LOCUS       pSV-Sport-Raf-1E        5246 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 5264.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5264
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
COMMENT     VNTDATE|456504519|
COMMENT     VNTNAME|pSV-Sport-Raf-1E|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSV-Sport-Raf-1E" 0 5246 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "AvaII" "EcoRI" "HaeII" "NotI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1 5246 1 5246 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 390 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2341 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2514 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 384 0 1363 0 4301 0 4523 
COMMENT            0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 4 515 0 2254 0 3158 0 3528 
COMMENT            0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "SP6 promoter" 30 0 0 345 361 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "345..361") 
COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 0 239 314 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "239..314") 
COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 0 1 282 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1..282") 
COMMENT        #8=(CFSignal (CObList) "ampicillin" 4 0 1 4089 4949 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2169..3029)") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 3156 3558 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1236..1638)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2947 3043 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1027..1123)") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2947 3043 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1027..1123") 
COMMENT        #12=(CFSignal (CObList) "intron of SV40 small t-antigen" 15 0 0 2671 
COMMENT             2710 0 (CStringList) (CStringList) 1 1 1 1 "751..790") 
COMMENT        #13=(CFSignal (CObList) "T7 promoter" 30 0 1 2566 2582 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(646..662)") 
COMMENT        #14=(CFSignal (CObList) "E-tag" 22 0 0 2348 2386 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "428..466") 
COMMENT        #15=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 5145 5202 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5144..5202") 
COMMENT        #16=(CFSignal (CObList) "hRaf1" 4 0 0 395 2338 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "395..2338")) (CObList) (CObList) (CObList) 
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COMMENT        #17=(CFSignal (CObList) "LMBP 5264" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pSV-Sport-Raf-1E'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5264." 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: margotbaetens" 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/03/07 02:48" 1) 
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COMMENT                          "Last Modified: 10/29/07 04:00" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5246 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 7-FEB-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "EMAIL: <a href=\"mailto:margot.baetens@dmbr.ugent.be\">margot.baetens@dmbr.ugent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "" 1))) "LMBP 5264") 
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COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                                        "   751..790" 1))) 
COMMENT                                 "intron of SV40 small t-antigen"))) 
COMMENT                          "Intron (1 total)") 
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COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                   #71=(CLinePar 
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COMMENT                                        "Start: 2566  End: 2582 (Complementary)" 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                   #75=(CLinePar 
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COMMENT                                        "   complement(646..662)" 1))) 
COMMENT                                 "T7 promoter") 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                        "mutated E. coli lac operon promoter (lacUV5 promoter)" 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                   #80=(CLinePar 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                        "   239..314" 1))) "SV40 ori") 
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COMMENT                                        "   complement(1236..1638)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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FEATURES             Location/Qualifiers
     promoter        345..361
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="phage SP6 promoter"
     rep_origin      239..314
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        1..282
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /note="SV40 early promoter"
     promoter        5145..5202
                     /vntifkey="30"
                     /label=lacUV5\promoter
                     /note="mutated E. coli lac operon promoter (lacUV5 promoter)"
     CDS             complement(4089..4949)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(3156..3558)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(2947..3043)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late  region"
     terminator      2947..3043
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     intron          2671..2710
                     /vntifkey="15"
                     /label=intron\of\SV40\small\t-antigen
                     /note="intron of the SV40 small t-antigen"
     promoter        complement(2566..2582)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     misc_marker     2348..2386
                     /vntifkey="22"
                     /label=E-tag
                     /note="C-terminal E-tag"
     CDS             395..2338
                     /vntifkey="4"
                     /label=hRaf1
BASE COUNT     1391 a      1291 c      1218 g      1346 t 
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta 
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat 
      361 agaaggtacg cctgcaggta ccggtccgga attcatggag cacatacagg gagcttggaa 
      421 gacgatcagc aatggttttg gattcaaaga tgccgtgttt gatggctcca gctgcatctc 
      481 tcctacaata gttcagcagt ttggctatca gcgccgggca tcagatgatg gcaaactcac 
      541 agatccttct aagacaagca acactatccg tgttttcttg ccgaacaagc aaagaacagt 
      601 ggtcaatgtg cgaaatggaa tgagcttgca tgactgcctt atgaaagcac tcaaggtgag 
      661 gggcctgcaa ccagagtgct gtgcagtgtt cagacttctc cacgaacaca aaggtaaaaa 
      721 agcacgctta gattggaata ctgatgctgc gtctttgatt ggagaagaac ttcaagtaga 
      781 tttcctggat catgttcccc tcacaacaca caactttgct cggaagacgt tcctgaagct 
      841 tgccttctgt gacatctgtc agaaattcct gctcaatgga tttcgatgtc agacttgtgg 
      901 ctacaaattt catgagcact gtagcaccaa agtacctact atgtgtgtgg actggagtaa 
      961 catcagacaa ctcttattgt ttccaaattc cactattggt gatagtggag tcccagcact 
     1021 accttctttg actatgcgtc gtatgcgaga gtctgtttcc aggatgcctg ttagttctca 
     1081 gcacagatat tctacacctc acgccttcac ctttaacacc tccagtccct catctgaagg 
     1141 ttccctctcc cagaggcaga ggtcgacatc cacacctaat gtccacatgg tcagcaccac 
     1201 cctgcctgtg gacagcagga tgattgagga tgcaattcga agtcacagcg aatcagcctc 
     1261 accttcagcc ctgtccagta gccccaacaa tctgagccca acaggctggt cacagccgaa 
     1321 aacccccgtg ccagcacaaa gagagcgggc accagtatct gggacccagg agaaaaacaa 
     1381 aattaggcct cgtggacaga gagattcaag ctattattgg gaaatagaag ccagtgaagt 
     1441 gatgctgtcc actcggattg ggtcaggctc ttttggaact gtttataagg gtaaatggca 
     1501 cggagatgtt gcagtaaaga tcctaaaggt tgtcgaccca accccagagc aattccaggc 
     1561 cttcaggaat gaggtggctg ttctgcgcaa aacacggcat gtgaacattc tgcttttcat 
     1621 ggggtacatg acaaaggaca acctggcaat tgtgacccag tggtgcgagg gcagcagcct 
     1681 ctacaaacac ctgcatgtcc aggagaccaa gtttcagatg ttccagctaa ttgacattgc 
     1741 ccggcagacg gctcagggaa tggactattt gcatgcaaag aacatcatcc atagagacat 
     1801 gaaatccaac aatatatttc tccatgaagg cttaacagtg aaaattggag attttggttt 
     1861 ggcaacagta aagtcacgct ggagtggttc tcagcaggtt gaacaaccta ctggctctgt 
     1921 cctctggatg gccccagagg tgatccgaat gcaggataac aacccattca gtttccagtc 
     1981 ggatgtctac tcctatggca tcgtattgta tgaactgatg acgggggagc ttccttattc 
     2041 tcacatcaac aaccgagatc agatcatctt catggtgggc cgaggatatg cctccccaga 
     2101 tcttagtaag ctatataaga actgccccaa agcaatgaag aggctggtag ctgactgtgt 
     2161 gaagaaagta aaggaagaga ggcctctttt tccccagatc ctgtcttcca ttgagctgct 
     2221 ccaacactct ctaccgaaga tcaaccggag cgcttccgag ccatccttgc atcgggcagc 
     2281 ccacactgag gatatcaatg cttgcacgct gaccacgtcc ccgaggctgc ctgtcttcgc 
     2341 ggccgcaggt gcgccggtgc cgtatccgga tccgctggaa ccgcgtgccg catagactgt 
     2401 tgaaagttgt ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga 
     2461 caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgtcg actctagagg 
     2521 atccaagctt acgtacgcgt gcatgcgacg tcatagctct ctccctatag tgagtcgtat 
     2581 tataagctag cttgggatct ttgtgaagga accttacttc tgtggtgtga cataattgga 
     2641 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg 
     2701 tgttaaacta gctgcatatg cttgctgctt gagagttttg cttactgagt atgatttatg 
     2761 aaaatattat acacaggagc tagtgattct aattgtttgt gtattttaga ttcacagtcc 
     2821 caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac 
     2881 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 
     2941 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 
     3001 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     3061 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctgcatt aatgaatcgg 
     3121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     3181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     3241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     3301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     3361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     3421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     3481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 
     3541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     3601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     3661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     3721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     3781 gacagtattt ggtatctgcg gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     3841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     3901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     3961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     4021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     4081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     4141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     4201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     4261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     4321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     4381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     4441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     4501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     4561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     4621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     4681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     4741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     4801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     4861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     4921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     4981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     5041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 
     5101 atcatgacat taacctataa aaataggcgt atacgaggcc ctttcactca ttaggcaccc 
     5161 caggctttac actttatagc ttccggctcg tataatgtgt ggaattgtga gcggataaca 
     5221 atttcacaca ggaaacagca tcgatg 
//
