LOCUS       pEF1hCASP-9-His         7393 bp    DNA     circular     23-OCT-2008
DEFINITION  LMBP 5287.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5287
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-OCT-2008
            
COMMENT     VNTDATE|496942654|
COMMENT     VNTNAME|pEF1hCASP-9-His|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1hCASP-9-His" 0 7393 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AatII" "EcoRI" "NcoI" "SalI" "SspI" "XbaI" "AdeI" "CaiI" 
COMMENT         "PdmI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 7116 7115 1 7393 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1749 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 122 0 1841 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 1757 0 2637 0 4055 0 4790 
COMMENT            0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 121 0 5337 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SspI" "aatatt" 4 0 1 4 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3007 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AdeI" "cacnnngtg" 7 0 3 1510 0 2773 0 3625 0 0 
COMMENT            "") 
COMMENT        #7=(CRSite (CStringList) "CaiI" "cagnnnctg" 7 0 4 203 0 1900 0 2503 0 
COMMENT            6116 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 4 773 0 2554 0 3820 0 
COMMENT            7192 0 0 "")) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "hEF1 promoter" 29 0 0 508 1651 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "508..1651") 
COMMENT        #10=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1668..1684") 
COMMENT        #11=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 704 1642 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "704..1642") 
COMMENT        #12=(CFSignal (CObList) "hCASP-9" 4 0 0 1758 3005 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1758..3005") 
COMMENT        #13=(CFSignal (CObList) "V5 epitope" 22 0 0 3024 3065 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3024..3065") 
COMMENT        #14=(CFSignal (CObList) "His-tag" 22 0 0 3075 3092 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3075..3092") 
COMMENT        #15=(CFSignal (CObList) "BGH polyA" 43 0 0 3121 3345 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3121..3345") 
COMMENT        #16=(CFSignal (CObList) "f1 ori" 33 0 0 3391 3819 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3391..3819") 
COMMENT        #17=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3824 4105 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3824..4105") 
COMMENT        #18=(CFSignal (CObList) "SV40 ori" 33 0 0 4062 4137 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4062..4137") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5199 5273 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5199..5273") 
COMMENT        #20=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5199 5273 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5199..5273)") 
COMMENT        #21=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6519 7379 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6519..7379)") 
COMMENT        #22=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4229 5023 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4229..5023") 
COMMENT        #23=(CFSignal (CObList) "pMB1 ori" 33 0 1 5587 5978 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5587..5978)") 
COMMENT        #24=(CFSignal (CObList) "lac promoter" 30 0 1 5412 5495 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #25=(CFSignal (CObList) "LMBP 5287" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "Last modified: 23-OCT-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #27=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #28=(CRefLinePar 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pEF1hCASP-9-His'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5287" 
COMMENT                          1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 10/23/08 03:37" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/23/08 03:39" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7393 bp" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #40=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #41=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5287") 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CLinePar (CParagraph 0 (15 #26# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 23-OCT-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #46=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1758 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1758  End: 3005" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1758..3005" 1))) "hCASP-9") 
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COMMENT                                  (CParagraph 4229 (3 #22# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4229  End: 5023" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4229..5023" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
COMMENT                            #56=(CFolderPar 
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COMMENT                                  (CParagraph 6519 (3 #21# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6519  End: 7379 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6519..7379)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
COMMENT                     #60=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 704   End: 1642" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   704..1642" 1))) 
COMMENT                                 "intron hEF1 promoter"))) "Intron (1 total)") 
COMMENT                     #65=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3024  End: 3065" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3024..3065" 1))) "V5 epitope") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3075 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3075  End: 3092" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3075..3092" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #74=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 508 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 508   End: 1651" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   508..1651" 1))) "hEF1 promoter") 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3824 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3824  End: 4105" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3824..4105" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1668 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1668  End: 1684" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1668..1684" 1))) "T7 promoter") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5412 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5412  End: 5495 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3391 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3391  End: 3819" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3391..3819" 1))) "f1 ori") 
COMMENT                            #95=(CFolderPar 
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COMMENT                                  (CParagraph 4062 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4062  End: 4137" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4062..4137" 1))) "SV40 ori") 
COMMENT                            #99=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 5587  End: 5978 (Complementary)" 
COMMENT                                         1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(5587..5978)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #103=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                     #106=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3121..3345" 1))) "BGH polyA") 
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COMMENT                                    (CObjectList 
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COMMENT                                     #110=(CLinePar 
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COMMENT                                     #111=(CLinePar 
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COMMENT                                   "SV40 polyA early") 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 5199  End: 5273 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #114=(CLinePar 
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COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(5199..5273)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #116=(CFolderPar 
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COMMENT                     (CObjectList 
COMMENT                      #117=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 1749 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #120=(CRSFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4 " 1))))) "SspI: 1 site") 3 3 
COMMENT                            "AATATT" "TTATAA") 
COMMENT                      #123=(CRSFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3007 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 122 " 1) 
COMMENT                                      #129=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1841 " 1))))) "AatII: 2 sites") 
COMMENT                            5 1 "GACGTC" "CTGCAG") 
COMMENT                      #130=(CRSFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 121 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5337 " 1))))) "SalI: 2 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #134=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #136=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1510 " 1) 
COMMENT                                      #137=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2773 " 1) 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3625 " 1))))) "AdeI: 3 sites") 
COMMENT                            6 3 "CACNNNGTG" "GTGNNNCAC") 
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FEATURES             Location/Qualifiers
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     intron          704..1642
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     CDS             1758..3005
                     /vntifkey="4"
                     /label=hCASP-9
     misc_marker     3024..3065
                     /vntifkey="22"
                     /label=V5\epitope
     misc_marker     3075..3092
                     /vntifkey="22"
                     /label=His-tag
     terminator      3121..3345
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3391..3819
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3824..4105
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4062..4137
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      5199..5273
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5199..5273)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(6519..7379)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             4229..5023
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      complement(5587..5978)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(5412..5495)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1580 a      1939 c      2036 g      1838 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 
     1741 tgtggtggaa ttccgccatg gacgaagcgg atcggcggct cctgcggcgg tgccggctgc 
     1801 ggctggtgga agagctgcag gtggaccagc tctgggacgt cctgctgagc cgcgagctgt 
     1861 tcaggcccca tatgatcgag gacatccagc gggcaggctc tggatctcgg cgggatcagg 
     1921 ccaggcagct gatcatagat ctggagactc gagggagtca ggctcttcct ttgttcatct 
     1981 cctgcttaga ggacacaggc caggacatgc tggcttcgtt tctgcgaact aacaggcaag 
     2041 caggaaagtt gtcgaagcca accctagaaa accttacccc agtggtgctc agaccagaga 
     2101 ttcgcaaacc agaggttctc agaccggaaa cacccagacc agtggacatt ggttctggag 
     2161 gattcggtga tgtcggtgct cttgagagtt tgaggggaaa tgcagatttg gcttacatcc 
     2221 tgagcatgga gccctgtggc cactgcctca ttatcaacaa tgtgaacttc tgccgtgagt 
     2281 ccgggctccg cacccgcact ggctccaaca tcgactgtga gaagttgcgg cgtcgcttct 
     2341 cctcgctgca tttcatggtg gaggtgaagg gcgacctgac tgccaagaaa atggtgctgg 
     2401 ctttgctgga gctggcgcgg caggaccacg gtgctctgga ctgctgcgtg gtggtcattc 
     2461 tctctcacgg ctgtcaggcc agccacctgc agttcccagg ggctgtctac ggcacagatg 
     2521 gatgccctgt gtcggtcgag aagattgtga acatcttcaa tgggaccagc tgccccagcc 
     2581 tgggagggaa gcccaagctc tttttcatcc aggcctgtgg tggggagcag aaagaccatg 
     2641 ggtttgaggt ggcctccact tcccctgaag acgagtcccc tggcagtaac cccgagccag 
     2701 atgccacccc gttccaggaa ggtttgagga ccttcgacca gctggacgcc atatctagtt 
     2761 tgcccacacc cagtgacatc tttgtgtcct actctacttt cccaggtttt gtttcctgga 
     2821 gggaccccaa gagtggctcc tggtacgttg agaccctgga cgacatcttt gagcagtggg 
     2881 ctcactctga agacctgcag tccctcctgc ttagggtcgc taatgctgtt tcggtgaaag 
     2941 ggatttataa acagatgcct ggttgcttta atttcctccg gaaaaaactt ttctttaaaa 
     3001 catcatctag agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt 
     3061 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga 
     3121 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 
     3181 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 
     3241 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 
     3301 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 
     3361 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 
     3421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     3481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     3541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     3601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     3661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     3721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     3781 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 
     3841 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     3901 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     3961 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 
     4021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     4081 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 
     4141 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 
     4201 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     4261 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     4321 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     4381 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     4441 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     4501 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 
     4561 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     4621 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     4681 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     4741 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     4801 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     4861 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     4921 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     4981 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 
     5041 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 
     5101 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 
     5161 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 
     5341 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     5401 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     5461 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     5521 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     5581 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     5641 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     5701 caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     5761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     5821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     5881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     5941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     6001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     6061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     6121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     6181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     6241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     6301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     6361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     6421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     6481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     6541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     6601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     6661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     6721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     6781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     6841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     6901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     6961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     7021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     7081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     7141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     7201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     7261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     7321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     7381 ctcttccttt ttc 
//
