LOCUS       pEF-BOS-HA-IRF3Gal4      4859 bp    DNA     circular UNA 
DEFINITION  LMBP 5275.
FEATURES             Location/Qualifiers
     terminator      complement(130..226)
                     /label="SV40 polyA late"
     terminator      130..226
                     /label="SV40 polyA early"
     promoter        complement(299..315)
                     /label="T7 promoter"
     rep_origin      complement(389..791)
                     /label="pMB1 ori"
     CDS             complement(1332..2192)
                     /label="ampicillin resistance"
     promoter        2643..2659
                     /label="SP6 promoter"
     promoter        2672..2945
                     /label="SV40 early promoter"
     rep_origin      2902..2977
                     /label="SV40 ori"
     CDS             3018..3458
                     /label="GALbd"
     CDS             3471..4754
                     /label="IRF3"
ORIGIN
        1 CTCGAGACTA GTGCGGCCGC TCGAGCATGC ATCTAGATAA GTAATGATCA TAATCAGCCA
       61 TATCACATCT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT
      121 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA
      181 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
      241 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGCC GGTCTCCCTA
      301 TAGTGAGTCG TATTAATTTC GATAAGCCAG GTTAACCTGC ATTAATGAAT CGGCCAACGC
      361 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
      421 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
      481 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
      541 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG
      601 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
      661 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
      721 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
      781 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
      841 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
      901 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
      961 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA
     1021 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
     1081 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
     1141 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1201 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1261 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1321 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1381 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     1441 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCAGC TCCAGATTTA
     1501 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     1561 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     1621 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     1681 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     1741 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     1801 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     1861 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     1921 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     1981 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2041 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2101 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2161 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT
     2341 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT
     2401 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT
     2461 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG
     2521 TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG
     2581 GACATATTGT CGTTAGAACG CGGCTACAAT TAATACATAA CCTTATGTAT CATACACATA
     2641 CGATTTAGGT GACACTATAG AACTCGAGCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG
     2701 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
     2761 AAGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT
     2821 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG
     2881 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT
     2941 CGGCCTCTGA GCTATTCCAG AAGTAGTGAA GAGGCTTTTT TGGAGGAGAT CTAAGCTTGA
     3001 AGCAAGCCTC CTGAAAGATG AAGCTACTGT CTTCTATCGA ACAAGCATGC GATATTTGCC
     3061 GACTTAAAAA GCTCAAGTGC TCCAAAGAAA AACCGAAGTG CGCCAAGTGT CTGAAGAACA
     3121 ACTGGGAGTG TCGCTACTCT CCCAAAACCA AAAGGTCTCC GCTGACTAGG GCACATCTGA
     3181 CAGAAGTGGA ATCAAGGCTA GAAAGACTGG AACAGCTATT TCTACTGATT TTTCCTCGAG
     3241 AAGACCTTGA CATGATTTTG AAAATGGATT CTTTACAGGA TATAAAAGCA TTGTTAACAG
     3301 GATTATTTGT ACAAGATAAT GTGAATAAAG ATGCCGTCAC AGATAGATTG GCTTCAGTGG
     3361 AGACTGATAT GCCTCTAACA TTGAGACAGC ATAGAATAAG TGCGACATCA TCATCGGAAG
     3421 AGAGTAGTAA CAAAGGTCAA AGACAGTTGA CTGTATCGCC GGAATTCACC ATGGGAACCC
     3481 CAAAGCCACG GATCCTGCCC TGGCTGGTGT CGCAGCTGGA CCTGGGGCAA CTGGAGGGCG
     3541 TGGCCTGGGT GAACAAGAGC CGCACGCGCT TCCGCATCCC TTGGAAGCAC GGCCTACGGC
     3601 AGGATGCACA GCAGGAGGAT TTCGGAATCT TCCAGGCCTG GGCCGAGGCC ACTGGTGCAT
     3661 ATGTTCCCGG GAGGGATAAG CCAGACCTGC CAACCTGGAA GAGGAATTTC CGCTCTGCCC
     3721 TCAACCGCAA AGAAGGGTTG CGTTTAGCAG AGGACCGGAG CAAGGACCCT CACGACCCAC
     3781 ATAAAATCTA CGAGTTTGTG AACTCAGGAG TTGGGGACTT TTCCCAGCCA GACACCTCTC
     3841 CGGACACCAA TGGTGGAGGC AGTACTTCTG ATACCCAGGA AGACATTCTG GATGAGTTAC
     3901 TGGGTAACAT GGTGTTGGCC CCACTCCCAG ATCCGGGACC CCCAAGCCTG GCTGTAGCCC
     3961 CTGAGCCCTG CCCTCAGCCC CTGCGGAGCC CCAGCTTGGA CAATCCCACT CCCTTCCCAA
     4021 ACCTGGGGCC CTCTGAGAAC CCACTGAAGC GGCTGTTGGT GCCGGGGGAA GAGTGGGAGT
     4081 TCGAGGTGAC AGCCTTCTAC CGGGGCCGCC AAGTCTTCCA GCAGACCATC TCCTGCCCGG
     4141 AGGGCCTGCG GCTGGTGGGG TCCGAAGTGG GAGACAGGAC GCTGCCTGGA TGGCCAGTCA
     4201 CACTGCCAGA CCCTGGCATG TCCCTGACAG ACAGGGGAGT GATGAGCTAC GTGAGGCATG
     4261 TGCTGAGCTG CCTGGGTGGG GGACTGGCTC TCTGGCGGGC CGGGCAGTGG CTCTGGGCCC
     4321 AGCGGCTGGG GCACTGCCAC ACATACTGGG CAGTGAGCGA GGAGCTGCTC CCCAACAGCG
     4381 GGCATGGGCC TGATGGCGAG GTCCCCAAGG ACAAGGAAGG AGGCGTGTTT GACCTGGGGC
     4441 CCTTCATTGT AGATCTGATT ACCTTCACGG AAGGAAGCGG ACGCTCACCA CGCTATGCCC
     4501 TCTGGTTCTG TGTGGGGGAG TCATGGCCCC AGGACCAGCC GTGGACCAAG AGGCTCGTGA
     4561 TGGTCAAGGT TGTGCCCACG TGCCTCAGGG CCTTGGTAGA AATGGCCCGG GTAGGGGGTG
     4621 CCTCCTCCCT GGAGAATACT GTGGACCTGC ACATTTCCAA CAGCCACCCA CTCTCCCTCA
     4681 CCTCCGACCA GTACAAGGCC TACCTGCAGG ACTTGGTGGA GGGCATGGAT TTCCAGGGCC
     4741 CTGGGGAGAG CTGAGCCCTC GCTCCTCATG GTGTGCCTCC AACCCCCCTG TTCCCCACCA
     4801 CCTCAACCAA TAAACTGGTT CCTGCTATGA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA
//
