ID pEF1hCASP-9-His standard; circular DNA; ; 7393 BP. DE LMBP 5287. CC Accession number: LMBP 5287 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 23-OCT-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT intron 704..1642 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT CDS 1758..3005 FT /vntifkey="4" FT /label=hCASP-9 FT misc_marker 3024..3065 FT /vntifkey="22" FT /label=V5\epitope FT misc_marker 3075..3092 FT /vntifkey="22" FT /label=His-tag FT terminator 3121..3345 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 3391..3819 FT /vntifkey="33" FT /label=f1\ori FT promoter 3824..4105 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4062..4137 FT /vntifkey="33" FT /label=SV40\ori FT terminator 5199..5273 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(5199..5273) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS complement(6519..7379) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS 4229..5023 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin complement(5587..5978) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(5412..5495) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 7393 BP; 1580 A; 1939 C; 2036 G; 1838 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 1740 tgtggtggaa ttccgccatg gacgaagcgg atcggcggct cctgcggcgg tgccggctgc 1800 ggctggtgga agagctgcag gtggaccagc tctgggacgt cctgctgagc cgcgagctgt 1860 tcaggcccca tatgatcgag gacatccagc gggcaggctc tggatctcgg cgggatcagg 1920 ccaggcagct gatcatagat ctggagactc gagggagtca ggctcttcct ttgttcatct 1980 cctgcttaga ggacacaggc caggacatgc tggcttcgtt tctgcgaact aacaggcaag 2040 caggaaagtt gtcgaagcca accctagaaa accttacccc agtggtgctc agaccagaga 2100 ttcgcaaacc agaggttctc agaccggaaa cacccagacc agtggacatt ggttctggag 2160 gattcggtga tgtcggtgct cttgagagtt tgaggggaaa tgcagatttg gcttacatcc 2220 tgagcatgga gccctgtggc cactgcctca ttatcaacaa tgtgaacttc tgccgtgagt 2280 ccgggctccg cacccgcact ggctccaaca tcgactgtga gaagttgcgg cgtcgcttct 2340 cctcgctgca tttcatggtg gaggtgaagg gcgacctgac tgccaagaaa atggtgctgg 2400 ctttgctgga gctggcgcgg caggaccacg gtgctctgga ctgctgcgtg gtggtcattc 2460 tctctcacgg ctgtcaggcc agccacctgc agttcccagg ggctgtctac ggcacagatg 2520 gatgccctgt gtcggtcgag aagattgtga acatcttcaa tgggaccagc tgccccagcc 2580 tgggagggaa gcccaagctc tttttcatcc aggcctgtgg tggggagcag aaagaccatg 2640 ggtttgaggt ggcctccact tcccctgaag acgagtcccc tggcagtaac cccgagccag 2700 atgccacccc gttccaggaa ggtttgagga ccttcgacca gctggacgcc atatctagtt 2760 tgcccacacc cagtgacatc tttgtgtcct actctacttt cccaggtttt gtttcctgga 2820 gggaccccaa gagtggctcc tggtacgttg agaccctgga cgacatcttt gagcagtggg 2880 ctcactctga agacctgcag tccctcctgc ttagggtcgc taatgctgtt tcggtgaaag 2940 ggatttataa acagatgcct ggttgcttta atttcctccg gaaaaaactt ttctttaaaa 3000 catcatctag agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt 3060 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga 3120 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 3180 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 3240 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 3300 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 3360 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 3420 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 3480 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 3540 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 3600 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 3660 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 3720 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 3780 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 3840 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3900 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3960 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 4020 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 4080 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 4140 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 4200 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 4260 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 4320 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 4380 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 4440 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 4500 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 4560 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 4620 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 4680 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 4740 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 4800 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 4860 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 4920 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 4980 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 5040 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 5100 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 5160 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 5220 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5280 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 5340 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5400 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5460 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5520 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 5580 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 5640 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 5700 caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5760 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5820 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5880 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 5940 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6000 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6060 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6120 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6180 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 6240 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6300 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6360 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6420 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6480 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6540 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6600 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6660 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6720 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6780 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6840 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6900 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6960 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7020 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7080 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 7140 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 7200 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7260 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7320 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7380 ctcttccttt ttc 7393 //