LOCUS       pLT32hC14wt             3696 bp    DNA     circular     13-FEB-2006
DEFINITION  LMBP 5337.
SOURCE      
  ORGANISM  
COMMENT     DMB 5337
COMMENT     VNTDATE|402082947|
COMMENT     VNTNAME|pLT32hC14wt|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT             (CTerm 2 0 213 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 103 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pLHX32hC14" "" 0 0 1 
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COMMENT             (CTerm 2 1 732 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
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COMMENT        #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 3051 0 3189 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 3183 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 8 0 2863 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 1711 0 3693 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 3053 0 3191 0 0 "") 
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COMMENT        #18=(CFSignal (CObList) "hC14" 4 0 0 2968 3696 1 (CStringList) 
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COMMENT                          "DNA 'pLT32hC14wt'" 1) 
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COMMENT                          "Currently local object. Original author: kkersse" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/13/06 05:42" 1) 
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COMMENT                          "Last Modified: 02/13/06 05:42" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3696 bp" 1) 
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FEATURES             Location/Qualifiers
     misc_difference 2755..2755
                     /vntifkey="85"
                     /label=+1
     promoter        2649..2760
                     /vntifkey="30"
                     /label=pL
     -10_signal      2737..2742
                     /vntifkey="47"
                     /label=-10
     -35_signal      2714..2719
                     /vntifkey="48"
                     /label=-35
     rep_origin      complement(710..910)
                     /vntifkey="33"
                     /label=ori
     CDS             complement(1392..2252)
                     /vntifkey="4"
                     /label=bla
     CDS             2968..3696
                     /vntifkey="4"
                     /label=hC14
BASE COUNT     1009 a       893 c       895 g       899 t 
ORIGIN
        1 gtcgacgaat tcgagctcgg ccgacttggc cttcccttta gtgagggtta ataaacttgg 
       61 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct 
      121 gaaaggagga actatatgcg ctcatacgat atgaacgttg agactgccgc tgagttatca 
      181 gtgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc 
      241 tgaaaggagg aactatatcc ggccggatag cttatcgcta gaggtcgaaa ttcacctcga 
      301 aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt ttttggagat 
      361 tttcaacgtg aaaaaattat tattcgcaat tccaagctaa ttcacctcga aagcaagctg 
      421 ataaaccgat acaattaaag gctccttttg gagccttttt ttttggagat tttcaacgtg 
      481 aaaaaattat tattcgcaat tccaagctct gcctcgcgcg tttcggtgat gacggtgaaa 
      541 acctctgaca catgcagctc cctaggcaat tgcatgtgag caaaaggcca gcaaaaggcc 
      601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
      661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
      721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
      781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
      841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
      901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
      961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     1021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     1081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     1141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     1201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     1261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     1321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     1381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     1441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     1501 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     1561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     1621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     1681 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 
     1741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     1801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     1861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     1921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     1981 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 
     2041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     2101 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     2161 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     2221 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttatgtaagc 
     2281 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt 
     2341 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat 
     2401 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa 
     2461 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga 
     2521 tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg 
     2581 ccggtgatgc cggccacgat gcgtccggcg tagaggatct ctcacctacc aaacaatgcc 
     2641 cccctgcaaa aaataaattc atataaaaaa catacagata accatctgcg gtgataaatt 
     2701 atctctggcg gtgttgacat aaataccact ggcggtgata ctgagcacat cagcaggacg 
     2761 cactgaccac catgaaggtg acgctcttaa aattaagccc tgaagaaggg cagcattcaa 
     2821 agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggaattccgg atctcgatcc 
     2881 cggaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt 
     2941 gtttaacttt aagaaggaga tatacatatg agcaatccgc ggtctttgga agaggagaaa 
     3001 tatgatatgt caggtgcccg cctggcccta atactgtgtg tcaccaaagc ccgggaaggt 
     3061 tccgaagaag acctggatgc tctggaacac atgtttcggc agctgagatt cgaaagcacc 
     3121 atgaaaagag accccactgc cgagcaattc caggaagagc tggaaaaatt ccagcaggcc 
     3181 atcgattccc gggaagatcc cgtcagttgt gccttcgtgg tactcatggc tcacgggagg 
     3241 gaaggcttcc tcaagggaga agatggggag atggtcaagc tggagaatct cttcgaggcc 
     3301 ctgaacaaca agaactgcca ggccctgcga gctaagccca aggtgtacat catacaggcc 
     3361 tgtcgaggag aacaaaggga ccccggtgaa acagtaggtg gagatgagat tgtgatggtc 
     3421 atcaaagaca gcccacaaac catcccaaca tacacagatg ccttgcacgt ttattccacg 
     3481 gtagagggat acatcgccta ccgacatgat cagaaaggct catgctttat ccagaccctg 
     3541 gtggatgtgt tcacgaagag gaaaggacat atcttggaac ttctgacaga ggtgacccgg 
     3601 cggatggcag aagcagagct ggttcaagaa ggaaaagcaa ggaaaacgaa ccctgaaatc 
     3661 caaagcaccc tccggaaacg gctgtatctg cagtag 
//
