sites") 
COMMENT                            5 1 "GRGCYC" "CYCGRG") 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24901224 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #121=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3421 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #123=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24838376 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #124=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 232 " 1) 
COMMENT                                      #126=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 397 " 1) 
COMMENT                                      #127=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1753 " 1))))) "NcoI: 3 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #128=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 21877352 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #129=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #130=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1373 " 1) 
COMMENT                                      #131=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3409 " 1))))) "XbaI: 2 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #132=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #133=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #134=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #135=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #136=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #137=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #138=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #139=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #140=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #141=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #142=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #143=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #144=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #145=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #146=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #147=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #148=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #149=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #150=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #151=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #152=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #153=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #154=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #155=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #156=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #157=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #158=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #159=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #160=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #161=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #162=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #163=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #164=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #166=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #167=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #169=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #170=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #171=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #172=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #173=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #174=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #175=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #181=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #182=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #183=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #184=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #185=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #186=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #187=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #188=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #189=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #190=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #191=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #192=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #193=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #194=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #195=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #196=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #197=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #198=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #199=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #200=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #201=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #202=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #203=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #204=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #205=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #206=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #207=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #208=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #209=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #210=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #211=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #212=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #213=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #214=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #215=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #216=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #217=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #218=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #219=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #220=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #221=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #222=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #223=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #224=(CWidgetStyle "Enhancer Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #225=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #226=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #227=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 6723891) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #228=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 6723891) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #229=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 3368448) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #230=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #231=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 52479 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #232=(CWidgetStyle "Shape 24" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 3381759 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #233=(CWidgetStyle "Shape 25" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10066329 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #234=(CWidgetStyle "Shape 26" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 52275 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #235=(CWidgetStyle "Shape 27" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16763904 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #236=(CWidgetStyle "Shape 28" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13395456 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #237=(CWidgetStyle "Shape 29" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13408563 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #238=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 30" 1 "Attenuator Label") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 20") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 20") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 20") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Signal Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 9" 1 "Label 16") 68 
COMMENT          (CShapeMapEntry 0 "Shape 8" 1 "Label 23") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 44.1479 44.1479 39 39 0.1 
COMMENT         -11477) 1 1 0 1 1 
COMMENT        (mapper: 18.5907 -19.0446 44.1479 44.1479 0.01 10 -1 6141 6141 1 0 0) 
COMMENT        #239=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 761873453 100) 
COMMENT              (CObjectList 
COMMENT               #240=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -756 100) 
COMMENT                     (CObjectList 
COMMENT                      #241=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #148# 24704012 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27521 
COMMENT                             6.27321 1 0.0214037) 0.102153) 
COMMENT                      #242=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #142# 25136836 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 69 26 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pSV-Sport-di-RF-UNR(1-390)" "@N" 1 0 
COMMENT                            0.871165 0 -10 17.8264 1.56293 Nil) 
COMMENT                      #243=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #141# 2082602612 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 80 30 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "6141 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 5.14181 1.76679 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #244=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -887 100) 
COMMENT                     (CObjectList 
COMMENT                      #245=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -887 100) 
COMMENT                            (CObjectList 
COMMENT                             #246=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #144# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.91149 5.86764 1 0.082322) 0.8 1.8 0) 
COMMENT                             #247=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #208# 262166 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rLUC" "@N" 1 
COMMENT                                   10.3997 7.94513 0 -10 2.15186 1.13256 #246#) 
COMMENT                             #248=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #144# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.79387 4.48245 1 0.082322) 0.8 1.8 0) 
COMMENT                             #249=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "LUCm" "@N" 1 
COMMENT                                   7.10611 -10.9052 0 -10 2.65018 1.13256 #248#) 
COMMENT                             #250=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #144# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.303393 1.18293 1 0.082322) 0.8 1.8 1) 
COMMENT                             #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ampicillin" 
COMMENT                                   "@N" 1 -10.3462 9.45733 0 -10 3.91865 1.13256 
COMMENT                                   #250#)) (CObjectList)) 
COMMENT                      #252=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -465 100) 
COMMENT                            (CObjectList 
COMMENT                             #253=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #182# 595 100) 
COMMENT                                   (LOGPEN 0 0 3342336) 1 1 2.63145 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #254=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "intron SV40 small t-antigen" "@N" 1 -11.3927 
COMMENT                                   -10.4784 0 -10 11.2123 1.13256 #253#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #255=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1098 100) 
COMMENT                            (CObjectList 
COMMENT                             #256=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #202# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.98512 6.27319 1 0.082322) 0.8 1.8 0) 
COMMENT                             #257=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #208# 21590836 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 6.90415 16.8815 
COMMENT                                   0 -10 8.51683 1.13256 #256#)) (CObjectList)) 
COMMENT                      #258=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -1168 100) 
COMMENT                            (CObjectList 
COMMENT                             #259=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #5# 0) 1 2 0 0 #154# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 5.83451 5.92178) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #260=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #208# 14352944 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SP6 promoter" 
COMMENT                                   "@N" 1 8.08253 13.376 0 -10 5.75339 1.13256 
COMMENT                                   #259#) 
COMMENT                             #261=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #219# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    0.0449471 0.104196 1 0.082322) 0.8 1.8 0) 
COMMENT                             #262=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "lacUV5 promoter" "@N" 1 -4.47704 14.0972 0 
COMMENT                                   -10 6.9539 1.13256 #261#) 
COMMENT                             #263=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #154# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 2.73871 2.82598) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 -7.39032 -11.7415 0 -10 4.98325 
COMMENT                                   1.13256 #263#)) (CObjectList)) 
COMMENT                      #265=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -184 100) 
COMMENT                            (CObjectList 
COMMENT                             #266=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.954843 4.48449 
COMMENT                                   4.86655) 
COMMENT                             #267=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR (fragm. 1-390) of UNR (IRES)" "@N" 1 
COMMENT                                   21.3367 -0.94954 0 -10 14.8818 1.13256 #266#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #268=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -395 100) 
COMMENT                            (CObjectList 
COMMENT                             #269=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #143# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.72433 2.13601 1 0.082322) 0.8 1.8 1) 
COMMENT                             #270=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #208# 16777215 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -13.1829 -4.73228 0 -10 3.78274 1.13256 
COMMENT                                   #269#) 
COMMENT                             #271=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #143# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.835356 5.95243 6.03006 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #272=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 5.77482 14.6653 0 -10 3.62418 1.13256 #271#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #273=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -957 100) 
COMMENT                            (CObjectList 
COMMENT                             #274=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #152# 998 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 2.25042 
COMMENT                                   2.34951) 
COMMENT                             #275=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #208# 16777215 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 -13.2407 -8.80406 0 
COMMENT                                   -10 7.24836 1.13256 #274#) 
COMMENT                             #276=(CLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.670712 2.25042 
COMMENT                                   2.34951) 
COMMENT                             #277=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #208# 16777215 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 -13.3146 -7.4921 0 
COMMENT                                   -10 6.75004 1.13256 #276#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #278=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1309 100) 
COMMENT                     (CObjectList 
COMMENT                      #279=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -746 100) 
COMMENT                            (CObjectList 
COMMENT                             #280=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #199# 487 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 2.77957 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #281=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #198# 28090700 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Hin}dIII (3421)" "@N (@S)" 5 -7.91444 
COMMENT                                   -13.9631 0 -10 5.20976 1.0646 #280#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #282=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -676 100) 
COMMENT                            (CObjectList 
COMMENT                             #283=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #197# 514 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.87165 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #284=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 25397924 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Xba}I (1373)" "@N (@S)" 5 15.6337 
COMMENT                                   1.47232 0 -10 4.28106 1.0646 #283#) 
COMMENT                             #285=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #197# 514 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 2.79183 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #286=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 24791716 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Xba}I (3409)" "@N (@S)" 5 -7.63375 
COMMENT                                   -15.0694 0 -10 4.28106 1.0646 #285#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #287=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -1238 100) 
COMMENT                            (CObjectList 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.89004 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #289=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 24497500 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Ban}II (1355)" "@N (@S)" 5 15.7878 
COMMENT                                   2.73898 0 -10 4.55288 1.0646 #288#) 
COMMENT                             #290=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #197# 761873453 
COMMENT                                    100) (LOGPEN 0 0 3381504) 1 1 3.43539 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #291=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 24934876 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Ban}II (2779)" "@N (@S)" 5 6.18154 
COMMENT                                   -12.6022 0 -10 4.55288 1.0646 #290#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #292=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #1# 0) 1 2 0 0 Nil -1379 100) 
COMMENT                            (CObjectList 
COMMENT                             #293=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.03721 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #294=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 25745188 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Nco}I (232)" "@N (@S)" 5 5.67807 
COMMENT                                   15.7054 0 -10 3.85069 1.0646 #293#) 
COMMENT                             #295=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.86866 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #296=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 25062724 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Nco}I (397)" "@N (@S)" 5 7.22852 
COMMENT                                   12.1773 0 -10 3.85069 1.0646 #295#) 
COMMENT                             #297=(CScratch 
COMMENT                                   (CWidget 3 (1 #1# 3) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.48347 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #298=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 24935140 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Nco}I (1753)" "@N (@S)" 5 15.5238 
COMMENT                                   -4.32094 0 -10 4.28106 1.0646 #297#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #299=(CGroupWidget 
COMMENT                            (CWidget 5 (10 #4# 0) 1 2 0 0 Nil -959 100) 
COMMENT                            (CObjectList 
COMMENT                             #300=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.6807 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #301=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 21205024 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Afl}III (581)" "@N (@S)" 5 8.71223 
COMMENT                                   10.1405 0 -10 4.00925 1.0646 #300#) 
COMMENT                             #302=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.49267 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #303=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 25422432 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Afl}III (1744)" "@N (@S)" 5 15.7643 
COMMENT                                   -3.2355 0 -10 4.43962 1.0646 #302#) 
COMMENT                             #304=(CScratch 
COMMENT                                   (CWidget 3 (1 #4# 3) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 3.97782 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #305=(CLabel (CWidget 0 (0 0) 1 2 0 0 #212# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Afl}III (2248)" "@N (@S)" 5 11.25 
COMMENT                                   -8.55309 0 -10 4.43962 1.0646 #304#) 
COMMENT                             #306=(CScratch 
COMMENT                                   (CWidget 4 (1 #4# 4) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 2.76935 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #307=(CLabel (CWidget 0 (0 0) 1 2 0 0 #212# 16 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Afl}III (3431)" "@N (@S)" 5 -7.26711 
COMMENT                                   -12.9456 0 -10 4.43962 1.0646 #306#) 
COMMENT                             #308=(CScratch 
COMMENT                                   (CWidget 5 (1 #4# 5) 1 2 0 0 #197# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 2.00934 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #309=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #212# 1699160122 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Afl}III (4175)" "@N (@S)" 5 -13.477 
COMMENT                                   -5.84038 0 -10 4.43962 1.0646 #308#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #310=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1030 100) 
COMMENT                     (CObjectList 
COMMENT                      #311=(CLabel 
COMMENT                            (CWidget 0 (15 #18# 0) 1 2 0 0 #132# 1701470831 100) 
COMMENT                            (LOGPEN 1734963791 1818324585 980578152) 1918987552 
COMMENT                            (LOGFONT 69 26 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 5258" 
COMMENT                            "LMBP 5258" 65535 0 -2.55958 0 -10 6.9992 1.56293 
COMMENT                            Nil) 
COMMENT                      #312=(CLabel 
COMMENT                            (CWidget 0 (15 #19# 0) 1 2 0 0 #132# 25958756 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 29-OCT-2007" 
COMMENT                            "Last modified: 29-OCT-2007" 65535 -0.339767 -3.896 
COMMENT                            0 -10 11.1444 1.0646 Nil)) (CObjectList)) 
COMMENT               #313=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -257 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 6 6 (CObList)))
FEATURES             Location/Qualifiers
     promoter        345..361
                     /vntifkey="30"
                     /label=SP6\promoter
     promoter        1..282
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             398..1333
                     /vntifkey="4"
                     /label=rLUC
     CDS             1754..3406
                     /vntifkey="4"
                     /label=LUCm
                     /note="mutated firefly luciferase coding sequence"
     promoter        6040..6097
                     /vntifkey="30"
                     /label=lacUV5\promoter
     promoter        complement(3461..3477)
                     /vntifkey="30"
                     /label=T7\promoter
     intron          3566..3605
                     /vntifkey="15"
                     /label=intron\SV40\small\t-antigen
                     /note="intron of the SV40 small t-antigen"
     terminator      3842..3938
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(3842..3938)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(4051..4453)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(4984..5844)
                     /vntifkey="4"
                     /label=ampicillin
     RBS             1378..1751
                     /vntifkey="32"
                     /label=5'\UTR\(fragm.\1-390)\of\UNR\(IRES)
     rep_origin      239..314
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
BASE COUNT     1685 a      1366 c      1420 g      1670 t 
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta 
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat 
      361 agaaggtacg cctgcaggta ccggtccgga attcgccatg gcttcgaaag tttatgatcc 
      421 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa 
      481 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat 
      541 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga 
      601 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg 
      661 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct 
      721 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca 
      781 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga 
      841 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc 
      901 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc 
      961 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga 
     1021 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg 
     1081 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga 
     1141 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga 
     1201 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca 
     1261 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa 
     1321 aaatgaacaa taagaattcc cgggtcgacg agctcactag tcggcggccg ctctagatgc 
     1381 tgcttatggc ggcgctggag agggggcgct gagctgttgg gtatgaagtg taacagaaca 
     1441 gactttacca cctgaaactg ctgcttcaag ttcagatcag gcaaggaaca aacctcgtaa 
     1501 caactaacaa gaccaaagaa gagtacactt aagttgaaga cacaacactt gatctgaaac 
     1561 aagaagtttg tgcctactca acagctttga aagagcactt cccaacgctg ctagtagtct 
     1621 ttgttttctt cagtgctgta ctgtgagatt gcccggtaca gcagcagttg tattctttat 
     1681 tagcttggta gatcattttc tctcgctctt ttttttaata ctagcaactt tcatcctttg 
     1741 aaacgtgtgc tccatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc 
     1801 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt 
     1861 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta 
     1921 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca 
     1981 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt 
     2041 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa 
     2101 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat 
     2161 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac 
     2221 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt 
     2281 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat 
     2341 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg 
     2401 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc 
     2461 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt 
     2521 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag 
     2581 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc 
     2641 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg 
     2701 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat 
     2761 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga 
     2821 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct 
     2881 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat 
     2941 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg 
     3001 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga 
     3061 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc 
     3121 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga 
     3181 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa 
     3241 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt 
     3301 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag 
     3361 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agaggatcca 
     3421 agcttacgta cgcgtgcatg cgacgtcata gctctctccc tatagtgagt cgtattataa 
     3481 gctagcttgg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac 
     3541 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta 
     3601 aactagctgc atatgcttgc tgcttgagag ttttgcttac tgagtatgat ttatgaaaat 
     3661 attatacaca ggagctagtg attctaattg tttgtgtatt ttagattcac agtcccaagg 
     3721 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat 
     3781 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata 
     3841 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa 
     3901 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     3961 tgtccaaact catcaatgta tcttatcatg tctggatcct gcattaatga atcggccaac 
     4021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 
     4081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 
     4141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     4201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     4261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     4321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     4381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct 
     4441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     4501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     4561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     4621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 
     4681 tatttggtat ctgcggccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     4741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     4801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     4861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     4921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     4981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     5041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     5101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     5161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     5221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     5281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     5341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     5401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     5461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     5521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     5581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     5641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     5701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     5761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     5821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     5881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     5941 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 
     6001 gacattaacc tataaaaata ggcgtatacg aggccctttc actcattagg caccccaggc 
     6061 tttacacttt atagcttccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc 
     6121 acacaggaaa cagcatcgat g 
//
